Glycine, GABA, aspartate and glutamate are putative neurotransmitters in the mammalian spinal cord. The distribution of these amino acids was determined in the rat spinal cord. Transversely, the highest levels of glycine, aspartate and glutamate were in the intermediate and ventral gray; whereas, the highest levels of GABA were in the dorsal gray. Longitudinally, the highest levels of GABA and aspartate were in the thoracic and lumbar segments; whereas, the highest levels of glycine and glutamate were in the cervical and thoracic segments, respectively. In general, the distribution of these amino acids is similar to that reported for the cat, dog and human.

Abstract: The high‐affinity uptake of [3H]serotonin, [3H]glutamate, and [3H]‐γ‐aminobutyric acid (3H]GABA) and the Na+‐independent binding of [3H]glutamate and [3H]GABA were studied using spinal cord preparations obtained from normal mongrel dogs and from dogs made paraplegic by midthoracic spinal cord crush. Lumbosa‐cral regions of the spinal cord were removed either before (1 week) or after (3 to 8 weeks) onset of spasticity. A myelin‐free synaptosomal fraction was obtained by cen‐trifugation and used for studying high‐affinity uptake and for preparing synaptic plasma membranes for Na+‐inde‐pendent binding experiments. For the paraplegic groups, the uptake of 30 nM [3H]serotonin was 66 and 18% of control values after 1 and 3 weeks, respectively. Eadie‐Hofstee analysis of [3H]serotonin uptake showed a 90% reduction in Vmax for the paraplegic group relative to control values, thereby indicating the expected loss of descending serotonergic pathways. The high‐affinity uptakes of 1 μM [3H]glutamate and [3H]GABA were the same in both the control and nonspastic paraplegic groups after 1 week. However, after 3 weeks, the uptakes of [3H]glutamate and [3H]GABA were 60‐70% higher for the spastic group than for the control animals. For both amino acids, Eadie‐Hofstee plots revealed no difference in Km and higher Vmax for the spastic group relative to control values. After 1 and 3 weeks, the Na+ ‐independent binding of 5 nM [3H]glutamate was 40‐85% higher and the binding of 10 nM [3H]GABA was 40‐60% lower for the paraplegic groups relative to the values for the control animals. Scatchard analysis revealed significant changes in Bmax values for both amino acids. Overall, the data indicate an increase in segmental amino acid excitatory influence which occurred when signs of spasticity were evident.

Glycine, GABA, aspartate and glutamate are putative neurotransmitters in the mammalian spinal cord. The distribution of these amino acids was determined in the rat spinal cord. Transversely, the highest levels of glycine, aspartate and glutamate were in the intermediate and ventral gray; whereas, the highest levels of GABA were in the dorsal gray. Longitudinally, the highest levels of GABA and aspartate were in the thoracic and lumbar segments; whereas, the highest levels of glycine and glutamate were in the cervical and thoracic segments, respectively. In general, the distribution of these amino acids is similar to that reported for the cat, dog and human.

Glycine, GABA, aspartate and glutamate are putative neurotransmitters in the mammalian spinal cord. The distribution of these amino acids was determined in the rat spinal cord. Transversely, the highest levels of glycine, aspartate and glutamate were in the intermediate and ventral gray; whereas, the highest levels of GABA were in the dorsal gray. Longitudinally, the highest levels of GABA and aspartate were in the thoracic and lumbar segments; whereas, the highest levels of glycine and glutamate were in the cervical and thoracic segments, respectively. In general, the distribution of these amino acids is similar to that reported for the cat, dog and human.