Open-circuit whole-room indirect calorimetry is a reliable method for assessing cumulative energy expenditure and substrate oxidation over a prolonged period without disrupting normal activities such as eating and sleeping. Over the past three decades, efforts to improve the time resolution of indirect calorimetry have increased, aiming to synchronize energy metabolism with rapidly changing behaviors and physiologic signals. As such, various new algorithms have been proposed: trend identification, total variation denoising, wavelet de-noising, Kalman estimation, and deconvolution with a regularization parameter. The ability to estimate the time course of energy metabolism differs among algorithms. In this review on the methodology of open-circuit whole-room indirect calorimetry, we discuss similarities and critical differences in the theoretical background of various algorithms and compare their performance in simulations. In terms of correlation with the true value and mean squared errors, deconvolution with a regularization parameter outperformed the other algorithms. As further improvements in the algorithmic approaches are expected, we propose a practical guideline to support ongoing development: newly developed algorithms should be benchmarked against existing algorithms, and the source code should be made publicly available to facilitate transparent evaluation and future comparisons.

Sleep is influenced by environmental factors, and hot ambient temperature undermines sleep quality. Considering the global warming, it becomes increasingly important to keep bedroom temperature cool in the summer. Today, two electrical cooling systems are available: convection and radiant. Convection air cooling system supplies cooled air-flow. Radiant cooling system cools the room through radiation from the cooled surfaces, moisture condensation on which is prevented by reheated refrigeration cycle system. The present study was a randomized, cross-over trial setting room air temperature at 26 ℃ to compare subjective and objective quality of sleep between convection and radiant cooling in 7 healthy women. Subjective and objective quality of sleep were assessed by Oguri-Shirakawa-Azumi Sleep Inventory and EEG-based sleep parameters, respectively. Compared with convection cooling, radiant cooling provided lower relative humidity and air flow. Refreshness, one of the 4 indices of subjective quality of sleep, was higher with radiant cooling. Among the EEG-based sleep parameters, sleep efficiency was higher and sleep latency was shorter with radiant cooling. In spite of similar ambient room temperature, difference in cooling (radiant vs. convection) and/or dehumidifying (ordinary air conditioning vs. reheated refrigeration cycle) method provided difference in thermal comfort affecting subjective and objective quality of sleep.

Delta power in electroencephalography during sleep has been used as a more quantitative approach to determine sleep depth and quality. Despite the positive effects of both exercise and cognitive activity on delta power during sleep, limited information is available regarding the effects of multi-task exercise, which combines these two activities. Therefore, this study aimed to investigate the impact of multi-task exercise on delta power during sleep. Fifteen older female adults participated in both multi-task and single-task exercises at low and moderate-to-vigorous intensities for 30 min each. Sleep architecture was recorded using polysomnography to compare the amount of delta power during sleep. Additionally, cortical hemodynamic changes in the prefrontal cortex were monitored using functional near-infrared spectroscopy before and after each exercise session. Low-intensity multi-task exercise increased cortical activation in the right and left ventrolateral prefrontal cortex and frontopolar area, which positively correlated with the increase in delta power. This study provides the first experimental evidence that low-intensity multi-task exercise enhances cortical activation in the prefrontal cortex of older female adults, leading to an increase in delta power during sleep. It also suggests that low-intensity multi-task exercise may be a more useful intervention for improving sleep quality in older adults.

This comparative study focused on chronic exercise training, menstrual cycle, menstruation, and sex related with slow-wave sleep and energy expenditure (EE) during sleep in runners and controls. Participants included 26 highly trained runners (9 males, 8 amenorrheic females, and 9 menstruating females) and 20 controls (10 males and 10 menstruating females) aged 21 +/- 2 years. Sleeping metabolic rate and EE during slow- wave sleep were 2.7% and 3.9% higher in the luteal than the follicular phase for female runners. Significant interactions were found between the 8-h time course adjusted EE and menstruation, exercise training, and sex. Sleep stage durations did not differ between groups. Interestingly, amenorrheic runners did not suppress sleeping and overnight metabolic rates, suggesting that EE in sleep may not be a causative factor for amenorrhea in runners. This study highlights the complex relationship between chronic exercise, menstrual cycle, and sex on EE during sleep.

Objectives: Insomnia is a common sleep disorder, and various strategies have been explored for its treatment. One of the methods involves the administration of carbon dioxide (CO2) at bedtime, as the CO2 increment exerts a sedative effect on the central nervous system. We aimed to assess the effect of CO2 on sleep quality in patients with insomnia using the coefficient of variation of the envelope (CVE) analysis, a novel analytical method. Methods: In this randomized, double-blind, sham-controlled crossover trial, 24 adults with sleeping difficulties underwent level 1 polysomnography. Two interventions involved exposure to either 2% low-concentration CO2 gas or room air. CVE analysis was performed to assess the stability of the delta band. Morphological variations in delta waves can be quantitatively monitored using the envelope analysis, which assesses the effect of manipulations on sleep that may otherwise elude detection. Results: Exposure to CO2 improved the total sleep time (TST) (p=0.010) and total arousal index (TAI) (p=0.011). The CVE analysis showed a distinct distribution between the experimental and sham-controlled groups. Moreover, correlations between the TST and CVE (r=0.41) and those between the TAI and CVE (r=0.40) were observed. Conclusions: This study is the first to evaluate the effects of CO2 exposure on sleep stability in patients with insomnia. The CVE analysis implies the possibility of sleep stabilization by CO2 administration and suggests a link between enhanced sleep maintenance and sleep stability.

The present study investigated the potential role of the composition of dietary fatty acids in the regulation of biological rhythms, such as the sleep architecture, core body temperature and leukocyte clock gene expression, in subjects fed meals rich in palmitic acid (PA) or oleic acid (OA). Eleven males participated in two sessions of indirect calorimetry in a whole-room metabolic chamber. In each session, subjects consumed three meals rich in PA (44·3 % of total fat as PA and 42·3 % as OA) or OA (11·7 % of total fat as PA and 59·3 % as OA) in the metabolic chamber. The ratio of PA to OA in plasma was significantly lower and fat oxidation was significantly higher during 24 h of indirect calorimetry in the session with meals rich in OA than in that with meals rich in PA. The duration of slow wave sleep (SWS) was shorter, the latency of SWS was longer and the nadir of core body temperature after bedtime was later in the session with meals rich in PA than in that with meals rich in OA. The peak in CRY1 gene expression was earlier and its amplitude was higher in the session with meals rich in PA than in that with meals rich in OA. In healthy young males, meals rich in PA decreased fat oxidation and disrupted biological rhythms, particularly the sleep architecture and core body temperature during sleep, more than meals rich in OA.

Suvorexant is an orexin receptor antagonist that targets the wake-promoting system. Orexin is also known to regulate energy metabolism in rodents, but its role in humans remains largely unknown. Here, we assessed the effect of suvorexant (20 mg) on energy metabolism during sleep and shortly after awakening in a randomized, double-blind, placebo-controlled, crossover study in 14 healthy men. Suvorexant increased rapid eye movement (REM) but decreased nonrapid eye movement (NREM) stage 1. Energy expenditure during wake after sleep onset (WASO) was higher than that during NREM and REM sleep in the placebo but not in the suvorexant trial, suggesting that the increase in energy expenditure during WASO was due to an activation of the orexin system. Fat oxidation during sleep increased, and its effect remained after waking the next morning. Suvorexant decreased protein catabolism but did not affect overall energy expenditure. The orexin system may affect fat oxidation independent of its roles in sleep regulation in humans.

There is a physiological link between sleep and eating. Insufficient sleep is a risk factor for overeating and excess body weight gain, and molecules such as orexin and insulin play a role in the control of sleep and energy intake. The effects of dietary timing on sleep and energy metabolism were examined in this review. First, we examined sleep energy metabolism and sleep quality under time-restricted eating, including skipping breakfast or dinner. Second, the mechanisms, benefits, and translational potential of the effects of time-restricted diets on sleep were discussed. Time-restricted eating under controlled conditions, in which daily caloric intake was kept constant, affected the time course of energy metabolism but did not affect total energy expenditure over 24 h. In free-living conditions, time-restricted eating for extended durations (4–16 weeks) decreased energy intake and body weight, and the effects of early time-restricted eating were greater than that of midday time-restricted eating. Although assessment of sleep by polysomnographic recording remains to be performed, no negative effects on the subjective quality of sleep have been observed.

대사증후군은 복부 비만, 고혈압, 고 중성지방혈증, 저 고밀도지단백질 콜레스테롤혈증 및 인슐린 저항성의 5가지 위험 요인 중 3가지 이상의 군집으로 정의되며, 전 세계 인구의 20% 이상에게 영향을 미치는 것으로 평가된다. 비만은 대사증후군의 주요 위험 요인이며, 산화 스트레스와 그에 따른 염증은 대사증후군의 모든 구성 요소에서 주요 병리생리학적 역할을 한다. 운동은 대사증후군 환자의 신체 구성, 심혈관 및 대사 결과를 향상시킨다. 운동은 대사증후군 환자에게 이롭지만, 현재로서 최적의 운동량 및 운동 형태는 정립되지 않았다. 대사증후군에 있어 유산소 운동과 저항 운동의 복합 운동에 대한 많은 연구가 필요하다.

Core body temperature (CBT) shows a diurnal rhythm, and the nocturnal decrease in CBT is blunted in older people. The physiological mechanisms responsible for the blunted nocturnal decrease in CBT in older people remain to be revealed. The aim of this study was to compare heat production and heat dissipation in young and old subjects during sleep, as assessed by indirect calorimetry and the distal–proximal temperature gradient (DPG) of skin temperature. A complete dataset of 9 young (23.3 ± 1.1 years) and 8 old (72.1 ± 2.5 years) females was analyzed. CBT and energy metabolism were monitored during sleep using an ingestible temperature sensor in a metabolic chamber maintained at 25 °C. Skin temperature was measured at proximal and distal parts of the body. CBT, distal skin temperature, and DPG in older subjects were higher than in young subjects. Protein oxidation was similar between the two groups, but fat oxidation was lower and carbohydrate oxidation was higher in old subjects compared to young subjects. On the other hand, energy expenditure was similar between the two age groups. Thus, the elevated CBT in older subjects was not attributed to deteriorated heat dissipation or enhanced heat production, suggesting an alternative explanation such as deteriorated evaporative heat loss in old subjects.

Study objective Traditionally, age-related deterioration of sleep architecture in older individuals has been evaluated by visual scoring of polysomnographic (PSG) recordings with regard to total sleep time and latencies. In the present study, we additionally compared the non-REM sleep (NREM) stage and delta, theta, alpha, and sigma wave stability between young and older subjects to extract features that may explain age-related changes in sleep. Methods Polysomnographic recordings were performed in 11 healthy older (72.6 ± 2.4 years) and 9 healthy young (23.3 ± 1.1 years) females. In addition to total sleep time, the sleep stage, delta power amplitude, and delta, theta, alpha, and sigma wave stability were evaluated by sleep stage transition analysis and a novel computational method based on a coefficient of variation of the envelope (CVE) analysis, respectively. Results In older subjects, total sleep time and slow-wave sleep (SWS) time were shorter whereas wake after sleep onset was longer. The number of SWS episodes was similar between age groups, however, sleep stage transition analysis revealed that SWS was less stable in older individuals. NREM sleep stages in descending order of delta power were: SWS, N2, and N1, and delta power during NREM sleep in older subjects was lower than in young subjects. The CVE of the delta-band is an index of delta wave stability and showed significant differences between age groups. When separately analyzed for each NREM stage, different CVE clusters in NREM were clearly observed between young and older subjects. A lower delta CVE and amplitude were also observed in older subjects compared with young subjects in N2 and SWS. Additionally, lower CVE values in the theta, alpha and sigma bands were also characteristic of older participants. Conclusion The present study shows a decrease of SWS stability in older subjects together with a decrease in delta wave amplitude. Interestingly, the decrease in SWS stability coincided with an increase in short-term delta, theta, sigma, and alpha power stability revealed by lower CVE. Loss of electroencephalograms (EEG) variability might be a useful marker of brain age.

Although recent studies have examined the bidirectional associations between physical activity and sleep parameters, few have focused on older adults utilizing objective assessments, such as polysomnography. This micro-longitudinal observational study included 92 Japanese older adults (aged 65–86 years) who underwent objective evaluations of sleep quality using polysomnography and completed subjective sleep-related questionnaires. Activity levels were assessed using an accelerometer. Polysomnography, subjective sleep-related questionnaires, and accelerometer were administered for 7 consecutive days. Multilevel models (participant-, day-level) were used to examine the temporal associations of objective and subjective sleep parameters with sedentary behavior and physical activity. In the day-level analysis, higher levels of sedentary behavior during daytime were associated with longer rapid eye movement (REM) sleep, shorter REM latency, lower levels of non-REM sleep (stage N3), and reduced delta power during daytime. Higher levels of low-intensity physical activity during daytime were associated with lower levels of REM sleep, longer REM latency, and increased stage N3 sleep in the day-level analysis. Higher levels of moderate-to-vigorous physical activity were associated with increased REM latency. Longer subjective sleep time was associated with increased next-day moderate-to-vigorous physical activity. Thus, low-intensity physical activity may provide objective benefits related to deep sleep parameters in older adults.

Introduction Current hypnotic agents have next-day residual effects. The new orexin antagonist, suvorexant, has little muscle relaxation effect on the physical and cognitive function in the following morning and daytime. In this study, the effects of suvorexant, zolpidem, ramelteon and placebo in elderly subjects were evaluated. Methods Six men and eight women aged 63-75 years received a single tablet and lights were then turned off. Subjects were instructed to sleep from 23:00-6:00 with an interruption from 4:00-4:30 for evaluations. Suvorexant 10 mg, zolpidem 5 mg, ramelteon 4 mg or placebo was administered single time in a randomized, double-blind and crossover design with a one-week drug holiday in between each drug. Measures of objective parameters and subjective ratings were obtained every 2 h from 4:00 to 16:00. Result No subjects showed serious side effects from physical observations and vital sign checks before and after hypnotics were taken. During the first sleep period, the REM sleep time with suvorexant was especially longer than that with zolpidem. During the second sleep period, suvorexant had shorter sleep latency and longer stage2 sleep time than ramelteon and zolpidem, respectively. During the whole entire sleep, the REM sleep time with suvorexant was longer than zolpidem and placebo. For the body sway test with closed eye, the main effects of the medicines and zolpidem were significantly better than suvorexant and ramelteon. Conclusion The changes of physical and cognitive functions in healthy elderly after taking hypnotics were not remarkable. Therefore, these three hypnotics maybe appropriate for the elderly people with insomnia for single-time low dose administration.

Mammals and birds seek a warm environment prior to sleep, which triggers vasodilatation and body cooling. Ambient temperatures outside the thermoneutral zone suppress sleep, particularly rapid eye movement (REM) sleep. We discuss the neurocircuit interactions associated with thermal and sleep regulation that occur primarily in the hypothalamic areas. An increase in ambient temperature stimulates the median preoptic/medial preoptic area of the hypothalamus, decreases body temperature, and increases non-REM sleep. Similarly, optical stimulation of the ventrolateral preoptic nucleus, which contains galanin (VLPOGAL), results in body cooling and non-REM sleep. A decrease in VLPOGAL disrupts sleep in elderly individuals and may also be associated with reduced decline in core body temperature during sleep. However, stimulation of neurons that synthesize melanin-concentrating hormone in the lateral hypothalamus decreases body temperature and induces REM sleep. We also discussed the acute effect of light on sleep induction and decreased body temperature, implicated with gamma-aminobutyric acid-ergic neurons in the preoptic area. Further investigation is needed to determine the mechanisms that induce physiological responses in diurnal and nocturnal species. These studies will contribute to a better understanding of the association between sleep and thermoregulation.

Exposure to continuous light at night, including night-shift work or a nocturnal lifestyle, is emerging as a novel deleterious factor for weight gain and obesity. Here, we examined whether a single bout of bright light (BL) exposure at night affects energy metabolism via changes in circadian rhythm and nocturnal melatonin production. Ten healthy young men were randomized to a two-way crossover experimental design protocol: control (< 50 lux) and BL (approximately 10000 lux) conditions, with at least seven days of interval. The participants were exposed to each condition for 3 h (21:00-24:00) before sleep (0 lux, 00:00-07:00) in a room-type metabolic chamber. On each experimental night (21:00-07:00), energy expenditure, respiratory quotient (RQ), and substrate oxidation were measured to determine the energy metabolism. BL exposure prior to bedtime altered biological rhythms, disrupted the nocturnal decline in body temperature, and suppressed the melatonin level before sleeping, resulting in an increase in sleep latency. Indirect calorimetry data revealed that BL exposure significantly decreased the fat oxidation and increased the RQ, an indicator of the carbohydrate-to-fat oxidation ratio, throughout the whole period (light exposure and sleep). We revealed that acute BL exposure prior to bedtime exacerbated circadian rhythms and substrate oxidations, suggesting that chronic BL exposure at night may lead to obesity risk due to disturbances in circadian rhythms and macronutrient metabolism.

Known as metabolic flexibility, oxidized substrate is selected in response to changes in the nutritional state. Sleep imposes an extended duration of fasting, and oxidized substrates during sleep were assumed to progressively shift from carbohydrate to fat, thereby gradually decreasing the respiratory quotient (RQ). Contrary to this assumption, whole-room indirect calorimetry with improved time resolution revealed that RQ re-ascended prior to awakening, and nadir of RQ in non-obese young adults occurred earlier in women than men after bedtime. The transient decrease in RQ during sleep was blunted in metabolically inflexible men with smaller amplitude of diurnal rhythm in RQ. Similarly, the effect of 10 years difference in age on RQ became significant during sleep; the decrease in RQ during sleep was blunted in older subjects. Inter-individual difference in RQ become apparent during sleep, and it might serve as a window to gain insight into the early-stage pathogenesis of metabolic inflexibility.

노쇠는 생리적 기전의 저하에 의해 발생하는 노인 증후군으로 노화에 따른 신체 활동의 감소는 운동 능력을 감소시켜 노쇠를 유발하지만, 규칙적인 운동으로 노쇠를 예방 및 개선할 수 있다. 이에 본 연구에서는 노쇠를 예방하고 개선할 수 있는 최적의 운동 지침에 관해 고찰하였다. 유산소, 저항, 균형 및 유연성 운동이 포함된 복합 운동이 전-노쇠 및 노쇠 노인에게 권장된다. 전-노쇠 노인은 45-60분의 운동을 주 2-3회 실시해야 하며, 유산소, 저항, 균형 및 유연성 운동이 포함되어야 하지만 저항 및 균형 운동이 강조되어야 한다. 노쇠 노인은 유산소 운동이 강조된 30-45분의 복합 운동을 주 2-3회 실시해야 한다. 유산소, 균형 및 유연성 운동은 전-노쇠 및 노쇠 노인 모두 보그 척도를 이용한 운동 자각도에서 12-14점에 해당하는 강도로 시행해야 한다. 저항 운동은 1 repetition maximum(RM)의 55%로 시작하여 기능적 이점을 최대화하기 위해 1RM의 80% 강도로 증가시켜야 한다.

Abstract Exercise can improve sleep by reducing sleep latency and increasing slow-wave sleep (SWS). Some studies, however, report adverse effects of exercise on sleep architecture, possibly due to a wide variety of experimental conditions used. We examined the effect of exercise on quality of sleep using standardized exercise parameters and novel analytical methods. In a cross-over intervention study we examined the effect of 60 min of vigorous exercise at 60% $$\dot{V}{\text{O}}_{2}$$ V ˙ O 2 max on the metabolic state, assessed by core body temperature and indirect calorimetry, and on sleep quality during subsequent sleep, assessed by self-reported quality of sleep and polysomnography. In a novel approach, envelope analysis was performed to assess SWS stability. Exercise increased energy expenditure throughout the following sleep phase. The subjective assessment of sleep quality was not improved by exercise. Polysomnography revealed a shorter rapid eye movement latency and reduced time spent in SWS. Detailed analysis of the sleep electro-encephalogram showed significantly increased delta power in SWS (N3) together with increased SWS stability in early sleep phases, based on delta wave envelope analysis. Although vigorous exercise does not lead to a subjective improvement in sleep quality, sleep function is improved on the basis of its effect on objective EEG parameters.