It is possible to describe the pressure degradation of microorganisms as being analogous to thermal inactivation. Equation for baroinactivation is derived from thermal death time (TDT) model D-P = D-pref 10((pref-p)/zp), where D-pref is decimal reduction time at the reference pressure (min), p(ref) is the reference pressure (MPa), z(p) is the pressure increase (MPa) required to reduce the D-p value by a factor of 10. This method was used for the calculation of baroinactivation model parameters of the total number of microorganisms of pressurized germinated mung bean (green gram) seeds (Vigna radiata (L.) Wilczek). Microbial contamination of germinated mung bean (green gram) seeds can be effectively decreased by treatment at a high pressure of 400 MPa, time of pressurization 5 min.

High-pressure processing on germinated legume seeds (pea, chickpea, lentil, green gram) was studied. Total microflora was decreased: in three days germinated pea seeds decreased from 2.4 x 10(6) CFU/g to less than 10; in two days germinated chickpea seeds decreased from 1.6 x 10(5) CFU/g to less than 10; in three days geminated lentil seeds decreased from 3.4 x 10(7) CFU/g to less than 10; and in three days germinated green gram decreased from 1.4 x 10(9) to 1.8 X 10(2) after treatment of high pressure 500 MPa for 10 min. High pressure treated germinated seeds were stored in tap water (pea) and in citric acid pickle pH = 2.5 (chickpea) or pH = 2.0 (lentil, green gram) at 4-8 degrees C. The total microfilora increased during 21 days storage in pressurized germinated pea seeds to 7.5 x 10(4) CFU/g and in green gram to 3.5 x 10(5). The contents of microorganisms in pressurized germinated chickpea and lentil seeds were under detection limits of methods used.

The α-galactosides negatively affect of digestibility of grain legumes. The most effective way of α-galactosides decreasing is germination. The content of α-galactosides in chick-pea was decreased by 4 days germination up to 24% of original value in dry seeds. The 2 days germinated chick-pea seeds were treated by pressure 500 MPa for 10 minutes. The α-galactosides content was decreased by this treatment up to 31% of value before pressurisation and during 21 days storing decreased up to 7% of value before pressurisation.