The gene encoding the soluble pyridine nucleotide transhydrogenase (STH) of Azotobacter vinelandii was cloned and sequenced. This is the third sth gene identified and further defines a new subfamily within the flavoprotein disulfide oxidoreductases. The three STHs identified all lack one of the redox active cysteines that are characteristic for this large family of enzymes, and instead they contain a conserved threonine residue at this position. The recombinant A. vinelandii enzyme was purified to homogeneity and shown to form filamentous structures different from those of Pseudomonas fluorescens and Escherichia coli STH. Chimeric STHs were constructed which showed that the C-terminal region is important for polymer formation. The A. vinelandii STH containing the C-terminal region of P. fluorescens or E. coli STH showed structures resembling those of the STH contributing the C-terminal portion of the protein.

ABSTRACT The udhA gene of Escherichia coli was cloned and expressed in E. coli and found to encode an enzyme with soluble pyridine nucleotide transhydrogenase activity. The N-terminal end of the enzyme contains the fingerprint motif of a dinucleotide binding domain, not present in published E. coli genome sequences due to a sequencing error. E. coli is hereby the first organism reported to possess both a soluble and a membrane-bound pyridine nucleotide transhydrogenase.

Previous articleNext article No AccessRock Art Recording and Conservation: A Call for International EffortEmmanuel Anati, Ian Wainwright, and Doris LundyEmmanuel Anati Search for more articles by this author , Ian Wainwright Search for more articles by this author , and Doris Lundy Search for more articles by this author PDFPDF PLUS Add to favoritesDownload CitationTrack CitationsPermissionsReprints Share onFacebookTwitterLinkedInRedditEmail SectionsMoreDetailsFiguresReferencesCited by Current Anthropology Volume 25, Number 2Apr., 1984 Sponsored by the Wenner-Gren Foundation for Anthropological Research Article DOIhttps://doi.org/10.1086/203111 Views: 4Total views on this site Citations: 3Citations are reported from Crossref Copyright 1984 The Wenner-Gren Foundation for Anthropological ResearchPDF download Crossref reports the following articles citing this article:Aaron M. Wright , Journal of Archaeological Method and Theory 25, no.33 ( 2018): 911.https://doi.org/10.1007/s10816-017-9363-xMélanie Duval, Benjamin W. Smith Inscription au patrimoine mondial et dynamiques touristiques : le massif de l'uKhahlamba-Drakensberg (Afrique du Sud), Annales de géographie n° 697, no.33 (Jun 2014): 912–934.https://doi.org/10.3917/ag.697.0912Mélanie Duval, Benjamin Smith Rock art tourism in the uKhahlamba/Drakensberg World Heritage Site: obstacles to the development of sustainable tourism, Journal of Sustainable Tourism 21, no.11 (Jan 2013): 134–153.https://doi.org/10.1080/09669582.2012.699060