Speleothems, such as stalagmites and flowstones, are invaluable archives of past environmental and climatic conditions due to their layered growth and suitability for precise 230Th/U dating. In tropical karst settings, however, elevated and variable detrital thorium contamination can severely compromise age accuracy, especially for young speleothems. Here, a suite of stalagmites from Áaktun Kóopo' Cave from the Yucatán Peninsula is used as a case study to develop and evaluate a multi-method framework for constraining initial thorium and constructing robust age–depth models. We combine extensive 230Th/U dating with local isochron analysis, stratigraphic approaches, and annual Sr/Ca layer counting to constrain elevated and initial (230Th/232Th) activity ratios and resolve pronounced chronological inversions. High uranium concentrations in the speleothems (average ca. 1 ppm) allow precise measurements, yet we infer a high and largely unsystematic variability of high initial (230Th/232Th) activity ratios in space and time, with values spanning between 4 and 68, thus far exceeding standard bulk earth values. This variability demonstrates that elevated and temporally variable initial Th may be common in tropical karst settings and that multiple, independent constraints on initial (230Th/232Th) activity ratios are essential for reliable chronologies in such environments. Applying this framework to Áaktun Kóopo' Cave stalagmites yields internally consistent stalagmite age–depth models spanning the past 2.7 kyr, as well as evidence for earlier glacial and interglacial growth phases. These chronologies provide the basis for high-resolution, multi-proxy reconstructions of climatic and environmental changes in the northeastern Yucatán Peninsula during the entire era of Maya cultural evolution, and they illustrate how robust speleothem chronologies can be obtained even in cave systems affected by elevated and highly variable initial thorium.

Hells Bells are underwater secondary carbonates discovered in sinkholes (cenotes) southeast of Cancun on the north-eastern Yucatán peninsula, Mexico. These authigenic calcite precipitates, reaching a length of up to 4 m, most likely grow in the pelagic redoxcline. Here we report on detailed 230 Th/U-dating and in-depth geochemical and stable isotope analyses of specimens from cenotes El Zapote, Maravilla and Tortugas. Hells Bells developed since at least ~ 8000 years ago, with active growth until present day. Initial ( 234 U/ 238 U) activity ratios (δ 234 U 0 ) in Hells Bells calcite decreas from 55 to 15‰ as sea level converges toward its present state. The temporal evolution of the geochemistry and isotope composition of Hells Bells calcites thus appears to be closely linked to sea-level rise and reflects changing hydrological conditions (desalinization) of the aquifer. We suggest that decelerated leaching of excess 234 U from the previously unsaturated bedrock traces Holocene relative sea-level rise. Considering this proxy, the resulting mean sea-level reconstruction contains half as much scatter, i.e. improves by a factor of two, when compared to previously published work for the period between 8 and 4 ky BP.

Hells Bells are underwater secondary carbonates discovered in sinkholes (cenotes) southeast of Cancun on the north-eastern Yucatán Peninsula, Mexico. These authigenic calcite precipitates, reaching a length of up to 4 m, most likely grow in the pelagic redoxcline. Here, we report on detailed 230Th/U-dating and in-depth geochemical and stable isotope analyses of specimens from cenotes El Zapote, Maravilla and Tortugas. Hells Bells developed during the end of MIS5b/c (~96–90 thousand years ago) and again since the early Holocene, with active growth until present day. The temporal evolution of the geochemistry and isotope composition of Hells Bells calcites appears closely linked to the mid to late Holocene sea-level rise, which reflects changing hydrological conditions of the aquifer. A stabilization of sea level combined with aquifer occlusion during the past ~8 thousand years probably led to a reduction in hydraulic conductivity and a desalinization of the freshwater layer as indicated by decreasing Sr/Ca values. In addition, initial (234U/238U) activity ratios (δ234U0) in the Bells calcite decrease from 55 to 15‰ as sea level converges toward its present state. We propose that the Holocene sea-level rise drives desalinization and subsequent deceleration of leaching of excess 234U from the previously unsaturated bedrock.

Recent palaeontological research in submerged caves of the north-eastern Yucatan Peninsula (YP) in Mexico has resulted in the identification of a diverse megafaunal assemblage in the area, among them Late Pleistocene ground sloths assigned to the family Megalonychidae (Xenarthra). Here we report on a new species of Megalonychidae,Xibalbaonyx exinferisn. sp., from a new fossil locality named cenote Tortugas, located west of Puerto Morelos in the federal state of Quintana Roo. The taxon is based on a fragmentary left mandibular ramus, an atlas, and a left humerus. The new taxon is diagnosed by the presence of two mental foramina on the short symphyseal spout, a caniniform being the smallest mandibular tooth, and the anterolaterally directed aperture of the mandibular foramen on the lateral surface of the mandible.Xibalbaonyx exinferisis the third endemic megalonychid documented for the north-eastern Yucatan Peninsula and thus provides increasing evidence for an ecological isolation of the area from the rest of Mexico during the Pleistocene.

ABSTRACTWe examined 14 subaerially deposited speleothems retrieved from submerged caves in the northeastern Yucatán Peninsula (Mexico). These speleothems grew during the Middle to Late Quaternary and were dated by 230Th‐U techniques to provide upper depth limits for past sea levels. We report the first relative sea‐level limits for Marine Isotope Stages (MIS) 11 and 6, and present new evidence for sea‐level oscillations during MIS 5 and early MIS 1. For the latter periods, the origin of growth interruptions is evaluated by combining petrographic methods with trace element analyses. The MIS 5c sea‐level highstand probably occurred between 103.94 ± 0.58 ka and 96.82 ± 0.42 ka and must have exceeded ‐10.8 m (relative to present‐day local sea level). The minimum average rate of sea‐level fall over a 9.4 ka‐long period during the MIS 5e/5d transition is calculated from stalagmite and published coral data at 1.74 ± 0.37 m/ka. For the early Holocene, previous discrepancies with respect to a potential multimetre oscillation of local sea level were found to be challenging to reconcile with the existing speleothem data from the area.

A speleothem record from the north-eastern Yucatán peninsula (Mexico) provides new insights into the tropical hydro-climate of the Americas between 11,040 and 9520 a BP on up to sub-decadal scale. Despite the complex atmospheric reorganization during the end of the last deglaciation, the dominant internal leading modes of precipitation variability during the late Holocene were also active during the time of record. While multi-decadal variations were not persistent, Mesoamerican precipitation was dominated by changes on the decadal- and centennial scale, which may be attributed to ENSO activity driven by solar forcing. Freshwater fluxes from the remnant Laurentide ice sheet into the Gulf of Mexico and the North Atlantic have additionally modulated the regional evaporation/precipitation balance. In particular, this study underlines the importance of solar activity on tropical and subtropical climate variability through forcing of the tropical Pacific, providing a plausible scenario for observed recurrent droughts on the decadal scale throughout the Holocene.

Schubert and colleagues have recently criticized our assessment of the mandibular ramus of a small peccary from Muknal cave in Quintana Roo, Mexico, to a new genus and species, 'Muknalia minima' Stinnesbeck et al. 2017. They considered this assignation as invalid and the unique morphologies of the taxon to be the result of breakage and human modification. We strongly disagree with this interpretation and maintain our original view of a new genus and species.

Ongoing investigations in submerged cave systems of Quintana Roo in south-eastern Mexico reveal a rich Late Pleistocene megafaunal assemblage, among them the megalonychid ground slothXibalbaonyx oviceps. The taxon has been described based on a complete skull and mandible from El Zapote cenote west of Puerto Morelos. We here add hitherto unreported postcranial material from El Zapote, attributed to the holotype. This new material allows us to reconstruct unexpected locomotion capabilities forXibalbaonyx ovicepsincluding steep slope and rock climbing. This may have enabled the ground sloth to use the sinkholes and underground caverns as water resource and shelter. The Late Pleistocene age of the fossil allows for a co-existence with early human settlers on the Yucatan Peninsula.

The unsteady cultural evolution and final collapse of Maya civilization in Mesoamerica are heavily debated issues and discussion includes the impact of both natural (e.g., droughts, hurricanes, volcanic eruptions) and social disasters (e.g., warfare and unsustainable economy). An increasing number of records point to recurrent multi-year droughts coinciding with hiatuses in construction, periods of temporary urban abandonment and population collapse. Previous reconstructions indicate that environmental conditions and precipitation on the Yucatán Peninsula were distributed very heterogeneously in space and time and the duration and chronology of events remains uncertain. High resolution environmental reconstructions are, however, mainly based on archives from sites on the southern Yucatán Peninsula. We have now recovered several stalagmites from Estrella Cave, northern Yucatán Peninsula, spanning the entire Maya era and reaching even to historical times (−1100 to 1780 AD). The high precision 230Th/U ages obtained so far from these stalagmites indicate growth rates of up to 160 µm per year, thus offering the potential for annual to decadal climate proxy reconstruction. Here we present 230Th/U based preliminary age models for some of these stalagmites. Based on growth rates, petrographic observations, and trace element to calcium ratios we draw first conclusions on the timing of (recurrent) dry periods around key episodes of Maya cultural evolution, such as the Terminal Classic Period (~800–1000 AD). Furthermore, these first results show that the incorporation of certain trace elements (Mg, Sr, Ba, P, …) in these speleothems is strongly related to recharge and hence precipitation above the cave. Moreover, the site also contains unique remains of the Mayan culture, such as paintings, pottery, constructions and even buried skeletons, thus highlighting its significance not only for regional climate reconstruction but also for local archaeology.

Ongoing investigations in submerged cave systems of Quintana Roo in south-eastern Mexico reveal a rich Late Pleistocene megafaunal assemblage, among them the megalonychid ground sloth Xibalbaonyx oviceps. The taxon has been described based on a complete skull and mandible from El Zapote cenote west of Puerto Morelos. We here add hitherto unreported postcranial material from El Zapote, attributed to the holotype. This new material allows us to reconstruct unexpected locomotion capabilities for Xibalbaonyx oviceps including steep slope and rock climbing. This may have enabled the ground sloth to use the sinkholes and underground caverns as water resource and shelter. The Late Pleistocene age of the fossil allows for a co-existence with early human settlers on the Yucatán Peninsula.

The human settlement of the Americas has been a topic of intense debate for centuries, and there is still no consensus on the tempo and mode of early human dispersion across the continent. When trying to explain the biological diversity of early groups across North, Central and South America, studies have defended a wide range of dispersion models that tend to oversimplify the diversity observed across the continent. In this study, we aim to contribute to this debate by exploring the cranial morphological affinities of four late Pleistocene/early Holocene specimens recovered from the caves of Quintana Roo, Mexico. The four specimens are among the earliest human remains known in the continent and permit the contextualization of biological diversity present during the initial millennia of human presence in the Americas. The specimens were compared to worldwide reference series through geometric morphometric analyses of 3D anatomical landmarks. Morphological data were analyzed through exploratory visual multivariate analyses and multivariate classification based on Mahalanobis distances. The results show very different patterns of morphological association for each Quintana Roo specimen, suggesting that the early populations of the region already shared a high degree of morphological diversity. This contrasts with previous studies of South American remains and opens the possibility that the initial populations of North America already had a high level of morphological diversity, which was reduced as populations dispersed into the southern continent. As such, the study of these rare remains illustrates that we are probably still underestimating the biological diversity of early Americans.

Human presence on the Yucatán Peninsula reaches back to the Late Pleistocene. Osteological evidence comes from submerged caves and sinkholes (cenotes) near Tulum in the Mexican state of Quintana Roo. Here we report on a new skeleton discovered by us in the Chan Hol underwater cave, dating to a minimum age of 9.9±0.1 ky BP based on 230Th/U-dating of flowstone overlying and encrusting human phalanges. This is the third Paleoindian human skeleton with mesocephalic cranial characteristics documented by us in the cave, of which a male individual named Chan Hol 2 described recently is one of the oldest human skeletons found on the American continent. The new discovery emphasizes the importance of the Chan Hol cave and other systems in the Tulum area for understanding the early peopling of the Americas. The new individual, here named Chan Hol 3, is a woman of about 30 years of age with three cranial traumas. There is also evidence for a possible trepanomal bacterial disease that caused severe alteration of the posterior parietal and occipital bones of the cranium. This is the first time that the presence of such disease is reported in a Paleoindian skeleton in the Americas. All ten early skeletons found so far in the submerged caves from the Yucatán Peninsula have mesocephalic cranial morphology, different to the dolicocephalic morphology for Paleoindians from Central Mexico with equivalent dates. This supports the presence of two morphologically different Paleoindian populations for Mexico, coexisting in different geographical areas during the Late Pleistocene-Early Holocene.

The loading of the North American continent with ice sheets causes a geomorphologic response. As a result of this process, a NW-SE gradient of relative sea level developed in the Caribbean during periods of glaciation. In order to distinguish geomorphologic and eustatic contributions it is important to resolve timing and amplitude of relative sea level at different positions in the Caribbean. The cave systems around Tulum, Quintana Roo, Mexico are presently submerged and well-connected to the nearby Atlantic with a low hydraulic head gradient. Speleothems must have formed during periods of lower sea level, thus providing constraints on the maximum elevation of relative sea level for given periods of time. Conversely, periods of growth cessation could have been caused by sea level rise thus indicating minimum relative sea level during highstands. Here, we present 230Th/U dated submerged speleothems that grew during MIS5a-d as well as MIS1/2, MIS6 and MIS11/12. Growth of a single stalagmite (QUE01) at -10.8±0.1m (relative to today's sea level) was interrupted twice. Petrographical studies and trace element analysis indicate that submergence caused millennial-scale growth stops in QUE01 during MIS5. The proposed highstands are between 109.4±0.3ka and 105.0±0.3ka as well as between 104.5±0.4ka and 96.9±0.4ka. While a previous study [1] constrains the amplitude to <9.9m, this study further improves the timing. This is the first record in this area that yields bracketing ages for those highstands from a speleothem that is very close to the peak height. In order to reconstruct a Caribbean sea level gradient, the combined Yucatán record acts as a counterpiece to a similar study from the northern end of the Caribbean sea level gradient which reports highstands at that time with a higher relative sea level [4]. Speleothem growth during MIS1/2 (19-8ka) relates to conflicting local sea level markers [2,3] and contains century-scale growth stops. Samples dating back to MIS6 and MIS11/12 highlight the potential for sea level reconstruction in this area before MIS5. [1] Moseley et al. (2013) Journal of Quaternary Science 28 293-300 [2] Moseley et al. (2015) The Holocene 25 1511-1521 [3] Hering et al. (2018) Journal of Quaternary Science 33 444-454 [4] Wainer et al. (2017) Earth and Planetary Science Letters 457 325-334

Unique bell-shaped underwater speleothems were recently reported from the deep (∼ 55 m) meromictic El Zapote sinkhole (cenote) on the Yucatán Peninsula, Mexico. The local diving community has termed these speleothems as Hells Bells because of their shape and appearance in a dark environment in ∼ 28–38 m water depth above a sulfidic halocline. It was also suggested that Hells Bells form under water, yet the mystery of their formation remained unresolved. Therefore, we conducted detailed hydrogeochemical and geochemical analyses of the water column and Hells Bells speleothems including stable carbon isotopes. Based on the comprehensive results presented in this study we deduce that both biogeochemical processes in the pelagic redoxcline and a dynamic halocline elevation of El Zapote cenote are essential for Hells Bells formation. Hells Bells most likely form in the redoxcline, a narrow 1–2 m thick water layer immediately above the halocline where a pelagic chemolithoautotrophic microbial community thrives from the upward diffusion of reduced carbon, nitrogen and sulfur species released from organic matter degradation in organic-rich debris. We hypothesize that chemolithoautotrophy, in particular proton-consuming nitrate-driven anaerobic sulfide oxidation, favors calcite precipitation in the redoxcline and hence Hells Bells formation. A dynamic elevation of the halocline as a hydraulic response to droughts, annual tidal variability and recharge events is further discussed, which might explain the shape of Hells Bells as well as their occurrence over a range of 10 m water depth. Finally, we infer that highly stagnant conditions, i.e., a thick halocline, a low-light environment and sufficient input of organic material into a deep meromictic cenote are apparent prerequisites for Hells Bells formation. This might explain their exclusivity to only a few cenotes in a restricted area of the northeastern Yucatán Peninsula.

Here we describe a new species of a Pleistocene felid based on the distal third of a right humerus from the submerged El Pit cenote (sinkhole) near Tulum in Quintana Roo, Mexico. The new taxon, Panthera balamoides sp. nov., is characterized by a large entepicondylar foramen, a gracile and straight humeral shaft with a prominent supracondylar ridge with a small depression on the lateral epicondyle and a distal articular surface located medially with respect to the long axis of the shaft.Two felid clavicles from the same locality have been assigned to Panthera atrox, while a humerus fragment from the Kim Ha cave near Tulum likely corresponds to Smilodon gracilis. Panthera balamoides lines up with other likely endemic mammals in the region, which suggest that at least northern Quintana Roo, if not the entire Yucatán peninsula, may have been ecologically isolated during the Pleistocene, due to the repeated expansion of grassland.

Here, we report on an incomplete human skeleton, soot patches related to anthropogenic fireplaces, and cut marks on the mandible of an extinct peccary, from the submerged Muknal cave southwest of Tulum on the Mexican Yucatán peninsula. The human individual, here named ‘Muknal Grandfather’, was identified as a male based on cranial parameters. The age at the time of death was estimated to be between 40 and 45 years. We propose that the human bones have been brought to the cave during the latest Pleistocene or early Holocene, but not later than 8600 14C yr BP (ca. 9600 cal BP), as a secondary burial of a partial skeleton. The peccary mandible was placed close to the burial site, possibly as part of the same ritual. The Muknal cave therefore served as a place for funeral rituals.

We here report on a type of meter-sized pendant speleothem growing under water in the submerged El Zapote sinkhole (cenote) west of Puerto Morelos on the Mexican Yucatán Peninsula. These conical, mantle-shaped downward expanding and diverging calcareous structures, here termed as Hells Bells, are yet unreported in the scientific literature. They are characterized by bell- or trumpet shaped longitudinal and circular, elliptical or horse-shoe-like horizontal cross-sections. Hells Bells grow downward, based on the downward divergence of the structures and the horizontally laminated internal texture of both blade-shaped spar calcite and microspar laminae. Age dating confirms that Hells Bells are young (<4500yr) and formed in a subaquatic environment. They grow under lightless conditions in a stratified water body, which is characterized by a fresh water body overlying a salt water body with a stagnant transition zone (halocline) of several meters. We hypothesize that the growth of these structures is mediated by specific physical and biogeochemical conditions above and in the halocline. Stagnant hydraulic conditions led to extensive diffusion profiles of several nutrients including calcium originating from the salt water body. Dissolved organic carbon from the fresh water is microbially oxidized in the upper part of the halocline, where a distinct redox zonation was identified from oxic to anoxic conditions. Degradation processes combined with slightly alkaline pH values as well as the diffusive transport of calcium into this zone may induce an increase in calcite oversaturation. Phylogenetic analysis of the community on the surface of the Hells Bells suggests the presence of microorganisms involved in the nitrogen-cycle, from which some potentially have the capability to increase the pH by autotrophic growth and denitrifying activity, thus supporting calcite precipitation. The growth of Hells Bells is strictly dependent on the elevation of the halocline. This offers a wide potential for the use of Hells Bells as archives of paleo-hydrological conditions during the Holocene, e.g. the variation of thickness of the fresh water lens on the Yucatán Peninsula.

ABSTRACTNumerous charcoal accumulations discovered in the submerged Chan Hol cave near Tulum, Quintana Roo, Mexico, have been 14C‐dated revealing ages between 8110 ± 28 14C a BP (9122–8999 cal a BP) and 7177 ± 27 14C a BP (8027–7951 cal a BP). These charcoal concentrations, interpreted here as ancient illumination sites, provide strong evidence that the Chan Hol cave was dry and accessible during that time interval. Humans used the cave for at least 1200 years during the early and middle Holocene, before access was successively interrupted by global sea level rise and flooding of the cave system. Our data thus narrow the gap between an early settlement in the Tulum area reaching from the late Pleistocene (∼13 000 a) to middle Holocene (e.g. 7177 14C a BP), and the Maya Formative period at approximately 3000 a bp. Yet, no evidence has been presented to date for human settlement during the ∼4000‐year interval between 7000 and 3000 a. This is remarkable as settlement in other areas of south‐eastern Mexico (e.g. Chiapas, Tabasco) and in Guatemala was apparently continuous.

Preceramic human skeletal remains preserved in submerged caves near Tulum in the Mexican state of Quintana Roo, Mexico, reveal conflicting results regarding 14C dating. Here we use U-series techniques for dating a stalagmite overgrowing the pelvis of a human skeleton discovered in the submerged Chan Hol cave. The oldest closed system U/Th age comes from around 21 mm above the pelvis defining the terminus ante quem for the pelvis to 11311±370 y BP. However, the skeleton might be considerable older, probably as old as 13 ky BP as indicated by the speleothem stable isotope data. The Chan Hol individual confirms a late Pleistocene settling of Mesoamerica and represents one of the oldest human osteological remains in America.