The antiviral effect of 2',5'-trioligoadenylate (2',5'-A(3)) and some of its analogues was studied using several model cell culture systems and viruses: mice L929 fibroblast cells inoculated with vaccine virus, testicular piglet cells inoculated with Aueszki disease virus (strain BUK-628), and the same culture inoculated with a reference strain of transmissible.gastroenteritis virus, strain Purdue - 115. Our results suggest that both 2',5'-trioligoadenylate and its analogues are promising antiviral substances against DNA-and RNA-containing viruses.

The effect of trimeric 2',5'-oligoadenylic acid (2',5'A(3)) and its epoxy-derivative (2',5'-A(2)((RA)A) on human immunodeficiency virus (HIV-1) reproduction was studied. An HIV-1 infectivity titer decrease was shown on the model of limphoblastoid cells when the substances under study were used. The interferonogenic effect of both substances was discovered. 2',5'-A(2)((RA)A) inhibited the activity of retrovirus reverse transcriptase (a C-type).

Two non-conventional analogues of ATP, 3'-deoxyadenosine-2'-triphosphate (3'-d-2'-ATP) and 2'-deoxyadenosine-3'-triphosphate (2'-d-3'-ATP), the syntheses of which are described, were examined as potential phosphate donors for the nucleoside kinases: 2'-deoxycytidine kinase (dCK), cytosolic thymidine kinase (TK1) and mitochondrial thymidine kinase (TK2). The reactions were monitored by means of a mixture of [gamma-32P]ATP and cold analogue, and/or with the use of 3H-labelled acceptors and cold donor. With dCK, using equimolar mixtures of ATP with each analogue, and dC as acceptor, phosphate transfer from 3'-d-2'-ATP and 2'-d-3'-ATP amounted to 34% and 14%, respectively. With each analogue used alone (each at concentration of 100 microM), phosphate transfer from 3'-d-2'-ATP was 55% that from ATP, and from 2'-d-3'-ATP 16%. With human TK2, and equimolar mixtures of [gamma-32P]ATP with each of the analogues, and 1 microM dT as acceptor, there was no detectable transfer from either analogue. But, when each analogue was used alone, phosphate transfer attained 11% and 5%, respectively, that for ATP alone. With the low affinity form of human TK1, and dT as acceptor, only low phosphate transfer occurred with either analogue used alone. Both compounds exhibited Michaelis-Menten kinetics (with significantly lower Vmax than ATP), while ATP exhibited cooperative kinetics with all three kinases.

ChemInformVolume 20, Issue 14 Natural Products ChemInform Abstract: One-Step Synthesis of Pyrimidine 2′-Deoxy-5′-O-trityl-O2,3′-anhydronucleosides. G. V. ZAITSEVA, G. V. ZAITSEVA Inst. bioorg. khim. Akad. nauk Beloruss. SSR MinskSearch for more papers by this authorE. V. VAAKS, E. V. VAAKS Inst. bioorg. khim. Akad. nauk Beloruss. SSR MinskSearch for more papers by this authorV. E. PASHINNIK, V. E. PASHINNIK Inst. bioorg. khim. Akad. nauk Beloruss. SSR MinskSearch for more papers by this authorV. I. TOVSTENKO, V. I. TOVSTENKO Inst. bioorg. khim. Akad. nauk Beloruss. SSR MinskSearch for more papers by this authorL. N. MARKOVSKII, L. N. MARKOVSKII Inst. bioorg. khim. Akad. nauk Beloruss. SSR MinskSearch for more papers by this authorI. A. MIKHAILOPULO, I. A. MIKHAILOPULO Inst. bioorg. khim. Akad. nauk Beloruss. SSR MinskSearch for more papers by this author G. V. ZAITSEVA, G. V. ZAITSEVA Inst. bioorg. khim. Akad. nauk Beloruss. SSR MinskSearch for more papers by this authorE. V. VAAKS, E. V. VAAKS Inst. bioorg. khim. Akad. nauk Beloruss. SSR MinskSearch for more papers by this authorV. E. PASHINNIK, V. E. PASHINNIK Inst. bioorg. khim. Akad. nauk Beloruss. SSR MinskSearch for more papers by this authorV. I. TOVSTENKO, V. I. TOVSTENKO Inst. bioorg. khim. Akad. nauk Beloruss. SSR MinskSearch for more papers by this authorL. N. MARKOVSKII, L. N. MARKOVSKII Inst. bioorg. khim. Akad. nauk Beloruss. SSR MinskSearch for more papers by this authorI. A. MIKHAILOPULO, I. A. MIKHAILOPULO Inst. bioorg. khim. Akad. nauk Beloruss. SSR MinskSearch for more papers by this author First published: April 4, 1989 https://doi.org/10.1002/chin.198914319AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article. Volume20, Issue14April 4, 1989 RelatedInformation

Chemischer InformationsdienstVolume 14, Issue 28 Heterocyclic Compounds ChemInform Abstract: DIRECT FLUORINATION OF 3-METHOXYCARBONYLPYRAZOLE E. I. KVASYUK, E. I. KVASYUKSearch for more papers by this authorI. A. MIKHAILOPULO, I. A. MIKHAILOPULOSearch for more papers by this authorN. E. PUPEIKO, N. E. PUPEIKOSearch for more papers by this authorD. TSEKH, D. TSEKHSearch for more papers by this author E. I. KVASYUK, E. I. KVASYUKSearch for more papers by this authorI. A. MIKHAILOPULO, I. A. MIKHAILOPULOSearch for more papers by this authorN. E. PUPEIKO, N. E. PUPEIKOSearch for more papers by this authorD. TSEKH, D. TSEKHSearch for more papers by this author First published: July 12, 1983 https://doi.org/10.1002/chin.198328193Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume14, Issue28July 12, 1983 RelatedInformation