The present study recorded the seroprevalence of Mycoplasma in sheep and goats of Himachal Pradesh based on agglutination test using Mycoplasma mycoides subsp. capri colored antigen. Out of the 314 serum samples screened, 15 (4.77 %) were found positive for mycoplasmosis. The prevalence was found to be slightly higher in goats (5.02 %) compared to sheep (4.44 %). The seroprevalence of mycoplasmosis was the highest in Kangra district (11.59 %) followed by Mandi district (9.67 %) and Hamirpur district (2.17 %). The high seroprevalence of mycoplasmosis in Kangra district may be attributed to the migratory flocks as the district falls along their route of migration followed by the nomadic tribes on their way to the higher reaches of Kullu, Mandi and Chamba in summers and back to their respective grazing areas in the lower hills and plains during winters.