This study focuses on refining in vitro propagation protocols for red currant cultivars of the Ribes genus and evaluating the role of LED lighting in the adaptation of microplants. The cultivars ‘Red Lake’, ‘Englische Grosse Weisse’, ‘Marmeladnitsa’, and ‘Podarok Leta’ were successfully introduced into in vitro culture during their dormancy phase using 0.1% HgCl2 as a sterilizing agent. The period of spring introduction is not very efficient in connection with the intensive development of saprophytic microflora and weak morphogenesis microplants. Using 0.01% C9H9HgNaO2S sterilizer resulted in a decrease in the necrosis percentage, but an increase in mold proportion. The preparation of the plants with 12% H2O2 was considered environmentally not effective enough to obtain a large number of healthy microplants. The use of 12% H2O2 resulted in increased necrosis rates by 24.76% compared to 0.01% C9H9HgNaO2S and 0.1% HgCl2 sterilizers. The variety specificity of Ribesia plants in terms of the content of MS and LF nutrient media components was determined by the survival rate of explants, the formation of additional micro-shoots, and the morphological development. The MS medium with the addition of 1 mg∙L−1 BAP decreased the percentage of mold and necrosis infection and provided a high percentage of viable plants with optimal growth and reproduction rate. In contrast, the LF medium with the same concentration of 6-BAP resulted in poor explant quality and leaf chlorosis at later stages. The study also investigated the effects of different LED light spectra on morphological and physiological traits. For ‘Red Lake’ and ‘Englische Grosse Weisse’, RWUV-A (625–740 nm) lighting enhanced biomass and chlorophyll (Chl a and Chl a + b) accumulation, while the White (W) spectrum benefited ‘Podarok Leta’. Conversely, the RW spectrum with minimal green and no ultraviolet light restricted growth and photosynthetic pigment accumulation across all cultivars, promoting compact plant structures. The RWUV-A lighting condition resulted in the highest NDVI values across all cultivars, indicating an improved physiological status and biomass accumulation. These findings underscore the importance of refining the microclonal reproduction protocols for Ribesia subgenus representatives, emphasizing the genotype-specific light modulation during the proliferation stage. The study highlights the utility of the MS medium and tailored light conditions in enhancing the effectiveness of propagation techniques for producing high-quality planting material.

This article proposes a new method for determining the pathological state of a plant, based on a combination of the method for measuring the dynamics of photosystem II pigment fluorescence in the leaves of L. sativa plants and analyzing the resulting time series using bispectral analysis based on the wavelet transform. The article theoretically shows a possible mechanism for the appearance of a peak on the map of bispectrum indexes during nonlinear analog conversion of a physiological signal in a biological object. The phenomenon of increasing the degree of nonlinearity in the transmission of an external periodic signal in plant signaling systems has been experimentally demonstrated.

Food insecurity is a top economic and national security concern in many countries, and scientists worldwide are working to increase crop productivity in order to address this issue. In line with this information, the present study aimed to test the possibility of improving the yield and fruit quality of two tomato cultivars, namely ‘Vspyshka’ and ‘Lyana’. The effect of LSL (light of sodium lamps—control) and the short additional 4 h treatment of nighttime LED lighting (LSL+night LED) with an increase in the proportion of red, blue, and far-red spectra on tomato fruit yield as well as its physiological, biochemical, and consumer attributes were compared in this study. The results suggested that LSL+night LED significantly increased soluble solids concentration, vitamin C content, and polyphenolic compounds of tomato fruits, taking into account the varietal characteristics. Moreover, a moderate to high relationship was also observed between the polyphenolic complex, vitamin C content, and antioxidant activity. It was concluded that the LSL+night LED could further enhance the relationship between polyphenols and antioxidants, as well as soluble solids concentration. LSL+night LED treatment also provided an increased accumulation of five essential amino acids associated with the taste characteristics of fruits, namely histidine, valine, threonine, licin, and the sum of isoleucine. In addition, the contents of lysine and methionine increased in the ‘Lyana’ cultivar. LSL+night LED treatment was also noted to have a less pronounced effect on the contents of aspartic acid and asparagine, as bio stimulators of plant growth processes, as well as the amino acids arginine, serine, glycine, and tyrosine, which were additionally consumed to restore photosynthesis. LSL+night LED treatment reduced the concentration of nitrates in fruits, which is a toxic element for human health. Overall, the results of the study are believed to be demanded in practical applications, with potential benefits in improving the elements of resource-saving technology for growing vegetable crops.

The oscillation synchronization analysis in biological systems will expand our knowledge about the response of living systems to changes in environmental conditions. This knowledge can be used in medicine (diagnosis, therapy, monitoring) and agriculture (increasing productivity, resistance to adverse effects). Currently, the search is underway for an informative, accurate and sensitive method for analyzing the synchronization of oscillatory processes in cell biology. It is especially pronounced in analyzing the concentration oscillations of intracellular signaling molecules in electrically nonexcitable cells. The bispectral analysis method could be applied to assess the characteristics of synchronized oscillations of intracellular mediators. We chose endothelial cells from mouse microvessels as model cells. Concentrations of well-studied calcium and nitric oxide (NO) were selected for study in control conditions and well-described stress: heating to 40 °C and hyperglycemia. The bispectral analysis allows us to accurately evaluate the proportion of synchronized cells, their synchronization degree, and the amplitude and frequency of synchronized calcium and NO oscillations. Heating to 40 °C increased cell synchronization for calcium but decreased for NO oscillations. Hyperglycemia abolished this effect. Heating to 40 °C changed the frequencies and increased the amplitudes of synchronized oscillations of calcium concentration and the NO synthesis rate. The first part of this paper describes the principles of the bispectral analysis method and equations and modifications of the method we propose. In the second part of this paper, specific examples of the application of bispectral analysis to assess the synchronization of living cells in vitro are presented. The discussion compares the capabilities of bispectral analysis with other analytical methods in this field.

The research was carried out in 2019 - 2020 in the depart tent of closed artificial agroecosystems for plant growing in the Federal State Budgetary Scientific Institution "Federal Scientific Agroengineering Center VIM", Moscow. The study focused on the seeds of tomatoes (Solanum lycopersicum L.) variety "Lyana". Amber acid was used as a biostimulating factor at the stage of pre-sowing seed treatment (factor A) and with constant drip irrigation of plants (factor B) throughout the entire growing season - 145 days. The use of amber acid can increase the crop yield from 2.34 to 3.84 kg/bush when growing tomatoes under controlled conditions in a climatic chamber. Inoculation of seeds with a biostimulant substance promoted an increase in the content of vitamins C (23.23 mg/100 g) and B-1 (0.041 mg/100 g) and dry matter (5.06 %) compared to the control. As a result of determining the content of nitrates, it was revealed that the maximum permissible concentration in all samples did not exceed the permissible norm. Thus, the use of amber acid is very promising for growing Lyana tomato plants in artificial agroecosystems.

As a result of studies, it was found that collection samples of berry crops in vitro can be maintained in viable condition on specially selected nutrient media for 12 months. The effect of mannitol concentration (0.45, 0.75 and 1.05%; sucrose control -3%) on the regenerative potential of microprobe of the studied berry crop varieties was estimated. When studying the effect of the reproduction cycle on the morphogenesis of meristemic cultures, it was found that the dynamics of changes in shoot length, number of leaves and shoots depended on the concentration of the active substance. In general, the majority of berry crops that were viable after storage of explants retained the ability to grow again, in many cases reaching or exceeding the control level.

ISHS VIII International Scientific and Practical Conference on Biotechnology as an Instrument for Plant Biodiversity Conservation (Physiological, Biochemical, Embryological, Genetic and Legal Aspects) Biotechnological methods of maintaining collections of the genus Ribes L.

The analysis of contemporary research on molecular marking and genetic certification for use in breeding, biotechnology and identification of horticultural crops is carried out. In Russia and abroad, active work is underway on the identification and certification of garden crops: apple, pear, various types of stone fruit crops, raspberry, strawberry, currant and gooseberry. Currently, the most effective and frequently used are SSR markers. Genetic certificates have been elaborated for many fruit and small fruit crops, which are used in breeding research, works on the study of genetic diversity, in variety diagnosis and diagnosis of pathogens and genealogy analysis. In previous studies using SSR markers, 16 apple varieties, 10 cherry varieties, 29 raspberry varieties and 12 pear varieties of ARHIBAN contemporary breeding were genotyped. The appearance of plant genetic certificates contributed to the development of marker-oriented breeding, making it possible to identify and select genotypes carrying target genes and quantitative trait loci (QTLs) using only DNA analysis data without preliminary phenotypic evaluation. Molecular genetics certificate can serve as a reliable tool to protect the copyright of breeders. In conditions of Russian Federation it is necessary to expand researches of genomic analysis of fruit and small fruit crops, improve and unify the methods of DNA identification and molecular marking techniques, develop common requirements for the level of information content of markers, principles and methods of evaluation of planting material and collections in vitro. The researchers are faced with the task of creating a clear system of molecular-genetic identification and certification of planting material, which will allow to develop and introduce into production varieties with known characteristics, to control plant material at all stages of nursery and commercial distribution of varieties.

the paper presents the results on the development and preservation of black currant promising cultivars from the genetic collection of the given institution, using cultivar samples that are bred by the leading Russian scientific institutions. there was started a research on creating and maintaining Ríbes l. collection in vitro; at the moment, depositing technology is being practiced. Currently, our collection fund has 31 black currant cultivars, 10 of which are introduced into in vitro. the creation of in vitro bank not only helps to develop the research on preserving plant biodiversity, but also is the basis for studying fundamental problems in plants physiology and biochemistry, including genetic and breeding works.

Using polyacrylamide gel electrophoresis in the glycine-acetic acid system (pH 3.2), variants of proteins of white-lupine seeds were revealed. The study of conglutin polymorphism in the culture of the autogamous population F→∞ (var. Dega) revealed two loci, Con A and Con B, which control protein synthesis. The loci were situated in the same linkage group within a distance of 11.48 ± 3.4% of recombination. Natural selection in favor of genotypes that contain Con A1 and Con B2 alleles is proposed. It is established that conglutins A and B (CON A and CON B) contain cysteine residues, which form intermolecular disulfide bonds between peptides.