The collagen I was made with the dialysis method of enzymolysising the pig skin, and the static deposition in vitro of calcium phosphate was comparative studied by X-ray diffraction (XRD) and infrared spectroscopy (FTIR) under the condition of pH7. 4, Ca/P 1.67 and whether adding the collagen I into the system. Then the chemical composition of the sedimentary product and the diversification of the collagen I 's IR and Raman spectra (RS) before and after the mineralization were analyzed. The results showed that,under the physiological pH condition that there was not any collagen I, though Ca/P reached up to 1.67, the sedimentary product was CaHPO4 x 2H2O yet, however, after adding collagen I into the system, the bone-like apatite was deposited, which proved that collagen I indeed had the effects on the inducing of the bone-like apatite's mineralization in vitro; there was obviously mutual coordination action between collagen I and its mineralization product--bone-like apatite, which caused that amide peak I red-shifted, amide peak II and amide peak III decreased significantly or disappeared on the IR of collagen I, which maybe was the mechanism that how collagen I induced the depositing of the bone-like apatite.
用酶解猪皮透析法自制Ⅰ型胶原,利用X射线衍射(XRD)和红外吸收光谱(FTIR)对比研究了在pH7 4、Ca/P1 67和有无Ⅰ型胶原条件下磷酸钙的体外静态沉积,分析了沉积产物的化学组成和矿化前后Ⅰ型胶原红外光谱和拉曼光谱的变化.结果表明,在无胶原的生理pH条件下,即便Ca/P高达1 67,沉积产物仍是CaHPO4·2H2O,而在有胶原时则沉积出类骨HA,证实Ⅰ型胶原确有体外诱导沉积类骨HA的矿化作用;胶原与其矿化产物类骨HA之间存在明显的配位相互作用,致使Ⅰ型胶原红外谱图中酰胺Ⅰ峰红移、酰胺Ⅱ峰与酰胺Ⅲ峰大幅减弱或消失,这是胶原诱导沉积类骨HA的可能机制.
UV-curing organosilicone acrylate was synthesized with DMC and acrylic acid as raw materi-als.The effects of polymerization inhibitor and water-carrying agent on the synthesis were studied.The me-chanical properties,surface energy,curing time and solubility of the UV-curing membrane were deter-mined,and the UV-curing membrane was characterized by infrared spectroscopy(FTIR).The mechanical properties of the UV-curing membrane are good and surface energy is low while it is adjusted by adding different monomers.The UV-curing membrane is insoluble in water and acid solution,but soluble in or-ganic solvents and alkaline solution.
