Lymphocyte subsets in peripheral blood and function of CD4+CD25+ regulatory T cells(Tregs) were assayed in patients during different periods of Graves' disease ( GD ).Breakdown of Tregs' function lead to the wild proliferation of CD4+T lymphocyte,and may play an important role in the pathogenesis of GD.It is difficult to fully restore the Tregs' function to normal after successful medication in Graves' disease,this phenomenon may lead to easy relapse of GD.

Objective:To explore whether 131I reverted the immune disorder of Graves′ disease(GD) via changing the proportion of CD4+CD25+ Foxp3+ regulatory T cells(Tregs),we evaluated the peripheral lymphocyte subsets,Tregs and the expression of Foxp3 mRNA from GD patients before 131I administration and in the first month after 131I administration.Methods:Thirty GD patients treated by 131I were included in this study.The proportion of lymphocyte subsets in the peripheral blood from GD before 131I administration and in the first month after 131I administration were detected by flow cytometric analysis.Real-time polymerase chain reaction was performed also to measure the expression of Foxp3mRNA.Results:Compared to GD patients before 131I administration,the proportion of CD3-CD19+ B and CD3+CD4+T lymphocytes was significantly lower(P0.05),and the proportion of CD3-CD16+CD56+NK lymphocytes was significantly higher in GD patients in the first month after 131I administration(P0.05),the proportion of CD3-CD8+ T lymphocytes,Tregs and the expression of Foxp3mRNA could not be changed remarkably.Conclusion:Iodine-131 might not alleviate impairment of immunological tolerance via increasing the proportion of Tregs,while it might control excessive immunological response via decreasing the proportion of CD3-CD19+ B and CD3+CD4+T lymphocytes and increasing the proportion of NK lymphocytes.

Objective To explore the significance and the clinical value of the expression of AKT protein,PTEN in bone tumor.Methods The expression of AKT,PTEN had be detected for the specimens of 48 osteosarcoma and 15 exostosis with immunohistochemistry SABC method.Results The positive rate of AKT was 87.5 %(42/48)in osteosarcoma group and 33.3%(5/15)in excostosis group.There was a prominent difference between them(P<0.05).The positive expression rate of PTEN was 41.7%(20/48)in osteosarcoma group and 86.7%(13/15)in exostosis group.There was a prominent between them(P<0.05).The expression of AKT was negatively correlated with the expression of PTEN in osteosarcoma group(r-0.453,P<0.05).Protein level of AKT was higher in osteosarcoma recidivism and pulmonary metastasis than those no recidivism and no pulmonary metastasis(P<0.05).However,PTEN protein level was contrary.Both of them were not related with age,sex and size of tumor(P>0.05).Conclusion The hyper expression of AKT may play an important role in the initiation and development of human osteosarcoma,whereas,PTEN inhibit this process.