Objective To study the anti-cancer effects of sanguinarine using two human cervical cancer HeLa and Siha cell lines.Methods MTr and colony formation were used to observe cell growth.Cell scratch test was performed to evaluate cell migration and invasion.Results After treatment with 1.0μmol/L and 5.0μmol/L SAN,the viability of Siha cells was 72%and 10%(P＜0.01),respectively.HeLa cells were more sensitive than Siha cells.After treatment with 1.0 μmol/L SAN,colony formation in HeLa and Siha cells decreased from 33 to 14 and 16 (P＜0.05).Cell scratch test showed cut width was(0.5 1±0.04)mm and(0.64±0.02)mm in the control and(1.22±0.02)mm and (1.63±0.01)mm in the HeLa and Siha cells treated with 0.5 μmol/L SAN(P＜0.01 and P＜0.05).MTT,colony formation and cell scratch test showed time-or dose-dependent manner.Conclusion SAN could inhibit the proliferation,migration and invasion of HeLa and Siha cells,which provide a laboratory evidence for potential application of SAN in clinical therapy of cervical cancer.

目的:改进复方薄荷脑滴鼻液中樟脑的鉴别方法.方法:差示分光光度法.结果:用配制复方薄荷脑滴鼻液的同一批号液状石蜡作参比,在289 nm波长处有最大吸收.结论:差示分光光度法鉴别复方薄荷脑滴鼻液中的樟脑,结果准确,方法简单.