Objective:To establish a LC/MS method for the determination of Misoprostol in its tablet.Methods:The separation was performed on a waters symmetry C18(2.1mm×150mm,5μm) column with the column temperature 20℃.The mobile phase was acetonitrile-water(70:30) at a flow rate of 0.2mL/min.The selected ion mass spectral(M/Z)383(Misoprostol,M+H) was used for quantitation.Results:The lower limit of qualification was 1μg·mL.The calibration curve was in good linearity over the range of 1~5μg·mL(r=0.9998).The intra-day and inter-day RSD showed less than 1.2 %.Conclusion:LC/MS is sensitive,rapid and suitable for the determination of Misoprostol in its tablet.

目的:开展光果葶苈的解剖学研究,为进一步显微鉴别提供依据,也为细胞学研究提供基础材料.方法:运用徒手切片法,撕剥法及水合氯醛透化法对光果葶苈的营养器官和生殖器官进行了显微观察.结果:光果葶苈的根部有次生构造,木质部射线较韧皮部射线宽广;茎中维管束有10余个,大小各异,髓部宽广;叶片表皮气孔多为不等式,叶脉维管束的下方有分泌道;花瓣上有油室,可见小油滴.花粉粒圆球形,表面有粗细不等的刺状突起.结论:对黑龙江省光果葶苈各部分的构造特点有一个清晰的认识,为显微鉴定和细胞学研究提供科学依据.