Annals of the New York Academy of SciencesVolume 459, Issue 1 p. 239-244 Neon-20 Ion- and X-Ray-Induced Mammary Carcinogenesis in Female Rats CLAIRE J. SHELLABARGER, CLAIRE J. SHELLABARGER Medical DepartmentSearch for more papers by this authorJOHN W. BAUM, JOHN W. BAUM Safety and Environmental Protection Division Brookhaven National Laboratoryc Upton, New York 11973Search for more papers by this authorSEYMOUR HOLTZMAN, SEYMOUR HOLTZMAN Medical DepartmentSearch for more papers by this authorJ. PATRICK STONE, J. PATRICK STONE Medical DepartmentSearch for more papers by this author CLAIRE J. SHELLABARGER, CLAIRE J. SHELLABARGER Medical DepartmentSearch for more papers by this authorJOHN W. BAUM, JOHN W. BAUM Safety and Environmental Protection Division Brookhaven National Laboratoryc Upton, New York 11973Search for more papers by this authorSEYMOUR HOLTZMAN, SEYMOUR HOLTZMAN Medical DepartmentSearch for more papers by this authorJ. PATRICK STONE, J. PATRICK STONE Medical DepartmentSearch for more papers by this author First published: December 1985 https://doi.org/10.1111/j.1749-6632.1985.tb20831.xCitations: 12 Brookhaven National Laboratory is operated by Associated Universities, Inc., under contract to the U.S. Department of Energy (Contract DE-AC02-CH00016). AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat References 1 Alpen, E. 1977. National Biomedical Heavy-Ion Research Program at the BEVALAC. In Biological and Medical Research with Accelerated Heavy Ions at the BEVALAC 1974-1977. Pp. 13–25. Lawrence Berkeley Laboratory Report LBL-5610. University of California. Berkeley , Calif. (Available from National Technical Information Service, Springfield, Va. 22161.). 2 Tobias, C. A., E. V. Benton & M. P. Capp 1977. Heavy-ion radiography. In Biological and Medical Research with Accelerated Heavy Ions at the BEVALAC 1974-1977. Pp. 164–186. Lawrence Berkeley Laboratory Report LBL-5610. University of California. Berkeley , Calif. (Available from National Technical Information Service, Springfield, Va. 22161.). 3 McGregor, D. H., C. E. Land, K. Choi, S. Tokuoka, P. I. Liu, T. Wakabayashi & G. W. Beebe 1977. Breast cancer incidence among atomic bomb survivors, Hiroshima and Nagasaki. J. Natl. Cancer Inst. 59: 799–811. 4 Shore, R. E., L. H. Hemplmann, E. Kowaluk, P. S. Mansur, B. S. Pasternac, R. E. Albert & G. E. Haugie 1977. Breast neoplasms in women treated with X-rays for acute postpartum mastitis. J. Natl. Cancer Inst. 59: 813–822. 5 Boice, J. D., Jr & R. R. Monson 1977. Breast cancer in women after repeated fluorescopic examinations of the chest. J. Natl. Cancer Inst. 59: 823–832. 6 Bond, V. P., C. J. Shellabarger, E. P. Cronkite & T. Fliedner 1960. Studies on radiation-induced mammary gland neoplasia in the rat. V. Induction by localized radiation. Radiat. Res. 13: 318–328. 7 Mackenzie, I. 1965. Breast cancer following multiple fluoroscopies. Br. J. Cancer 19 1–8. 8 Lyman, J. T. & J. Howard 1977. Biomedical research facilities and dosimetry. In Biological and Medical Research with Accelerated Heavy Ions at the BEVALAC 1974-1977. Pp. 26–35. Lawrence Berkeley Laboratory Report LBL-5610. Univesity of California. Berkeley , Calif. (Available from National Technical Information Service, Springfield, Va. (22161.). 9 Young, S. & R. C. Hallowes 1973. Tumors of the mammary gland. 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Induction of mammary neoplasms in the Sprague-Dawley rat by 430 keV neutrons and X-rays. J. Natl. Cancer Inst. 64: 821–833. 14 Vogel, H. H., Jr 1969. Mammary gland neutron irradiation. Nature 222: 1279–1289. 15 Clifton, K. H., E. B. Douple & B. N. Sridharan 1976. Effects of grafts of single anterior pituitary glands on the incidence and type of mammary neoplasms in neutron-or gamma-irradiated Fischer female rats. Cancer Res. 36: 3732–3735. 16 Montour, J. L., R. C. Hard & R. E. Flora 1977. Mammary neoplasia in the rat following high-energy neutron irradiation. Cancer Res. 37: 2619–2623. 17 Broerse, J. J., S. Knaan, & D. W. Van Bekkum, A. L. Nooteboom & C. F. Hollander 1977. Incidence of mammary tumors in rats of different strains after fast neutron irradiation. Int. 3. Radiat. Biol. 31: 378–379. 18 Yokoro, K., M. Nakano, A. Ito, K. Nago, Y. Kadama & K. Hamada 1977. Role of prolactin in rat mammary carcinogenesis: detection of carcinogenicity of low-dose carcinogens and persisting dormant cancer cells. J. Natl. Cancer Inst. 58: 1777–1783. 19 Shellabarger, C. J., E. P. Cronkite, V. P. Bond & S. W. Lippincott 1957. The occurrence of mammary tumors in the rat after sublethal whole-body irradiation. Radiat. Res. 6: 501–512. Citing Literature Volume459, Issue1Hematopoietic Cellular Proliferation: An International Conference in Honor of Eugene P. CronkiteDecember 1985Pages 239-244 ReferencesRelatedInformation

Tyrosinase was first detected in melanoblasts by the DOPA-oxidase reaction in the presence of catalase in explants of goldfish integument after 12 hr culture with either ACTH (1 IU/ml) or DB-cAMP (0.1m M). Melanin did not appear in the new melanocytes until 24 hr. The data indicate that the release of cAMP within the melanoblast in response to ACTH treatment is rapid and the tyrosinase in the melanoblast is released from inhibition and/or activated at least 12 hr prior to melanization of premelanosomes.

1.1. Comparison of methods for selectively removing epidermis from goldfish integument demonstrated that the use of EDTA provided efficient epidermis removal, excellent melanophore viability and good dermal cell preservation.2.2. Photometric measurement of melanophore response to epinephrine and to goldfish melanophore Ringer solution permitted quantitation of the degree of melanosome dispersion.3.3. Removal of the epidermis produced a tenfold increase in melanophore sensitivity to epinephrine and decreased post-treatment recovery rate. Thus, the epidermis is a diffusion barrier of considerable magnitude.4.4. Lipophores (xanthophores) and iridophores were unresponsive to epinephrine treatment (10 μg/ml) indicating that epinephrine is not involved in the normal hormonal control of these pigment cells.