Arctic freshwater biodiversity is rapidly changing due to climate warming, resource extraction, infrastructure development, and landscape transformation. To improve understanding, predict future responses, and inform policy formulation, research needs must be clearly identified. Using a horizon scan survey, Arctic freshwater experts from government, international agencies, and Indigenous Peoples identified 77 biodiversity research questions with 17 highlighted as most important for near term assessment. These questions span nine thematic categories: biodiversity and taxonomic challenges, hydrological change, productivity and food webs, ecosystem connectivity, methods, monitoring and assessment, permafrost change, winter ecology, anthropogenic development, and Indigenous Knowledge. Climate change emerged as the major driver among all categories and research questions. A key priority identified was the urgent need for long-term, harmonized monitoring programs among Arctic countries. Multiple knowledge gaps detected suggest that circumpolar research collaborations are required to tackle these issues.

The present study seeks to examine the production and revenue of Norwegian micro, small and large hydropower plants (HPP) under variable climatic conditions and a stable energy market. Climatic conditions and demand for electricity are postulated as major factors that determine the operations of HPPs both on a daily and an annual basis. However, the importance of these effects is rarely evaluated. Here, we used hourly production during 2010-2018 from 1371 Norwegian micro, small and large HPPs to quantify the climatic and electricity demand effects on revenues in the three HPP categories that typically differ in their flexibility and ability to respond to high hourly prices both on a daily and an annual basis. For all three categories, the storage of water in a reservoir significantly increased daily and annual revenues. The effects of the number of starts differed among HPP categories, showing a negative relationship with revenue in micro HPPs and positive in large HPPs both at daily and annual levels, whereas for small HPPs the relationship switched from positive to negative on a daily and annual basis, respectively. For large HPPs, daily and annual revenues increased in dry years. For all three HPP categories the annual revenue was considerably higher during the positive phase of the North Atlantic Oscillation (NAO) and when the Northern Hemisphere Temperature (NHT) was warmer. The daily revenue for small HPPs decreased during the positive phase of the NAO. Our study demonstrates that hydropower production is vital in a variable energy market of renewable energies, and that climate and electricity demand have detectable effects on daily and annual revenues.

Arctic freshwater ecosystems are characterized by low temperatures, a short ice-free period, low productivity, and low biodiversity. There is only fragmented knowledge about High Arctic benthic communities and no information about their functional relationships. The environmental characteristics and food resources of five contrasting High Arctic stream reaches near Ny-Ålesund, Svalbard, were investigated. The streams represented a gradient in environmental harshness. In addition to hydrological, chemical, and physical characteristics, food resources, algal biomass, and benthic invertebrate assemblages were determined. Food web structure was investigated using stable isotope signatures of δ13C and δ15N. The streams varied significantly in their hydrology, water temperature, and water chemistry. There was a clear distinction of these characteristics between streams fed by glacial meltwater, snowmelt and groundwater, as well lake outflows. Zoobenthos abundance was significantly lower in the glacier-fed stream and was highest in the groundwater-fed stream, enriched with nutrients, flowing below a bird cliff. Chironomidae dominated in all streams, although the dominance and relative abundance of the subfamily Diamesinae varied between streams. Stable isotope ratios (δ13C, δ15N) of food sources (autochthonous – allochthonous matter) varied considerably within and between streams. Most of the benthic invertebrates were heterotrophic consumers, although the food source differed between streams, with some relying mainly on autochthonous resources (glacial stream), others on allochthonous resources (most other streams), and a combination of both food sources in the stream near the bird cliff. Based on the research data, which were at characterizing the actual ecosystem structure and function, the accelerated rate of climate change in the Arctic, coupled with glacial retreat and permafrost thaw, is expected to significantly alter catchment characteristics. These changes will, in turn, affect benthic communities, food resources, and food webs. The classification of stream types according to their water source and catchment properties offers a robust framework for future monitoring efforts.

We investigated assemblages and distribution of Ephemeroptera, Plecoptera and Trichoptera in a mountain stream catchment and their relationship to environmental variables. Sampling stations were located along an altitudinal gradient from subalpine birch forest to low alpine tundra in the & Oslash;vre Heimdalen Valley in the Norwegian Jotunheimen Mountains. The aquatic insects were sampled using a kick net in June, July and September. At the same time environmental variables (e.g., water temperature, substrate composition) were measured and water samples taken for analysis. The mayfly, Baetis rhodani (Pictet, 1843) (Ephemeroptera) dominated all reaches, except the outlet of & Oslash;vre Heimdalsvatn Lake, where the trichopteran filter feeder Polycentropus flavomaculatus (Pictet, 1834) was recorded as the most abundant species. Along with increase in altitude, there was a decrease in taxa richness. The main environmental variables explaining species distribution were discharge, substrate size and water temperature. Canonical Correspondence Analysis (CCA) clearly separated the outlet of the & Oslash;vre Heimdalsvatn Lake, and the reach downstream of the small Brurskardtjern Lake from the other stream sites situated upstream of the & Oslash;vre Heimdalsvatn Lake. This indicates the distinction between alpine streams and alpine lake outlets where filter feeders are numerous, feeding on the abundant seston of lacustrine origin.

Regulatory audits have been judged as a vital instrument to ensure compliance with regulatory obligations. Similarity in understanding of the achievement of regulatory outcomes in both authorities and companies is a prerequisite to secure conformity to regulations. A divergent approach to audits suggests restricted effects of regulatory audits. We analysed nonconformity, documented in 153 audits of 113 Norwegian hydropower and aquaculture companies. Twenty companies were audited 2-12 times during 2012-2020. There were no indications that an audit resulted in improved awareness in the company. A major part of nonconformity is connected to financial interest, likely of significance to the company itself. In addition to the environmental consequences, the failure to uncover, correct, and prevent nonconformity is alarming, seen both from a risk governance and a financial perspective. Our study of water resource management and regulation shows that merely performing audits have no significant effect on improvement. We claim that it is futile to implement audits if they are not put into some systematic constructed programme theory in every single company. When stating nonconformity in regulatory audits, the rational way of following up by the authorities should focus on how the company and site managers in practice work with improving safety and quality.

Arctic terrestrial invertebrate biodiversity is generally poorly known, but the archipelago of Svalbard has one of the most up-to-date inventories of its terrestrial and freshwater faunas of any Arctic region, offering a baseline for long term monitoring of invertebrate communities in space and time. Since the most recent review of the Svalbard invertebrate fauna was produced in 2014, knowledge of this fauna has developed and this inventory is here critically revised and updated. Our aims are (1) to critically review the inventory based on current taxonomic knowledge, (2) publish the complete species inventory, including cross-referencing to the relevant publications, in an open access data archive (GBIF), and (3) highlight the particular advantages that working in Svalbard may bring for scientists working on terrestrial, freshwater, and cryospheric environments. The inventory contains a total 1091 valid species names or interim names. A gap analysis in the Barcode of Life Data Systems reveals that 50% of the species currently documented from Svalbard lack COI (Cytochrome c oxidase subunit 1) barcode reference sequences in this database. Gaps in our knowledge of the Svalbard fauna and how these may be resolved are discussed.

Human activities present significant threats to tropical freshwater ecosystems, notably in many global biodiversity hotspots, threats that are further increased by inadequate taxonomic knowledge and the lack of appropriate biomonitoring tools. This study integrates globally validated biomonitoring approaches with DNA-based identification methods to create a macroinvertebrate-based tool for diagnosing ecosystem health and assessing the biodiversity of tropical river ecosystems in Myanmar (Indo-Burma bioregion). To evaluate river site degradation, comprehensive data on water and habitat quality, as well as land use information, were collected. Riverine macroinvertebrates were sampled by kick sampling, and subsequent DNA barcoding analysis was used to establish molecular taxonomic units (MTUs) for key bioindicator groups, including Ephemeroptera, Plecoptera, Trichoptera, Coleoptera, and Odonata (EPTCO) as species-level identification nomenclature was lacking. Tolerance scores for the local fauna were derived along an environmental degradation gradient to enable comparisons with widely adopted global assessment tools relying on macroinvertebrate metrics. In both study areas, the upper parts of the river networks were generally undisturbed by human activities while stressors associated with urban and agricultural land use were evident in the lower parts of the catchments. The highest precision for assessment of river health was found when establishing tolerance scores adjusted to local species composition in each study area separately. Although a family-level-based multimetric approach was significantly related to the main environmental degradation gradient, assessments utilizing cryptic species-level data (MTUs) emerged as the being most precise indicator in both areas. Our study highlights the synergistic benefits of merging traditional biomonitoring with DNA-based methods for species identification for biomonitoring in tropical river ecosystems. To halt biodiversity decline and curb the extent of the escalating nature crisis, such integrated approaches will be highly valuable in understudied and biodiversity-rich aquatic ecosystems.

Global change threatens invertebrate biodiversity and its central role in numerous ecosystem functions and services. Functional trait analyses have been advocated to uncover global mechanisms behind biodiversity responses to environmental change, but the application of this approach for invertebrates is underdeveloped relative to other organism groups. From an evaluation of 363 records comprising >1.23 million invertebrates collected from rivers across nine biogeographic regions on three continents, consistent responses of community trait composition and diversity to replicated gradients of reduced glacier cover are demonstrated. After accounting for a systematic regional effect of latitude, the processes shaping river invertebrate functional diversity are globally consistent. Analyses nested within individual regions identified an increase in functional diversity as glacier cover decreases. Community assembly models demonstrated that dispersal limitation was the dominant process underlying these patterns, although environmental filtering was also evident in highly glacierized basins. These findings indicate that predictable mechanisms govern river invertebrate community responses to decreasing glacier cover globally.

Introduction: Arctic freshwater ecosystems are undergoing rapid environmental transformation because of climate change, which is predicted to produce fundamental alterations in river community structure and function. Methods: We explored how climate change affects benthic invertebrate communities of Arctic streams by examining patterns of their biological traits along latitudinal and climatic gradients in eastern North America (Canada) and northwestern Europe (Sweden, Norway). Results: Despite differences in taxonomic composition between continents, we identified similarities in the functional trait niche (FTN) of predominant macroinvertebrate taxonomic groups. Trait composition differed by latitude in eastern Canada, with a predominance of cold-tolerant taxa, tubular body shape, and cased and attached habits at the highest latitudes. Differences in trait composition were evident among ecoregions in Europe, with trait dominance at the highest latitudes that was comparable to North America. There was a similar increase in the relative abundance of cold tolerance and tubular body shape and a decrease in obligate shredders and trait richness with decreasing temperatures across both continents. Discussion: These patterns are indicative of FTNs that include physiological traits and habits that are advantageous for the low temperatures, short ice-free period, and low riparian vegetation cover at the highest latitudes. We predict that climate change will lead to an increase in functional diversity at high latitudes, as organisms with trait modalities that are currently only found at lower latitudes move northward. However, this change in trait composition will be mediated by the effect of spatial connectivity on dispersal ability, with slower change occurring on Arctic islands. These findings can support modelling of future change in Arctic freshwater assemblages in response to ongoing climate change.

Rivers of the Boreal Uplands vary greatly in size and hydrological regime. The most typical feature of all rivers is their importance in valleys as landscape elements and as key economic centers. To reduce perturbations and maintain catchments for coming generations, 388 parts of or entire catchments are protected through a special Protection Plan for Watercourses, and 52 rivers (represent 75% of the Atlantic salmon biomass in the region) have been selected as National Salmon Watercourses to protect areas for the wild Atlantic salmon. At present, two major challenges exist. A major interest in building small hydropower schemes (installed effect <10 MW) has taken place in recent years. The second challenge is related to floods and the use of land near river corridors. Problems arise in areas where the river corridor is a major part of the area available for human activity.

1. Warming in the Arctic is predicted to change freshwater biodiversity through loss of unique taxa and northward range expansion of lower latitude taxa. Detecting such changes requires establishing circumpolar baselines for diversity, and under -standing the primary drivers of diversity. 2. We examined benthic macroinvertebrate diversity using a circumpolar dataset of > 1,500 Arctic lake and river sites. Rarefied α diversity within catchments was as -sessed along latitude and temperature gradients. Community composition was

Arctic freshwaters support biota adapted to the harsh conditions at these latitudes, but the climate is changing rapidly and so are the underlying environmental filters. Currently, we have limited understanding of broad-scale patterns of Arctic riverine biodiversity and the correlates of alpha- and beta-diversity. Using information from a database set up within the scope of the Arctic Council's Conservation of Arctic Flora and Fauna Circumpolar Biodiversity Monitoring Plan, we analysed patterns and correlates of alpha- and beta-diversity in benthic diatom and macroinvertebrate communities across northern Norway, Sweden, and Finland. We analysed variation in total beta-diversity and its replacement and richness difference components in relation to location of the river reach and its drainage basin (Baltic Sea in the south, the Barents Sea in the east and the north, and the Norwegian Sea in the west), in addition to climate and environmental variables. In both macroinvertebrates and diatoms, the replacement and richness difference components showed wide variation. For macroinvertebrates, the richness difference component was the more important, whereas for diatoms, the replacement component was the more important in contributing to variation in beta-diversity. There was no significant difference in beta-diversity between the three main drainage basins, but species composition differed among the drainage basins. Based on the richness difference component of beta-diversity, climate variables were most strongly associated with community variation in macroinvertebrates. In diatoms, both environmental and climate variables were strongly correlated with community compositional variation. In both groups, there were also significant differences in alpha-diversity among the three main drainage basins, and several taxa were significant indicators of one of these drainage basins. Alpha diversity was greater in areas with a continental climate, while the oceanic areas in the west harboured greatly reduced flora and fauna. The correlates of biodiversity were relatively similar in macroinvertebrates and diatoms. Climate variables, in particular temperature, were the most strongly associated with biodiversity patterns in the Arctic rivers of Fennoscandia. Sedimentary geology may be associated with increased productivity and, to a lesser extent, with sensitivity to acidification. There was considerable variation in community composition across Arctic Fennoscandia, indicating the necessity of protecting several stream reaches or even whole catchments within each region to conserve total riverine biodiversity. Furthermore, it is likely that the predicted changes in temperature in Arctic areas will influence riverine diversity patterns across Fennoscandia.

Water temperatures control life histories and diversity of aquatic species. Hydropower regulation, particularly in high head systems, alters natural water temperature regimes, which may have profound and long‐term impacts on aquatic environments. Temperatures in by‐pass sections and reaches affected by residual/environmental minimum flows fluctuate more than in natural flow regimes, driven more by influence of air temperatures. Reaches downstream of power plant outlets tend to become warmer in winter and colder in summer, driven by stratification behind the reservoir dam. In hydro‐peaked systems high‐low temperature effects may thus be aggravated. We review alterations of hydropower to natural thermal regimes, impacts on key organisms in terms of survival, development and behavioral thresholds, and potential mitigation measures, with focus on Atlantic salmon and brown trout in high northern latitude stream systems. Previous syntheses have focused mainly on flow changes and ecological impacts. Temperature effects may not always be correlated with flow changes, although there are some unique challenges with temperature changes in far northern latitudes, for example, related to the seasonal and colder climates. To help knowledge‐based management and identify potential knowledge gaps, we review how hydropower regulation may impact seasonal water temperatures, what impacts changes to stream system temperature regimes may have to key organisms, for example, Atlantic salmon and brown trout, and what adaptations and behavioral variations they may exhibit to respond to changed temperature regimes, and finally what good practices can be recommended for mitigating temperature impacts. This synthesis indicates that there are impacts to the fish and their supporting food webs, in particular related to growth and development, and the potential for negative impacts seems higher, and better studied, than positive impacts in northern river systems. Some of these impacts may be modified by directed hydropower regulation practices, but here effect studies and knowledge are limited.

Abstract. Long-term observations of ice phenology in lakes are ideal for studying climatic variation in time and space. We used a large set of observations from 1890 to 2020 of the timing of freeze-up and break-up, and the length of ice-free season, for 101 Norwegian lakes to elucidate variation in ice phenology across time and space. The dataset of Norwegian lakes is unusual, covering considerable variation in altitude (4–1401 m a.s.l.) and climate (from oceanic to continental) within a substantial latitudinal and longitudinal gradient (58.2–69.9° N; 4.9–30.2° E). The average date of ice break-up occurred later in spring with increasing altitude, latitude and longitude. The average date of freeze-up and the length of the ice-free period decreased significantly with altitude and longitude. No correlation with distance from the ocean was detected, although the geographical gradients were related to regional climate due to adiabatic processes (altitude), solar radian (latitude) and the degree of continentality (longitude). There was a significant lake area effect as small lakes froze-up earlier due to less volume. There was also a significant trend that lakes were completely frozen over later in the autumn in recent years. After accounting for the effect of long-term trends in the large-scale NAO index, a significant but weak trend over time for earlier ice break-up was detected. An analysis of different time periods revealed significant and accelerating trends for earlier break-up, later freeze-up and completely frozen lakes after 1991. Moreover, the trend for a longer ice-free period also accelerated during this period, although not significant. An understanding of the relationship between ice phenology and geographical parameters is a prerequisite for predicting the potential future consequences of climate change on ice phenology. Changes in ice phenology will have consequences for the behaviour and life cycle dynamics of the aquatic biota.

The aim of this study is to generate a first global overview of pressures and methods used to assess the environmental quality of rivers and streams using macroinvertebrates. In total, 314 peer-review studies were reviewed, published in the period 1997 – 2018, from developing economies in Africa, South-Central America, Mexico and Southern Asia. To establish a global perspective, the results from the literature review were compared to other compiled datasets, biomonitoring manuals, environmental surveys and literature reviews from Europe, North America and Australasia. The literature review from the developing economies showed that sampling was most usual during baseflow, using kick- or Surber sampling, with taxonomical identification levels mostly to genus or family. Assessments were most often done using metrics (singular and multimetrics; > 70% of the applications) and were based on community attributes related to richness and dominance (58% of studies), sensitivity (40%), diversity by heterogeneity (32%) and functional traits (25%). Within each category, the most used metrics were the richness and dominance of Ephemeroptera, Plecoptera and Trichoptera (EPT), Biological Monitoring Working Party scoring systems (BMWP/ASPT), Shannon-Wiener diversity and feeding traits. Overall, 92% of the reviewed studies reported that the use of macroinvertebrates, at least in some of their responses, was successful in detecting degradation of environmental quality in the investigated rivers. Given the many similarities in applied methods worldwide, at present, we consider that a global assessment of riverine environmental quality can be feasible by using family level identifications of macroinvertebrate samples. We propose a global common metric (multimetric), comprising three of the most common river assessment metrics from the reviewed literature, but also elsewhere, namely the BMWP/ASPT, Shannon-Wiener diversity and richness of EPT. Recent concerns regarding the global state of nature and consequences for freshwater communities, as reported by the intergovernmental science-policy platform on biodiversity and ecosystem services (IPBES), emphasize the urgent need for such a synthesis.

Anthropogenic inputs of nutrients and organic matter are common in tropical lowland rivers while little is known about the pollution-induced changes in oxygen availability and respiratory performance of ectotherms in these high temperature systems. We investigated the effects of agriculture and urban land-use on river water oxygen levels (diel measurements), decomposition rates (Wettex) and macroinvertebrate assemblages (field studies), as well as the oxy-regulatory capacity of eight riverine macroinvertebrate taxa (laboratory study) from a tropical lowland river network in Myanmar. The highest decomposition rates (0.1-5.5 mg Wettex degree day-1) and oxygen stress (≤91% saturation deficits) were found in reaches draining degraded catchments with elevated concentrations of nutrients. All individual macroinvertebrate taxa investigated were to some extent able to regulate their respiration when placed under oxygen stress in the laboratory (regulation value of 0.74-0.89). The oxy-regulation capacity of macroinvertebrate assemblages in the river network were, as predicted, inversely related to diel oxygen stress (maximum deficit; lm, R2 = 0.69), where taxonomic richness and pollution sensitivity (ASPT metric) also declined sharply (lm, R2 ≥ 0.79). Our study shows that eutrophication and organic pollution induce oxygen deficits in tropical rivers but stimulate decomposition rates, which may further deplete oxygen levels. Furthermore, macroinvertebrate oxy-regulatory capacity predicts assemblage composition along gradients in oxygen stress at the ecosystem level. Our findings suggest that tropical lowland river systems could be highly sensitive to pollution by nutrients and organic matter leading to substantial impacts on ectotherm community composition and ecosystem functioning.

The helminth fauna of brown trout (Salmo trutta) in the Norwegian subalpine lake, Øvre Heimdalsvatn was studied by examination of gills, eyes, body cavity, kidney, stomach, pyloric region and intestine in a total of 112 brown trout randomly sampled in June, July, and September 2011. Ten helminth species, Discocotyle sagittata, Phyllodistomum umblae, Crepidostomum farionis, C. metoecus, Diplostomum sp., Proteocephalus sp., Cyathocephalus truncatus, Dibothriocephalus ditremus, D. dendriticus, and Capillaria sp. were identified. These data were compared to data from the period 1969 to 1972, just after the first record of the European minnow (Phoxinus phoxinus) in 1969. All ten helminth species, except D. dendriticus, were also present in 1969–72. However, a few major changes in infection intensities have occurred. The cestode D. ditremus and the trematode Diplostomum sp., both with piscivorous birds as final hosts, had markedly higher relative densities (abundance) in brown trout in 2011 compared to 1969–72, while the two Crepidostomum species showed a substantial decline in relative densities. We suggest that these changes may be indirectly related to the establishment and subsequent population increase of European minnow in the lake. The abundance of minnows may have increased the food basis for the piscivorous birds, primarily mergansers and the black-throated diver that now regularly forage in the lake. In addition, there have been changes in the littoral invertebrate community, including species serving as intermediate hosts of some of the brown trout parasites.

Long-term observations of ice phenology in lakes are ideal for studying climatic variation in time and space. We used a large set of observations from 1890 to 2020 of the timing of freeze-up and break-up, and the length of ice-free season, for 101 Norwegian lakes to elucidate variation in ice phenology across time and space. The dataset of Norwegian lakes is unusual, covering considerable variation in elevation (4–1401 m a.s.l.) and climate (from oceanic to continental) within a substantial latitudinal and longitudinal gradient (58.2–69.9∘ N, 4.9–30.2∘ E). The average date of ice break-up occurred later in spring with increasing elevation, latitude and longitude. The average date of freeze-up and the length of the ice-free period decreased significantly with elevation and longitude. No correlation with distance from the ocean was detected, although the geographical gradients were related to regional climate due to adiabatic processes (elevation), radiation (latitude) and the degree of continentality (longitude). There was a significant lake surface area effect as small lakes froze up earlier due to less volume. There was also a significant trend that lakes were completely frozen over later in the autumn in recent years. After accounting for the effect of long-term trends in the large-scale North Atlantic Oscillation (NAO) index, a significant but weak trend over time for earlier ice break-up was detected. An analysis of different time periods revealed significant and accelerating trends for earlier break-up, later freeze-up and completely frozen lakes after 1991. Moreover, the trend for a longer ice-free period also accelerated during this period, although not significantly. An understanding of the relationship between ice phenology and geographical parameters is a prerequisite for predicting the potential future consequences of climate change on ice phenology. Changes in ice phenology will have consequences for the behaviour and life cycle dynamics of the aquatic biota.

Freshwater ecosystems in the Indo-Burma biodiversity hotspot face immediate threats through habitat loss and species extinction. Systems to monitor ecological status and trends in biodiversity are therefore crucially needed. Myanmar is part of Indo-Burma but with no past experience of biomonitoring in freshwaters. In this study, we aimed to assess the ecological and biodiversity status of a lowland river network in south-central Myanmar by identifying and quantifying pressures using macroinvertebrates as bioindicators. Novel data on water quality (nutrients, sediments and metals), hydromorphology (Morphological Quality Index; MQI), habitat quality (Litter-Siltation Index; LSI), land use, and macroinvertebrates were collected from 25 river sites. The dominant pressures on rivers were urban land use, inputs of untreated sewage, in-stream and riparian garbage littering, run-off from agricultural fields and plantations, as well as physical habitat degradation. Water chemistry data indicated inputs of sediments and nutrients to degraded streams, but no obvious metal pollution. The LSI and MQI indices indicated high perturbation in agricultural and urban areas, respectively. Ecological status was assessed using a first version of a modified Average Score per Taxon index (ASPT), while biodiversity was assessed by family richness within the orders Ephemeroptera, Plecoptera, Trichoptera, Coleoptera and Odonata (EPTCO), which was tested against the pressure gradient by principal component regressions. ASPT had high diagnostic capabilities (R2 = 0.68, p < 0.001) and showed that the index can be used to evaluate ecological water quality in this region. Biodiversity, expressed as family richness, also declined along the gradient (R2 = 0.59, p = 0.041), giving support to the fact that current land-use practices in this area are unsustainable.

Freshwater chemistry across the circumpolar region was characterised using a pan-Arctic data set from 1,032 lake and 482 river stations. Temporal trends were estimated for Early (1970-1985), Middle (1986-2000), and Late (2001-2015) periods. Spatial patterns were assessed using data collected since 2001. Alkalinity, pH, conductivity, sulfate, chloride, sodium, calcium, and magnesium (major ions) were generally higher in the northern-most Arctic regions than in the Near Arctic (southern-most) region. In particular, spatial patterns in pH, alkalinity, calcium, and magnesium appeared to reflect underlying geology, with more alkaline waters in the High Arctic and Sub Arctic, where sedimentary bedrock dominated. Carbon and nutrients displayed latitudinal trends, with lower levels of dissolved organic carbon (DOC), total nitrogen, and (to a lesser extent) total phosphorus (TP) in the High and Low Arctic than at lower latitudes. Significantly higher nutrient levels were observed in systems impacted by permafrost thaw slumps. Bulk temporal trends indicated that TP was higher during the Late period in the High Arctic, whereas it was lower in the Near Arctic. In contrast, DOC and total nitrogen were both lower during the Late period in the High Arctic sites. Major ion concentrations were higher in the Near, Sub, and Low Arctic during the Late period, but the opposite bulk trend was found in the High Arctic. Significant pan-Arctic temporal trends were detected for all variables, with the most prevalent being negative TP trends in the Near and Sub Arctic, and positive trends in the High and Low Arctic (mean trends ranged from +0.57%/year in the High/Low Arctic to -2.2%/year in the Near Arctic), indicating widespread nutrient enrichment at higher latitudes and oligotrophication at lower latitudes. The divergent P trends across regions may be explained by changes in deposition and climate, causing decreased catchment transport of P in the south (e.g. increased soil binding and trapping in terrestrial vegetation) and increased P availability in the north (deepening of the active layer of the permafrost and soil/sediment sloughing). Other changes in concentrations of major ions and DOC were consistent with projected effects of ongoing climate change. Given the ongoing warming across the Arctic, these region-specific changes are likely to have even greater effects on Arctic water quality, biota, ecosystem function and services, and human well-being in the future.