Introduction. The optimal method of obtaining and application a stromal-vascular fraction (SVF) of adipose tissue in the treatment of donor wounds in burned patients has not been determined.Objective — to compare the effectiveness of the stromal-vascular fraction of adipose tissue in the treatment of donor wounds, depending on the method of obtaining (mechanical and enzymatic) and the injection (subdermal, intradermal) into the tissue.Material and methods. Wistar rats (n = 30) were divided into 2 investigated groups: in the first group (eSVF) enzymatic processing of adipose tissue (n = 10) was used, in the second group (mSVF) mechanical processing was used (n = 10). Another 10 rats were used as adipose tissue donors’ group. A deep burn was created in all animals, and after 4 days two more donor wounds were created by taking a split thickness skin autograft: the stromal-vascular fraction was injected intradermally into one of the wounds, subdermally into the other. After 14 days, the area of epithelialization, the microcirculation index and the histological microstructure of the skin were evaluated.Results. The proportion of completely epithelized donor wounds in the eSVF-group was 85 %, in the mSVF-group — 55 % (p < 0.05). The index of microcirculation significantly decreased after intradermal injections in the eSVF-group (p < 0.01), and after subdermal injections — in the mSVF-group (p < 0.05). According to histomorphometry, with intradermal injections of eSVF, the thickness of the epidermis and the layer of granulation tissue is less than in the mSVF group (p < 0.01). At the same time, the relative density of collagen fibers in the granulation tissue was significantly higher after the injections of eSVF than after mSVF (p < 0.01). Comparison of injections of the stromal-vascular fraction showed: no significant differences were found when using eSVF; when using mSVF, the subdermal injections are preferable, since it was accompanied by a significantly lower thickness of granulation tissue (p < 0.01).Conclusions. The enzymatically obtained stromal-vascular fraction of adipose tissue has advantages over the mechanically obtained one: the efficiency of eSVF is equally high when used with intradermally and subdermally injections, which are confirmed by the structure and blood circulation in the newly formed skin. When using mSVF, intradermal injections are the least effective, and subdermal injections are accompanied by a moderately pronounced positive dynamic in the microstructure of the skin of donor wounds.

e-mail: daleynik@yandex.ru Burn injury with a lesion of a large area of the body surface remains a serious problem for surgeons. Every year In Russia about 400 thousands of people receive burns of different severity level, among which about 30 % of people need hospitalization. In recent decades, methods of regenerative medicine have been actively used to treat burn injuries. One of the promising areas to treat burns are technologies based on the use of uncultivated freshly harvested autologous skin cells. The experience of treating of 14 patients with extensive burns of 20–80 % is presented. To restore the lost of skin cover a suspension of freshly isolated autologous skin cells in combination with a 1: 6 mesh graft and fibrin glue was used. The suspension of cells was obtained by gentle cold trypsinization. It is shown that keratinocytes and fibroblasts are included in the obtained cell suspension, cells’ viability is more than 60 % and cells’ yield from 1 cm 2 is about 6 million. The completion of epithelialization in the cells of the autografts within 6-14 days after cells’ transplantation is recorded. Following observation for the patients within 12 months after discharge form the hospital showed satisfactory cosmetic and