OBJECTIVE:To study the anti-tumor effect of Compound lingzhi capsules. METHODS:To observe the inhibition effect of Compound lingzhi capsules(9.00,4.50,2.25 g·kg-1) on 3 kinds of tumor cells (S180 sarcoma cells, H22 liver cancer cells and Lewis lung cells), the inhibition effect of cyclophosphamide (CTX) and 5-fluorouracil (5-Fu)with Compound lingzhi capsules on S180 sarcoma cells. RESULTS: Compound lingzhi capsules 9.00, 4.50 and 2.25 g·kg-1 had certain inhibition effect on the growth of 3 kinds of tumor cells. Compound Lingzhi capsules 9.00,4.50 and 2.25 g·kg-1 cooperated with CTX or 5-Fu had stronger inhibition effect on the growth of S180 than 0.01 g·kg-1 CTX or 5-Fu alone. CONCLUSION: Compound lingzhi capsules have certain inhibition effect on the growth of 3 kinds of tumor cells, and have ability to cooperate with chemotherapy drug.

Objective: To use p-toluene sulfonic acid as the catalyst for the esterification reaction of phenylformic acid with menthol in liquid phase.Methods:We explore the effects of the molar ratio of propanolto acid,the reaction time and the amount of the catalyst used on the synthesis of L-menthyl benzoate to obtain a suitable condition for synthesizing the ester.Results:The optimum reaction conditions showed that the reaction time was 8.0h,the molar ratio of propanolto acid was 1.0:1.4 and the mass ratio of the catalyst used to the reactants was 3.0%,and under this conditions the yield of propy1benzoate reached 80.0%.Conclusion:p-toluene sulfonicacid is an excellent catalyst for synthesizing L-menthyl benzoate,and the technical process is simple,with applied value and higher yield.

目的:观察黄药子对人甲状腺癌细胞株SW579细胞凋亡抑制蛋白Survivin的诱导作用,探讨黄药子抑制甲状腺癌细胞增殖的作用机制。方法:48只成年雄性Wistar大鼠分为空白对照组和药物高、中、低剂量组,空白对照组给予生理盐水,药物组给予不同剂量的黄药子(100、200、400mg/kg),15天后,分别提取其血清,对SW579细胞进行处理,采用RT-PCR和Western blotting方法分别检测Survivin mRNA和蛋白的表达。结果:中、高剂量药物组细胞Survivin mRNA表达明显低于空白对照组(P<0.01);中、高剂量药物组细胞Survivin的蛋白表达明显低于空白对照组(P<0.01)。结论:黄药子能明显下调人甲状腺癌细胞株SW579 Survivin mRNA和蛋白的表达,诱导其细胞凋亡。

目的:研究单用黄药子和黄药子配伍甘草后对大鼠肝组织CYP3A1、CYP2E1 mRNA表达的影响。方法:采用实时定量反转录-聚合酶链反应（Quantitive realtime RT-PCR）定量分析大鼠给药后CYP450亚型CYP3A1、CYP2E1 mRNA表达水平变化。结果:黄药子可明显诱导CYP3A1、CYP2E1 mRNA表达,黄药子配伍甘草后该表达受到抑制。结论:甘草可抑制黄药子引起的大鼠肝脏组织CYP3A1和CYP2E1表达,有减轻其毒性作用。

Objective:To establish a LC/MS method for the determination of Misoprostol in its tablet.Methods:The separation was performed on a waters symmetry C18(2.1mm×150mm,5μm) column with the column temperature 20℃.The mobile phase was acetonitrile-water(70:30) at a flow rate of 0.2mL/min.The selected ion mass spectral(M/Z)383(Misoprostol,M+H) was used for quantitation.Results:The lower limit of qualification was 1μg·mL.The calibration curve was in good linearity over the range of 1~5μg·mL(r=0.9998).The intra-day and inter-day RSD showed less than 1.2 %.Conclusion:LC/MS is sensitive,rapid and suitable for the determination of Misoprostol in its tablet.

目的比较庆大霉素和利福平高渗盐水对烧伤肉芽创面治疗效果。方法将112例烧伤肉芽感染创面患者,随机分为A、B两组。A组43例,采用庆大霉素高渗盐水纱布处理创面;B组69例采用利福平高渗盐水纱布处理创面。比较两组治疗前后病原菌检出率及烧伤肉芽创面植皮后成活率。结果治疗后A组病原菌检出率明显高于B组(P<0.05)。A组植薄中厚皮,全部成活29例,皮片成活面积在96%以上14例,散在1cm×1cm残余创面≤3个;B组植薄中厚皮,全部成活63例,皮片成活面积在96%以上6例,散在1cm×1cm残余创面≤3个。两组比较差异有显著意义(P<0.05)。结论利福平高渗盐水纱布湿敷创面优于庆大霉素高渗盐水纱布处理创面,具有高效、省时的特点,易于在临床上推广。

目的总结和探讨透明质酸钠在手外伤肌腱断裂吻合术中的临床效果。方法自2002～2003年,我们对88例手外伤术后肌腱损伤修复处的鞘内或局部注射透明质酸钠预防肌腱粘连,取得良好效果。结果88例患者中8例功能活动优良,无感染发生,无肌腱粘连。结论透明质酸钠预防肌腱粘连效果良好且无明显毒副作用。

肿瘤疾病已经成为威胁人类健康和生命的主要敌人之一,其发病率和死亡率都在逐年上升.死亡率仅次于心血管病,是人类的第二大杀手.如何通过有效措施防治恶性肿瘤已成为我国以至全世界防治疾病的首要任务,也是全社会特别是医学领域急待解决的重大课题.但由于癌症早期诊断的困难,相当数量的癌症患者将依赖药物治疗,因此,抗肿瘤药物的研发与应用是生物医药科学迅速发展的一个重要领域.

目的:观察黄药子水煎浓缩液的氯仿、乙酸乙酯、正丁醇三个不同分离部位对大鼠肝脏的毒性生化指标及组织病理变化,明确黄药子的毒性部位。方法:比较黄药子三个不同分离部位对大鼠体重、肝脏指数、GPT、GOT及病理变化,观察黄药子不同分离部位是否产生毒性及毒性的差异。结果:黄药子的氯仿和乙酸乙酯分离部位可使大鼠体重减轻,肝脏指数、GPT、GOT明显增高,病理变化可见肝细胞疏松、肿胀、胞核溶解、融合、坏死、汇管区炎细胞浸润,小胆管水肿、增生等;正丁醇层对大鼠肝脏体重、肝脏指数、GPT、GOT及病理与正常组比较没有明显的差异。结论:黄药子毒性部位是氯仿和乙酸乙酯层。

目的建立高效液相色谱-蒸发光散射检测法测定黄芪颗粒中黄芪甲苷的含量。方法色谱柱:Kromasil C18柱(4.6×200mm,5μm);流动相:乙腈-水(40∶60);流速:1.0ml·min-1;柱温:40℃;ELSD参数:漂移管温度:105℃;载气流量:2.7L·min-1;放大系数:1;撞击器:Off。结果黄芪甲苷在1.24~5.86μg范围内呈线性,回收率为100.4%(RSD为0.91%,n=5)。结论本法具有良好的精密度和重现性,结果准确可靠,可作为本产品的质量控制方法。

Object:To develop a method for the determination of ferulic acid and Diosbulbin B in Dioscorea bulbifera L.and Angelica sinensis Diels decoction,and analyze the contents of these two compounds in co-boiling and pooled in-dividable boiling extracts.Method:An HPLC/PDA method was applied with KromasilC18 column.Gradient elute was performed by the mobile phrase consisted of 0.1 % acetic acid and color spectrum methanol with the flow rate of 0.8 mL·min-1.The column temperature was set at 30℃ and the detection was set at UV 300 nm,the sample injection volume was 10 μL.Results:The recovery of the method was in the range of 93.7%～102.8%,and all the compounds showed good linearity(r0.9995) in a detected concentration range.The contents of two compounds were different in co-boiling and pooled individual boiling extracts.Conclusion:The contents of ferulic acid increase and toxic Diosbulbin B decrease significantly after boiling the individual crude drugs together.

Objective To study the effect of concoction quality of Zhiqiao.Methods HPLC method was applied to determination of total flavone in Zhiqiao before and after concoct.Results The contents of total flavone in Zhiqiao and concoct were 8.615% and 7.542%,respectively.Conclusion Total flavone in Zhiqiao will decline after being concocted by wheat bran.

风湿病是一类常见病,近几年对抗风湿药的研究也是医药研究的重点.由于西药多具毒副作用,中药虽毒副作用小,但用量较大,活性成分又不清楚,因此开发一种疗效确切,毒副作用小的抗风湿药物,具有相当的紧迫性.

Objective:Reversed-phase high performance liquid chromatography(RP-HPLC) fingerprint of Dioscorea bulbifera L.Grown in Hunan Province was established.Methods:Chromatographic conditions were available as follows:Kromasil C18 column(250mm×4.6mm,5μm),methanol and water including 0.1% acetic acid as mobile phase,flow rate 0.8mL·min-1,gradient elution.The column temperature was set at 30℃ and detecting wavelength at 280nm.Results:The relative peak areas and retention time of common peak in precision,repeatability,and stability has not reached 5.0%,respectively Similarity was over 98.5 % in Dioscorea bulbifera L.collected from different habits.Conclusion:All result s above exhibited that this method is simple,practicable and reliable as a foundation method in controlling the quality of Dioscorea bulbifera L.and chromatographic fingerprint in vitro.