Ketoreductasehas been used in a synthesis of the lipid‐lowering agent Rosuvastatin .By high‐throughput screening ,KRED‐4has beenpicked up to prepare Rosuvastatin ethyl ester .After a preliminary optimization ,the enzymatic reactiontemperature 25℃ ~35℃ ,pH6 .0 ,initial substrate concentration1g/L and enzyme concentration 2g/Lhave been determined .In optimal conditions ,the substrate can be com‐pletely transformed into the product of Rosuvastatin ethyl ester .The configuration of the product is entire‐ly correct ,and the de>99 .9% ,providinga new idea for the green technology of Rosuvastatin prepared .

From the cheap raw materials fluorobenzene and pentanedioic acid ,ezetimibe has been synthe-sized by six steps reaction such as Friedel-Crafts reaction ,acylation reaction ,reduction reaction ,coupling reaction and cyclization etc .The total yield of ezetimibe is 7 .5% .The key step has been performed by KRED238 ketoreductase from the compound 5 and obtained a S configuration intermediate compound 6 in a yield of 95% ,the dextrose equivalent value is 95 .68% .The reduction reaction has been carried out with a high stereoselectivity .The results demonstrate that the biocatalytic technology can be used for the synthe-sis of ezetimibe .It provides a new way for the development of green asymmetric synthesis of ezetimibe .

With threonine deaminase(L-TD) and leucine dehydrogenase(L-LeuDH) obtained by cloning and expression as biocatalyst,the natural amino acid was converted into unnatural amino acid using the "one-pot" process.Finally,the product was obtained by crystallization,with a total yield of 84%.The configuration of the product obtained was confirmed by LC-MS and 1HNMR,with ee99.9%.This process uses cheap raw materials and enzymes,and avoids the intercurrent extraction and separation,holding out a bright prospect for an efficient and low-cost 2-aminobutyric acid production process.

The important chiral intermediate(R)-2-hydroxy-4-phenyl butyric acid(R-HPBA)was prepared from prochiral compound,using lactate dehydrogenase(D-LDH)obtained by cloning and expression as the biocatalyst.The affecting factors such as pH,initial substrate concentration,enzyme loading,and NAD+ amount were investigated.Finally,the product was prepared from 100 mmol/L substrate in 4 h by a fed-batch process.The configuration of the target product obtained therein was confirmed,and the e.e 99.9%.