Site-selective laser spectroscopy has been used to resolve the spectral features of lanthanide fluorescence probe ions in calcium-binding proteins. The capabilities and characteristics of this technique are studied using bovine brain calmodulin where the calcium-binding sites are very similar. Two distinct spectral features are identified. These features were followed during a Eu3+ titration and were found to fill successively, showing they correspond to the high- and low-affinity sites. One set of spectral features is assigned to domains I and III, which are the high-affinity domains, while the other set is assigned to domains II and IV. Additional nonspecific binding is observed after the domains are filled. Tb3+ titrations confirmed earlier results that the tyrosine-containing domains fill second and third (R. W. Wallace, E. A. Tallant, M. E. Duckter, and W. Y. Cheung, 1982, J. Biol. Chem.X257(4), 1845–1854). Site-selective laser spectroscopy was also used to identify the presence of ethylene glycol bis(β-aminoethyl ether) N,N′-tetraacetic acid contamination that could cause interference in titrations.

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPrinciples of LasersJ. C. Wright and M. J. WirthCite this: Anal. Chem. 1980, 52, 9, 1087A–1095APublication Date (Print):August 1, 1980Publication History Published online31 May 2012Published inissue 1 August 1980https://pubs.acs.org/doi/10.1021/ac50059a780https://doi.org/10.1021/ac50059a780research-articleACS PublicationsRequest reuse permissionsArticle Views248Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLasers and SpectroscopyJ. C. Wright and M. J. WirthCite this: Anal. Chem. 1980, 52, 9, 988A–996APublication Date (Print):August 1, 1980Publication History Published online31 May 2012Published inissue 1 August 1980https://pubs.acs.org/doi/10.1021/ac50059a715https://doi.org/10.1021/ac50059a715research-articleACS PublicationsRequest reuse permissionsArticle Views118Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts