In present study, association of five single nucleotide polymorphisms (SNPs) of database rs109421300, rs43691049, rs109727821, rs109047657 and rs135678421 was investigated with milk minerals including calcium (Ca), phosphorus (P), copper (Cu), zinc (Zn), manganese (Mn), iron (Fe), sodium (Na), potassium (K) and constituents (fat and protein percentage) after adjusting the records with various significant non-genetic factors in crossbred (Vrindavani) cattle. The effect of rs109421300 pertaining to Diacylglycerol Acyltransferase (DGAT) gene located on BTA 14, was highly significant on fat percentage and significant on test day milk yield and non-significant on Ca, Cu, Mn, Zn, Fe, Na, K, test day milk yield and protein percentage. The AA genotypes of rs109421300 locus had significantly lower (2.96±0.17%) fat percentage than AG (4.64±0.22%), GG (4.62±0.27%) genotypes but simultaneously cows with AA genotypes had significantly highest (11.59±0.51 kg) test day milk yield than other two genotypes. At locus rs109727821, the manganese concentration was significantly highest (1.64±0.16 mg/l) for AG followed by AA (0.98±0.15 mg/l) and GG (0.77±0.24 mg/l) genotypes. The other SNPs had no significant association with traits under investigation. The existing association suggested possibilities to select animals for specific minerals and constituent traits in crossbred population.

The present investigation was conducted to compare milk minerals and constituents in different bovine speciesand breeds. The genetic and non-genetic factors affecting milk fat, protein and milk mineral composition in crossbredcattle were investigated. The effect of breeds was highly significant on copper (Cu), zinc (Zn), manganese (Mn), sodium (Na), potassium (K), fat and protein, significant (p≤0.05) on calcium (Ca) and non-significant on phosphorus (P) and iron (Fe) content. The Ca, Cu, fat and protein were significantly higher in Murrah buffalo followed by Tharparkar cattle and crossbred cattle. However, Mn and Zn were higher in Tharparkar cattle followed by Murrah buffalo and crossbred cattle. Tharparkar milk was also rich in Na and K followed by crossbred cattle and Murrah buffalo. The effect of parity was highly significant only on Mn, whereas, effect of lactation stage (LS) was significant on Fe and Cu in milk of crossbred cattle. The effect of Test Day Milk Yield (TDMY) was significant on Fe and Cu. The product moment correlation was highest (0.414) between Zn and Fe and lowest (-0.347) between Cu and Zn. Among milk minerals, the estimates of heritability for Fe, Ca and Mn were moderate, where as it was low for otherminerals in crossbred cattle.