‘Essex’ (Reg. No. CV‐33, PI 657980; experimental designation LC01602307E) lentil (Lens culinaris Medik.) was released by the USDA‐ARS in August 2009. Essex, a small‐seeded lentil with yellow cotyledons and green seed coat, was released based on its yield performance relative to that of the cultivar Eston. Essex is an F12 line derived from an F5 selection from a ‘Richlea’//PI 297754 cross. Essex was tested in advanced yield trials for a total of 16 site‐years in the U.S. Pacific Northwest (Idaho and Washington). Over all environments, Essex averaged 1300 kg ha−1, which was 35 and 26% greater than that of Eston and Athena, respectively. Essex reaches maturity at approximately 99 d, 1 d later than Eston. The green‐plant height of Essex averaged 37.2 cm, as compared with 32.6 cm for Eston. The mature‐plant height of Essex averaged 33.2 cm, as compared with 31.6 cm for Eston. However, difference between Essex and Eston for maturity, green‐plant height, and mature‐plant height were not significant. The mean 100‐seed weight of Essex was 4.3 g, which is significantly greater than that of Eston (3.5 g 100 seed−1). Essex will be targeted for production in the Pacific Northwest of the United States and is expected to be exported primarily to Mexico.

Diversity of powdery mildew pathogens infecting pea (Pisum sativum) in the US Pacific Northwest was investigated using both molecular and morphological techniques. Phylogenetic analyses based on rDNA ITS sequences, in combination with assessment of morphological characters, defined two groups of powdery mildews infecting pea. Group I (five field samples and three glasshouse samples) had ITS sequences 99% similar to those of Erysiphe pisi in GenBank and exhibited simple, mycelioid type of chasmothecial appendages typical of E. pisi. Erysiphe pisi is normally considered as the powdery mildew pathogen of pea. Group II (four glasshouse samples and two field samples) had ITS sequences 99% similar to those of E. trifolii and produced chasmothecia with dichotomously branched appendages similar to those of E. trifolii. There are fourteen nucleotide differences in the ITS region between the two groups. The correlation of rDNA ITS sequences with teleomorphic features for each of the two groups confirms their identity. Repeated samplings and artificial inoculations indicate that both E. pisi and E. trifolii infect pea in the US Pacific Northwest. Erysiphe trifolii is not previously known as a pathogen of pea. The existence of two distinct powdery mildew species infecting pea in both glasshouse and field environments may interfere with the powdery mildew‐resistance breeding programmes, and possibly explains putative instances of breakdown of resistance in previously resistant pea breeding lines.

The pea weevil, Bruchus pisorum (L.), is one of the most intractable pest problems of cultivated pea, Pisum sativum L., in the world. This study investigated the transfer of pea weevil resistance from two accessions (PI 595946, PI 343955) of wild pea, Pisum fulvum Sibth. & Sm., to interspecific populations derived from crossing these accessions with a weevil-susceptible pea cultivar ('Alaska 81'). Partial life tables characterized weevil stage-specific mortality and survivorship on parents and interspecific progeny in two glasshouse trials. Larval mortality rates on pods (F-3 plants) of several F-2:3 families were between 36.0% and 52.9%. These means were statistically similar to mean mortality rates on pods of resistant parents (45.4% and 46.2%), but significantly greater than mean rates on the susceptible parent (1.2% and 10.6%). Pod surface characteristics contributed to high neonate larval mortality on pods of resistant parents and interspecific progeny. Seed resistance was not broadly transferred to interspecific progeny [revealed by high weevil survivorship in seeds (means mostly > 80%) and high seed damage ratings of 3-5 where ratings of 1-2 denote resistance (production of resistant seed averaged 4.2% to 22.8%)]. Estimates of total weevil mortality on pods and seeds of eight F-2:3 families were 50-70%. Thus, weevil resistance in the Pisum secondary gene pool can be transferred to interspecific progeny, thereby providing a potential avenue to develop weevil-resistant pea cultivars.

In Oct. 2007, powdery mildew was found in chickpea fields in an experimental farm near Pullman, Whitman County, Washington. Although disease signs were observed on all chickpea cultivars in the fields, high incidence was seen only on cvs. Dwelley and Spanish White. To our knowledge this is the first record of powdery mildew caused by Leveillula taurica on chickpea in WA. The pathogen has also been reported from chickpea in California and elsewhere, e.g., Ethiopia, India, Iran, Morocco, Pakistan, Sudan, Turkey, and the former USSR. Accepted for publication 4 May 2008. Published 2 July 2008.

Journal of Plant RegistrationsVolume 1, Issue 2 p. 118-119 Cultivar Registration of ‘Specter’ Winter Feed Pea K. E. McPhee, Corresponding Author K. E. McPhee [email protected] USDA-ARS, 303 Johnson Hall, Washington State Univ., Pullman, WA, 99164-6434 Contribution from USDA-ARS in cooperation with the College of Agriculture and Home Economics, Agricultural Research Center, Washington State Univ., Pullman, WA, 99164Corresponding author ([email protected]).Search for more papers by this authorF. J. Muehlbauer, F. J. Muehlbauer USDA-ARS, 303 Johnson Hall, Washington State Univ., Pullman, WA, 99164-6434 Contribution from USDA-ARS in cooperation with the College of Agriculture and Home Economics, Agricultural Research Center, Washington State Univ., Pullman, WA, 99164Search for more papers by this author K. E. McPhee, Corresponding Author K. E. McPhee [email protected] USDA-ARS, 303 Johnson Hall, Washington State Univ., Pullman, WA, 99164-6434 Contribution from USDA-ARS in cooperation with the College of Agriculture and Home Economics, Agricultural Research Center, Washington State Univ., Pullman, WA, 99164Corresponding author ([email protected]).Search for more papers by this authorF. J. Muehlbauer, F. J. Muehlbauer USDA-ARS, 303 Johnson Hall, Washington State Univ., Pullman, WA, 99164-6434 Contribution from USDA-ARS in cooperation with the College of Agriculture and Home Economics, Agricultural Research Center, Washington State Univ., Pullman, WA, 99164Search for more papers by this author First published: 01 September 2007 https://doi.org/10.3198/jpr2006.12.0826crcCitations: 13 All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL No abstract is available for this article. References Hagedorn, D.J., and Gritton, E.T. Registration of Wisconsin 7105 and 7106 pea germplasm. Crop Sci. 1971 11: 946. https://doi.org/10.2135/cropsci1971.0011183X001100060077x, http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=agrocropsoil&KeyUT=A1971L246700078&DestLinkType=FullRecord&DestApp=WOS_CPL&UsrCustomerID=523bbf5d2a868de7bbaeea0bc70ec0e4 Murray, G.A., and Slinkard, A.E. Fenn Austrian winter pea. Univ. Idaho. Agric. Exp. Sta. Curr. Info. Ser. 209. 1973 Provvidenti, R., Hampton, R.O., and Muehlbauer, F.J. G-1000, Marx's pea breeding line for multiviral resistance. Pisum Genet. 1991 23: 50– 52 Citing Literature Volume1, Issue2September 2007Pages 118-119 ReferencesRelatedInformation

Pea (Pisum sativum L.) has a genome of about 4 Gb that appears to share conserved synteny with model legumes having genomes of 0.2-0.4 Gb despite extensive intergenic expansion. Pea plant inventory (PI) accession 269818 has been used to introgress genetic diversity into the cultivated germplasm pool. The aim here was to develop pea bacterial artificial chromosome (BAC) libraries that would enable the isolation of genes involved in plant disease resistance or control of economically important traits. The BAC libraries encompassed about 3.2 haploid genome equivalents consisting of partially HindIII-digested DNA fragments with a mean size of 105 kb that were inserted in 1 of 2 vectors. The low-copy oriT-based T-DNA vector (pCLD04541) library contained 55 680 clones. The single-copy oriS-based vector (pIndigoBAC-5) library contained 65 280 clones. Colony hybridization of a universal chloroplast probe indicated that about 1% of clones in the libraries were of chloroplast origin. The presence of about 0.1% empty vectors was inferred by white/blue colony plate counts. The usefulness of the libraries was tested by 2 replicated methods. First, high-density filters were probed with low copy number sequences. Second, BAC plate-pool DNA was used successfully to PCR amplify 7 of 9 published pea resistance gene analogs (RGAs) and several other low copy number pea sequences. Individual BAC clones encoding specific sequences were identified. Therefore, the HindIII BAC libraries of pea, based on germplasm accession PI 269818, will be useful for the isolation of genes underlying disease resistance and other economically important traits.

Journal of Plant RegistrationsVolume 1, Issue 2 p. 117-118 Cultivar Registration of ‘Windham’ Winter Feed Pea K. E. McPhee, Corresponding Author K. E. McPhee [email protected] USDA-ARS, 303 Johnson Hall, WSU, Pullman, WA, 99164-6434Corresponding author ([email protected]).Search for more papers by this authorC. C. Chen, C. C. Chen Central Agriculture Research Center, Montana State University, Moccasin, MT, 59462 Contribution from USDA-ARS in cooperation with the College of Agriculture and Home Economics, Agricultural Research Center, Washington State Univ., Pullman, WA 99164Search for more papers by this authorD. M. Wichman, D. M. Wichman Central Agriculture Research Center, Montana State University, Moccasin, MT, 59462 Contribution from USDA-ARS in cooperation with the College of Agriculture and Home Economics, Agricultural Research Center, Washington State Univ., Pullman, WA 99164Search for more papers by this authorF. J. Muehlbauer, F. J. Muehlbauer USDA-ARS, 303 Johnson Hall, WSU, Pullman, WA, 99164-6434Search for more papers by this author K. E. McPhee, Corresponding Author K. E. McPhee [email protected] USDA-ARS, 303 Johnson Hall, WSU, Pullman, WA, 99164-6434Corresponding author ([email protected]).Search for more papers by this authorC. C. Chen, C. C. Chen Central Agriculture Research Center, Montana State University, Moccasin, MT, 59462 Contribution from USDA-ARS in cooperation with the College of Agriculture and Home Economics, Agricultural Research Center, Washington State Univ., Pullman, WA 99164Search for more papers by this authorD. M. Wichman, D. M. Wichman Central Agriculture Research Center, Montana State University, Moccasin, MT, 59462 Contribution from USDA-ARS in cooperation with the College of Agriculture and Home Economics, Agricultural Research Center, Washington State Univ., Pullman, WA 99164Search for more papers by this authorF. J. Muehlbauer, F. J. Muehlbauer USDA-ARS, 303 Johnson Hall, WSU, Pullman, WA, 99164-6434Search for more papers by this author First published: 01 September 2007 https://doi.org/10.3198/jpr2006.12.0828crcCitations: 12 All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat No abstract is available for this article. References McPhee, K.E., and Muehlbauer, F.J. Registration of ‘Specter’ winter feed pea. J. Plant Reg. 2007 1: 118–119. https://doi.org/10.3198/jpr2006.12.0826crc Provvidenti, R., Hampton, R.O., and Muehlbauer, F.J. G-1000, Marx's pea breeding line for multiviral resistance. Pisum Genet. 1991 23: 50–52 Citing Literature Volume1, Issue2September 2007Pages 117-118 ReferencesRelatedInformation

Crop ScienceVolume 47, Issue 1 p. 438-439 Registrations of Cultivar Registration of ‘Morton’ Winter-hardy Lentil F.J. Muehlbauer, Corresponding Author F.J. Muehlbauer muehlbau@wsu.edu USDA-ARS, Washington State University, Pullman, WA, 99164-6434Corresponding author (muehlbau@wsu.edu)Search for more papers by this authorK.E. McPhee, K.E. McPhee USDA-ARS, Washington State University, Pullman, WA, 99164-6434Search for more papers by this author F.J. Muehlbauer, Corresponding Author F.J. Muehlbauer muehlbau@wsu.edu USDA-ARS, Washington State University, Pullman, WA, 99164-6434Corresponding author (muehlbau@wsu.edu)Search for more papers by this authorK.E. McPhee, K.E. McPhee USDA-ARS, Washington State University, Pullman, WA, 99164-6434Search for more papers by this author First published: 01 January 2007 https://doi.org/10.2135/cropsci2005.12.0490Citations: 6 Contribution from USDA-ARS in cooperation with the College of Agriculture, Human and Natural Resource Sciences, Agricultural Research Center, Washington State Univ., Pullman, WA 99164. Registration by CSSA. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article.Citing Literature Volume47, Issue1January–February 2007Pages 438-439 RelatedInformation

Chickpea (Cicer arietinum L.) is cultivated as a rotational crop in the cereal-based production system in the U.S. Pacific Northwest (PNW) and its production is expanding to other northern tier states. During July 2005, symptoms of Sclerotinia stem rot were observed on chickpea cv. Dwelley and Dylan in fields near Spangle, WA and Carrington, ND, respectively, with disease incidence of approximately ≤1% in affected areas at both locations. Symptoms included stem whitening, wilting, and stem breakage. Occasionally, white fluffy mycelium was observed; however, production of sclerotia on infected plants was rarely observed. Sclerotinia sclerotiorum was isolated from diseased stems collected from both states. The isolates produced a ring of sclerotia near the edge of potato dextrose agar (PDA) plates in 7 days and produced neither conidia nor other fruiting bodies in culture after 30 days. PCR amplification of the rDNA internal transcribed spacer region from two representative isolates and subsequent digestion with restriction enzymes, Mbo I and Taq I, produced identical banding patterns to previously identified isolates of S. sclerotiorum from pea from the PNW (2). Chickpea cvs. Dwelley and Spanish White (eight plants of each) were inoculated by fastening mycelial agar plugs from an actively growing colony on PDA onto the stems with Parafilm. Symptoms of stem whitening were observed as early as 2 days after inoculation, and the lesions extended upward and downward from the inoculation site. Wilting and stem breakage were also observed. Control inoculations of four plants of each cultivar with PDA plugs without mycelium produced no visible symptoms. S. sclerotiorum was consistently reisolated from inoculated plants but not from control plants. Chickpea had been grown in the PNW for more than 20 years without any reported incidence of Sclerotinia stem rot although the disease has been reported from Arizona (3) and Asian countries (1). This is likely because of the upright growth habit of the chickpea plant coupled with relatively dry conditions late in the growing season. Previous chickpea cultivars were very susceptible to Ascochyta blight, an early-season disease of chickpea in the PNW that reduced chickpea stands and canopy coverage. Current cultivars possess much improved resistance to Ascochyta blight, allowing greater vegetative growth to occur and creating microenvironmental conditions conducive to Sclerotinia stem rot. In North Dakota, where humid conditions prevail late in the growing season, symptoms of Sclerotinia stem rot had been observed in previous years but had not been documented because of a recent history of chickpea cultivation there. To our knowledge, this is the first report of confirmed Sclerotinia stem rot of chickpea in North Dakota and Washington. References: (1) G. J. Boland and R. Hall. Can. J. Plant Pathol. 16:93, 1994. (2) I. Jimenez-Hidalgo et al. Phytopathology (Abstr.) 94(suppl.):S47, 2004. (3) M. E. Matheron and M. Porchas. Plant Dis. 84:1250, 2000.

A mini-dome bioassay was developed to study pathogenicity of Ascochyta rabiei and relative resistance of chickpea (Cicer arietanium). It was determined that the best condition for assaying pathogenicity of A. rabiei was to use 2 x 10(5) spores/ml as inoculum and to maintain a leaf wetness period of 24 h under mini-domes at a temperature between 16 and 22 degrees C. This mini-dome pathogenicity assay was used to determine relative resistance of six chickpea cultivars (cvs) to isolates of two pathotypes of A. rabiei. Grafting was employed to detect any translocated factors produced in the chickpea plant that mediate disease response, which could help elucidate possible resistance mechanisms to Ascochyta blight. The six chickpea cv. were grafted in all possible scion-rootstock combinations, and then inoculated with isolates of two pathotypes of A. rabiei using the mini-dome technique. Results showed that self-grafted-resistant plants remained resistant and self-grafted-susceptible plants stayed susceptible, indicating the grafting procedure did not alter host response to infection by A. rabiei. Susceptible scions always exhibited high and similar levels of disease severity regardless of rootstock genotypes, and resistant scions always showed low and similar levels of disease severity when they were grafted onto any of the six rootstock genotypes. Orthogonal contrasts showed that scion genotypes determined disease phenotype, and that rootstock genotypes had no contribution to disease phenotype of the scions. The pathogenicity assay did not detect any translocated disease-mediating agents responsible for susceptibility or resistance in chickpea. Disease phenotypes of Ascochyta blight of chickpea were conditioned locally by scion genotypes.

Crop ScienceVolume 44, Issue 4 p. 1488-1488 Registration of Cultivar Registration of ‘Pennell’ Lentil F.J. Muehlbauer, Corresponding Author F.J. Muehlbauer [email protected] USDA-ARS, Washington State University, Pullman, WA, 99164-6434Corresponding author ([email protected])Search for more papers by this authorK.E. McPhee, K.E. McPhee USDA-ARS, Washington State University, Pullman, WA, 99164-6434Search for more papers by this author F.J. Muehlbauer, Corresponding Author F.J. Muehlbauer [email protected] USDA-ARS, Washington State University, Pullman, WA, 99164-6434Corresponding author ([email protected])Search for more papers by this authorK.E. McPhee, K.E. McPhee USDA-ARS, Washington State University, Pullman, WA, 99164-6434Search for more papers by this author First published: 01 July 2004 https://doi.org/10.2135/cropsci2004.1488Citations: 6 Contribution from USDA-ARS in cooperation with the College of Agriculture and Home Economics, Agric. Res. Ctr., Washington State University, Pullman, WA 99164-6434. Registration by CSSA. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat References 1Muehlbauer, F.J., Registration of ‘Brewer’ and ‘Emerald’ lentil. Crop Sci. (1987) 27, 1088–1099 http://doi.org/10.2135/cropsci1987.0011183X002700050062x, http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=agrocropsoil&KeyUT=A1987J978600062&DestLinkType=FullRecord&DestApp=WOS_CPL&UsrCustomerID=523bbf5d2a868de7bbaeea0bc70ec0e4 2Muehlbauer, F.J., Registration of ‘Mason’ Lentil. Crop Sci. (2002) 42, 301–302 http://doi.org/10.2135/cropsci2002.0301, http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=agrocropsoil&KeyUT=000173296000047&DestLinkType=FullRecord&DestApp=WOS_CPL&UsrCustomerID=523bbf5d2a868de7bbaeea0bc70ec0e4 Citing Literature Volume44, Issue4July–August 2004Pages 1488-1488 ReferencesRelatedInformation

The effects of simulated climatic conditions on green color loss of peas (Pisum sativum) and related enzyme activities were investigated. Seeds of 2 green pea cultivars showing different resistance to green color bleaching were subjected to light only, soaking in water without light, and soaking in water with light. Green color loss and chlorophyll loss were highest in seeds soaked in water and exposed to light, less in seeds soaked in water under dark conditions, and least in seeds exposed to light only. increased chlorophyllase activity was associated with loss of green color and chlorophyll, whereas no significant relationship was found between chlorophyll loss and lipoxygenase or chlorophyll-degrading peroxidase activity. Susceptible and resistant cultivars had significant differences in green color, chlorophyll content, chlorophyll a/b ratio, and chlorophyll degradation kinetics constant when seeds were soaked in water and exposed to light. Enzyme activity was not significantly different between the cultivars. Soaking led to more green color loss, chlorophyll breakdown, and chlorophyllase activity than light exposure. Chlorophyllase may be the key enzyme responsible for green pea bleaching instead of the oxidative chlorophyll degradation pathway with lipoxygenase or chlorophyll-degrading peroxidase.