The polyacrylamide gel electrophoresis was used to analyze peroxidase isoenzyme,esterase isoenzyme,superoxide dismutase isoenzyme of five Leacaena leucacephal varieties and a variety of Acacia farnesiana(L.)Willd.According to isozyme bands characteristics,the genetic relationship and genetic diversity of six varieties were studied.The results showed that the number of zymogram bands,mobility,enzyme activity and distribution characteristics of the germplasm resources had difference in various degree.