Acute kidney injury (AKI) represents a prevalent and complex clinical event, characterized by irreversible damage to renal tubular epithelial cells and high intensive care unit (ICU) admission rates and mortality. The kidneys are highly susceptible to oxidative stress, inflammation, pyroptosis, and programmed cell death. Pyroptosis poses a significant risk, exacerbating the damage and inflammation of renal tubular cells. Disulfiram (DSF), an FDA-approved medication for alcohol cessation, inhibits the pyroptotic pore-forming protein Gasdermin-D (GSDMD), positioning it as a potential solution for emergency relief against an inflammatory response. However, current obstacles include poor water solubility, rapid metabolism, and off-target effects. Inspired by this discovery, bovine serum albumin (BSA), which has already entered clinical application, has been utilized to produce safe and long-lasting nanoparticles (BSA@DSF NPs), addressing the challenges posed by DSF's physicochemical properties. By targeting the GSDMD protein, the potent pro-inflammatory effects of pyroptosis were mitigated, leading to the alleviation of AKI induced by ischemia-reperfusion injury. This research offers a straightforward and efficient concept for treating AKI, potentially enhancing the transition to clinical practice.

Multiple myeloma (MM) is an incurable malignancy of clonal plasma cells, characterized by high relapse rates and rapid development of drug resistance. The emergence of proteasome inhibitors has dramatically improved the therapeutic effect of MM; however, side effects and drug resistance still negatively affect the survival rate of MM. Nano-medicine has become a promising field for therapeutic innovation owing to its biodegradability and biocompatibility. Nanoparticles (NPs), when combined with MM therapeutic drugs, can reduce side effects, increase treatment efficacy, and alleviate drug resistance, providing a new direction for the treatment of MM. Restructuring drugs with NPs presents an ideal strategy for ongoing studies aimed at more effective therapies. Additionally, clinical nanomedicine research has yielded new opportunities for MM treatment. This review, guided by the development of MM therapeutic drugs, summarizes the past 20 years of research progress and breakthroughs in NP-based systems for treating MM and improving drug targeting ability.

ABSTRACT: Currently, radio frequency (RF) heating is emerging as an alternative to steam thermal recovery methods, owing to its numerous advantages including cleanliness, environmental friendliness, and cost-effectiveness. Achieving maximum heating distance and temperature during RF heating hinges on understanding the interaction between RF electromagnetic waves and rocks. To address this, the present study elucidates the RF heating mechanism, establishes geometric and mathematical models accounting for rock properties, and validates the mathematical model using laboratory experiments. The calculations reveal that lower specific heat, thermal conductivity, relative permittivity, and density of rocks, coupled with higher rock electrical conductivity, contribute to higher maximum reservoir temperatures. Additionally, the maximum heating distance increases with decreasing specific heat and density of rocks. Optimal values of thermal conductivity, relative permittivity, and electrical conductivity exist, maximizing the heating distance. These findings provide crucial guidance for optimizing rock properties, enhancing maximum heating temperature, and extending heating distances before implementing RF heating technology. 1. INTRODUCTION Heavy oil resources are abundant around the world and have broad development and utilization prospects. However, the characteristics of heavy oil with high viscosity and poor fluidity greatly increase the difficulty of extraction. Currently, the steam recovery technologies are widely used for heavy oil extraction, but it has gradually exposed many shortcomings such as high energy consumption, high carbon emissions, and difficulty in effectively exploiting low permeability, deep and thin layers of heavy oil. So many oil companies have no option but to develop new heavy oil mining technologies. A novel heavy oil extraction technology combined with electromagnetic power appears and is collectively referred to as "RF heating" by some oil companies and scholars. Some literatures (Godard and Rey-Bethbeder; Hu and Li et al.; Saeedfar and Lawton et al.; Bera and Babadagli, 2015; Ghannadi and Irani et al., 2016) have introduced its advantages, such as being environmentally friendly, highly heating efficiency, extracting resource in deep oil-bearing formation, avoiding extra heat loss and so on. In the early days, some scholars done some important researches of RF heating for improving heavy oil recovery. In recent years, there were some latest studies on the development of RF heating. world-renowned companies have conducted several researches on RF heating technology. The most representative one is a project called "ESEIEH" carried out in the Steepbank mine in Canada in 2012. Several antennas that emit RF electromagnetic waves are mainly used to radiate electromagnetic waves to reduce the viscosity of heavy oil (Rassenfoss, 2012). In this project, the first phase of field testing has been successfully completed. Besides, Bientinesi et al. conducted an RF heating experiment and proved that RF heating can effectively reduce the heavy oil viscosity (Bientinesi and Petarca et al., 2013). In 2017, Bera and Babadagli experimentally confirmed that Ni and Fe nanoparticles added to the heavy mixture can improve the heating efficiency of RF electromagnetic waves (Bera and Babadagli, 2017). Harris Company developed the "Heatwave" technology to exploit the abundant heavy oil and oil sand resources in Canada, and the field test was successful. In the test, an antenna was uses to radiate electromagnetic waves with the frequency of 6.78MHz into the reservoir, and the maximum heating distance reaches 12.5m (Wise and Patterson, 2016). Wang et al. studied the RF heating mode based on the antenna arrays in 2018 (Wang and Gao et al., 2018; Wang and Gao et al., 2018). The simulation results showed that the antenna array configuration can greatly increase the heating range of heavy oil reservoirs (Wang and Gao et al., 2019; Wang and Gao et al., 2020). However, based on the current research progress, there is a lack of research on the influence of the rock properties on the maximum temperature distribution and furthest heating distance, making it difficult to accurately predict the heating range and heating temperature.

ABSTRACT: To achieve precise heating of reservoir layers with varying thicknesses using the RF (radio frequency) heating method while minimizing thermal energy loss, a novel solution is proposed leveraging a multi-antenna configuration. In this configuration, consisting of two antenna elements, the application of equal and distinct voltages to the antenna elements is categorized based on the diverse characteristics of the reservoir. Additionally, the impact of inter-antenna element distances on temperature profiles was thoroughly examined within these configurations. Research results indicate that these configurations can effectively address varying heat requirements of the reservoir, whether necessitating more, less, or no additional heat, through the adjustment of both distance and voltage. This comprehensive approach offers a viable solution to enhancing the efficiency of heavy oil reservoir heating processes during RF heating in horizontal wells at the operational level. 1. INTRODUCTION As one of non-conventional hydrocarbon resources, heavy oil reservoirs are widely distributed in the world. Nevertheless, high viscosity prevents the rich resources from being large-scale exploited (Hassanzadeh and Harding, 2016). Besides, with the situation of sustained low oil prices, traditional thermal recovery projects have been terminated because of high production costs and low recovery efficiency (Hasanvand and Golparvar, 2014; Wise and Patterson, 2016). To solve the above problems, oil companies are exploring other alternative heating processes, one of the most promising being radio frequency (RF) heating technology. It is effective in deep, shallow, thin and thick reservoirs, besides the extra energy loss in the pipeline, wellbore and overburden can be avoided (Chhetri and Islam, 2008; Wang et al., 2018). In recent years, many scholars have investigated the feasibility of RF heating. Ramcharan et al., 2016 explored the viability of using RF heating to extract heavy oil by applying COMSOL Multiphysics and CMG software. Their research results indicated that when an operating frequency of 10 MHz and a current of 50 Amperes were applied, the oil recovery rate was in the range 30-60%. Saeedfar et al., 2016 developed a synthetic homogenous reservoir model to conduct the simulation of RF heating method, and the results revealed that the method had the ability of highly efficient heating of oil sand reservoir.

The second near-infrared (NIR-II) theranostics offer new opportunities for precise disease phototheranostic due to the enhanced tissue penetration and higher maximum permissible exposure of NIR-II light. However, traditional regimens lacking effective NIR-II absorption and uncontrollable excited-state energy decay pathways often result in insufficient theranostic outcomes. Herein a phototheranostic nano-agent (PS-1 NPs) based on azulenyl squaraine derivatives with a strong NIR-II absorption band centered at 1092 nm is reported, allowing almost all absorbed excitation energy to dissipate through non-radiative decay pathways, leading to high photothermal conversion efficiency (90.98 %) and strong photoacoustic response. Both in vitro and in vivo photoacoustic/photothermal therapy results demonstrate enhanced deep tissue cancer theranostic performance of PS-1 NPs. Even in the 5 mm deep-seated tumor model, PS-1 NPs demonstrated a satisfactory anti-tumor effect in photoacoustic imaging-guided photothermal therapy. Moreover, for the human extracted tooth root canal infection model, the synergistic outcomes of the photothermal effect of PS-1 NPs and 0.5 % NaClO solution resulted in therapeutic efficacy comparable to the clinical gold standard irrigation agent 5.25 % NaClO, opening up possibilities for the expansion of NIR-II theranostic agents in oral medicine.

Although the curative effect of hematological malignancies has been improved in recent years, relapse or drug resistance of hematological malignancies will eventually recur. Furthermore, the microenvironment disorder is an important mechanism in the pathogenesis of hematological malignancies. Immunogenic cell death (ICD) is a unique mechanism of regulated cell death (RCD) that triggers an intact antigen-specific adaptive immune response by firing a set of danger signals or damage-associated molecular patterns (DAMPs), which is an immunotherapeutic modality with the potential for the treatment of hematological malignancies. This review summarizes the existing knowledge about the induction of ICD in hematological malignancies and the current research on combining ICD inducers with other treatment strategies for hematological malignancies.

Multifunctional phototheranostics that integrate several diagnostic and therapeutic strategies into one platform hold great promise for precision medicine. However, it is really difficult for one molecule to possess multimodality optical imaging and therapy properties that all functions are in the optimized mode because the absorbed photoenergy is fixed. Herein, a smart one-for-all nanoagent that the photophysical energy transformation processes can be facilely tuned by external light stimuli is developed for precise multifunctional image-guided therapy. A dithienylethene-based molecule is designed and synthesized because it has two light-switchable forms. In the ring-closed form, most of the absorbed energy dissipates via nonradiative thermal deactivation for photoacoustic (PA) imaging. In the ring-open form, the molecule possesses obvious aggregation-induced emission features with excellent fluorescence and photodynamic therapy properties. In vivo experiments demonstrate that preoperative PA and fluorescence imaging help to delineate tumors in a high-contrast manner, and intraoperative fluorescence imaging is able to sensitively detect tiny residual tumors. Furthermore, the nanoagent can induce immunogenic cell death to elicit antitumor immunity and significantly suppress solid tumors. This work develops a smart one-for-all agent that the photophysical energy transformation and related phototheranostic properties can be optimized by light-driven structure switch, which is promising for multifunctional biomedical applications.

Induction of immunogenic cell death (ICD) in tumor combined with immune checkpoint blockade (ICB) therapy is widely developed to improve the efficacy of cancer immunotherapy. However, the current ICD induced based on apoptosis, i.e., immunogenic apoptosis, is often restricted in immunogenicity owing to the inflammatory quenching that occurs early in apoptosis. Recently, pyroptosis is demonstrated to be a more efficient ICD form, i.e., immunogenic pyroptosis. The cell contents released during pyroptosis can powerfully activate tumor immunogenicity. Herein, first, it is demonstrated that lower doses of epigenetic drug decitabine can increase GSDME expression in prostate cancer (PCa) RM‐1 cells and successfully induce an apoptosis‐pyroptosis transition after photodynamic therapy (PDT). Subsequently, a microenvironment dual‐responsive nano‐drug equipped with PD‐L1 blocking peptide (TSD@LSN‐D) is developed for self‐synergistic cancer immunotherapy. The poorly immunogenic RM‐1 PCa model confirm that the powerful antitumor immune response evoked by TSD@LSN‐D not only can effectively inhibit the primary tumor but also form a long‐term immune memory to prevent PCa recurrence and metastasis. To the best of authors’ knowledge, this work presents the first concept that promotes the apoptosis–pyroptosis transition after tumor PDT through epigenetic modulation. Furthermore, the powerful combination of immunogenic pyroptosis with ICB opens a new platform for PCa immunotherapy.

Accurate and rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is significant for early tracing, isolation, and treatment of infected individuals, which will efficiently prevent large-scale transmission of coronavirus disease 2019 (COVID-19). Here, two kinds of test strips for receptor binding domain (RBD) and N antigens of SARS-CoV-2 are established with high sensitivity and specificity, in which AIE luminogens (AIE-gens) are utilized as reporters. Because of the high brightness and resistance to quenching in aqueous solution, the limit of detection can be as low as 6.9 ng/mL for RBD protein and 7.2 ng/mL for N protein. As an antigen collector, an N95 mask equipped with a test strip with an excellent enrichment effect would efficiently simplify the sampling procedures. Compared with a test strip based on Au nanoparticles or fluorescein isothiocyanate (FITC), the AIEgen-based test strip shows high anti-interference capacity in complex biosamples. Therefore, an AIEgen-based test strip assay could be built as a promising platform for emergency use during the pandemic.

The main obstacle of multiple myeloma (MM) therapy is the compromised immune microenvironment, which leads to MM relapses and extramedullary disease progression. In this study, a novel strategy is reported of enhanced immunogenic cell death (ICD) immunotherapy with aggregation-induced emission (AIE) photosensitizer-loaded bovine serum albumin (BSA) nanoparticles (referred as BSA/TPA-Erdn), which can activate T cells, convert the cold tumor to hot, and reverse T cell senescence to restore the immune microenvironment for MM treatment. Loading AIE photosensitizer into the hydrophobic domain of BSA proteins significantly immobilizes the molecular geometry, which massively increases reactive oxygen species (ROS) generation and elicits a promising ICD immune response. Employing a NOD-SCID IL-2receptor gamma null mice model with MM patients' monocytes, it is shown that BSA/TPA-Erdn can simulate human dentric cell maturation, activate functional T lymphocytes, and increase additional polarization and differentiation signals to deliver a promising immunotherapy performance. Intriguingly, for the first time, it is shown that BSA/TPA-Erdn can greatly reverse T cell senescence, a main challenge in treating MM. Additionally, BSA/TPA-Erdn can effectively recruit more functional T lymphocytes into MM tumor. As a consequence, BSA/TPA-Erdn restores MM immune microenvironment and shows the best MM tumor eradication performance, which shall pave new insights for MM treatment in clinical practices.

As a frontier imaging technique for biomedical applications, photoacoustic (PA) imaging has been developed rapidly. The development of new design strategies and excellent PA imaging reagents to boost PA conversion is eagerly desirable for high quality PA imaging but complicated to realize. Herein, we develop a new strategy in which PA imaging reagents with better properties can be easily optimized by polymerization. A series of new PA imaging reagents were designed and synthesized. The polymerization strategy can effectively promote the PA signal by specifically increasing the thermal-to-acoustic conversion efficiency. As these materials shared the same building units, the optimized effectiveness of polymerization strategy in terms of near-infrared light-harvesting capacity and thermal-to-acoustic conversion efficiency are discussed, rationally. The polymers with intense intramolecular motion exhibit an amplified PA signal by elevating thermal-to-acoustic conversion and its higher light-harvesting capability at redshifted region. The simultaneously strong PA signal and photothermal conversion efficiency of p-TTmB NPs enable precise PA imaging and effective photothermal therapy. This work highlights a simple and available design guideline of polymerization for amplifying the PA effect and optimizing existing materials.

Precision diagnosis and treatment have received increasing attention for modern medicine, and the optical imaging and therapeutic techniques (e.g., fluorescence imaging, photoacoustic imaging, time-resolved imaging, photodynamic therapy, and photothermal therapy) have greatly advanced this field. It is now becoming very clear that the photophysical properties play a central role in determining the biomedical function and efficiency of optical agents, thus it is extremely important to make as much absorbed energy as possible to dissipate in the desired pathway for maximized biomedical applications. During energy transition processes, intersystem crossing (ISC) is a crucial step that determines many fundamental photochemical and photophysical phenomena, such as photodynamic therapy, room-temperature phosphorescence, and thermally activated delayed fluorescence. Therefore, the manipulation of ISC process of organic molecules will greatly advance the development of high-performance phototheranostic agents. Thus far, although a few of review papers have summarized some achievements in optical imaging or therapeutic materials, few of them have concentrated on how to boost the photophysical properties and biomedical applications by tuning the ISC-related energy transfer process of organic molecules. In this review, we highlight the recent progresses of organic molecular probes for biomedical applications that are achieved via rational molecular design especially by manipulating the ISC-related photophysical process. The perspectives and challenges for future phototheranostic agent development via regulating ISC process are also discussed. This review aims to provide guidance for the design of organic optical agents with optimal energy transformation to maximize their performance in desired biomedical applications and in precision medicine.(c) 2022 Elsevier B.V. All rights reserved.

Lysosome-relevant cell death induced by lysosomal membrane permeabilization (LMP) has recently attracted increasing attention. However, nearly no studies show that currently available LMP inducers can evoke immunogenic cell death (ICD) or convert immunologically cold tumors to hot. Herein, we report a LMP inducer named TPE-Py-pYK(TPP)pY, which can respond to alkaline phosphatase (ALP), leading to formation of nanoassembies along with fluorescence and singlet oxygen turn-on. TPE-Py-pYK(TPP)pY tends to accumulate in ALP-overexpressed cancer cell lysosomes as well as induce LMP and rupture of lysosomal membranes to massively evoke ICD. Such LMP-induced ICD effectively converts immunologically cold tumors to hot as evidenced by abundant CD8+ and CD4+ T cells infiltration into the cold tumors. Exposure of ALP-catalyzed nanoassemblies in cancer cell lysosomes to light further intensifies the processes of LMP, ICD and cold-to-hot tumor conversion. This work thus builds a new bridge between lysosome-relevant cell death and cancer immunotherapy.

Far red/near infrared (FR/NIR) materials have attracted wide attention due to their great potential in various applications, particularly in bio-imaging.However, it is still a challenge to manufacture organic FR/NIR materials with quite high efficiency and long emission wavelength, owing to the dominance of non-radiative deactivation in the dissipation of absorbed light excitation energy when the electronic bandgap decreases.Herein, a series of donor-acceptor-donor (D-A-D) type compounds based on benzoselenidazole are developed through the regulation of molecular aggregation states by twisted conformation groups.In one hand, the compound TPE-DPA-Se (tetraphenylethylene-diphenylamine-benzoselenidazole) exhibits the best aggregation-induced emission (AIE) properties among these compounds.In another hand, the obtained TPE-DPA-Se showed over 150 nm Stokes shift, which can be used to avoid the interference between excitation and emission light, as well as the near-infrared emission spectrum away from the organism auto-fluorescence, which was beneficial for the bio-application.Next, density functional theory (DFT) calculation was carried out with Gaussian 09W program at B3LYP/6-31G** level to determine the highest occupied molecular orbital (HOMO), the lowest unoccupied molecular orbital (LUMO) distributions and the optimized structures of these compounds.Then TPE-DPA-Se was formulated into nanoparticles by nanoprecipitation method with an amphiphilic co-polymer 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (MPEG2000-DSPE) as the doping matrix.TPE-DPA-Se exhibited excellent aqueous diameter stability in nanoparticle-state, as well as impressive luminescence in aqueous system with higher efficiency of up to 16.48%, which are suitable for many fields.To evaluate the biocompatibility of the TPE-DPA-Se NPs, we further carried out 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay.The result indicated their negligible toxicity.Encouraged by the excellent performance of these luminogens in nanoparticle state, a successful 4T1 cells imaging was demonstrated.Next, to set up the tumor-bearing mouse model, the luciferase-expressed 4T1 cancer cells were injected into the healthy mice via intraperitoneal injection.After about 7 days, the abdominal metastatic tumors were formed with many nodules in the abdominal cavity.The fluorescent image can merged with bioluminescence image completely and image-guided tumor resection is verified in this work, particularly for the micro-sized tumor of intraoperative detection.

Pure organic persistent room temperature phosphorescence (RTP) materials have attracted wide attention owing to their great potential in various applications, particularly in bioimaging. However, it is still a challenge to manufacture organic RTP materials possessing quite high efficiency and long lifetime, owing to the high requirements for triplet excitons. In this study, a series of keto derivatives with efficient RTP in crystals are developed through the regulation of molecular aggregation states by simple alkyl groups, resulting in impressive luminescence performance with a longer lifetime and higher efficiency of up to 868 ms and 51.59%, respectively. All the alkyl-substituted derivatives exhibit bright RTP intensities after heavy grinding with a pestle, indicating their robust RTP features, which are suitable for many fields. Encouraged by the excellent RTP performance of these luminogens in the crystalline state, successful orthotopic lung tumor imaging with a high signal-to-background ratio (SBR) of 65 is demonstrated in this study to provide the promise of pure organic RTP materials for disease diagnosis, which hold the advantages of low autofluorescence interference and high signal-to-background ratio.

A facile synthesized AIE bioprobe was developed to detect peroxynitrite sensitively and specifically. This bioprobe can realize both in vitro ONOO− detection and in vivo visualization of inflammation.

For the fabrication of engineered tissue constructs by three-dimensional (3D) bioprinting technology, the cell viability will be significantly affected by the shear stress during extrusion process and the exposure of light for cross-linking. Microgels with independently controlled compartments were demonstrated to provide protection for cells encapsulation. Here, we proposed to prepare the core-shell structured microgels for cells encapsulation to prevent the cell damage from the shear stress in extrusion-based 3D printing processes. The core-shell structured microgels with core layer of type I collagen and shell layer of alginate were prepared using a onestep innovational microfluidics technology through a multichannel microfluidic device. In the microfluidic parameters, acetic acid concentration and flow rate ratios of water phase to oil phase were found to evidently affect the morphology of microgels and viability of encapsulated cells. Methacrylated silk fibroin (SilMA) and methacrylated gelatin (GelMA) were synthesized and blended with cell-laden microgels as bioinks to fabricate the 3Dprinted constructs. The increasing content of SilMA would decrease the pore size and increase the compression property of SilMA/GelMA hydrogels. Importantly, the microgels-containing SilMA/GelMA construct ensure the improved cell proliferation as compared to the SilMA/GelMA counterpart. Furthermore, the in vivo experiments demonstrated that Microgels-15%SilMA/GelMA construct exhibited good biocompatibility and better bone formation performance compared with 15%SilMA/GelMA construct. Therefore, the strategy of preparing cellladen microgels based on microfluidic technology to improve the survival rate of cells in the bioprinting process can be available and effective in development of tissue engineered constructs.

As a common method for postoperative adjuvant treatments of bladder tumor, chemotherapy encounters low tumor targeting, short tumor retention time and bad bioavailability in clinical applications, which result in unsatisfactory high chemotherapeutical doses, frequent administration and subsequent severe side effects. Herein, we innovatively introduced the enzyme-assisted assembly to construct a bladder tumor-specific transformable peptide prodrug (i.e. HCPT-FF-GFLG-EEYSA). The prodrug targeted bladder tumor through the specific binding capacity of YSA to EphA2 and underwent on-demand structural transformation intracellularly from micelles to fibrils catalyzed by cathepsin B (CtsB), of which EphA2 and CtsB are overexpressed on the outer membrane and in cytoplasm of bladder tumor cells, respectively. Comparing with hydroxycamptothecin (HCPT), the prodrug can prolong the drug retention time and release the active drug in a sustained manner, which in turn decrease the administration frequencies of chemotherapeutics and reduce the side toxicities, etc. This strategy provides an alternative for bladder tumor chemotherapeutics and shows great potential to inhibit the relapse of postoperative tumors.

At present, radio frequency heating is regarded as a novel approach for crude oil extraction from heavy oil reservoir. But in the absence of any external assistance, it is difficult to further improve its heating performance, especially for the reservoirs with poor thermal conductivity and low electrical conductivity. Therefore, to solve the problem, this paper proposes a hybrid technique that combines the radio frequency heating and injection of conductive medium into hydraulic fractures. A mathematical model is firstly established to describe the radio frequency heating process, and then the model is verified by the temperature data obtained from laboratory experiment. Finally, the effects of the conductive medium properties, fracture position and width on reservoir temperature distribution are analyzed. Simulation results indicate that the specific heat, thermal conductivity and density of conductive medium have no obvious effect on reservoir temperature distribution, but the electrical conductivity within the range of 10-30 S/m can apparently cause temperature variation. Additionally, the influence of fracture position on temperature distribution is tremendous, but the fracture width fails to impact the temperature distribution due to small width ranges. This investigation provides a better solution for radio frequency heating to obtain greater heat extraction performance.

Photoacoustic (PA) imaging emerges as a promising technique for biomedical applications. The development of new strategies to boost PA conversion without depressing other properties (e.g., fluorescence) is highly desirable for multifunctional imaging but difficult to realize. Here, we report a new phenomenon that active intramolecular motions could promote PA signal by specifically increasing thermal-to-acoustic conversion efficiency. The compound with intense intramolecular motion exhibits amplified PA signal by elevating thermal-to-acoustic conversion, and the fluorescence also increases due to aggregation-induced emission signature. The simultaneously high PA and fluorescence brightness of TPA-TQ3 NPs enable precise image-guided surgery. The preoperative fluorescence and PA imaging are capable of locating orthotopic breast tumor in a high-contrast manner, and the intraoperative fluorescence imaging delineates tiny residual tumors. This study highlights a new design guideline of intramolecular motion amplifying PA effect.