ADVERSE reactions to cow's milk have been estimated to occur in 0.1 to 7.5 percent of children.1 , 2 A variety of manifestations have been reported, including urticaria, angioedema, atopic dermatitis, rhinitis, wheezing, anaphylactic reactions, various gastrointestinal syndromes, and failure to thrive.3 , 4 Some patients may be extremely sensitive to milk; the ingestion of as little as one drop has been reported to cause generalized reactions.5 In children, repeated exposures to small quantities of food allergens may lead to recurrence of symptoms and delay the resolution of food allergy.2 , 6 , 7 The management of sensitivity to cow's milk is based on the complete elimination of . . .

A novel nucleic acid detection technique is described for the quantitative measurement of eukaryotic mRNA in biological samples. The procedure involves two steps: a hybridization reaction in solution with a biotinylated cDNA probe, and a conventional enzyme immunoassay that uses a monoclonal antibody for DNA.RNA hybrids to detect the specific mRNA.cDNA complexes. The method has comparable sensitivity to 32P-based methods and yields results that are quantitative and highly reproducible. Furthermore, the test can be performed using unfractionated cytoplasm without the need for extraction with organic solvents. This technique provides a rapid and quantitative method for studying changes in cellular mRNA levels, and it is suitable for testing large numbers of samples.