OBJECTIVE: To demonstrate that cellular preparations requiring color analysis of different domains stained by molecular cytogenetic methods (fluorescence in situ hybridization) can be processed by spectral analysis of fluorescent emissions by either factor analysis of medical image sequences (FAMIS) or a META confocal configuration to isolate fluorescent probes. STUDY DESIGN: Three-dimensional sequences of images obtained by spectral analysis in a META confocal microscope (Carl Zeiss SAS, Jena, Germany) were analyzed by META processing and the FAMIS algorithm, which provides factor curves. META and factor images were then the result of image-processing methods that cover emission spectra. RESULTS: Factor curves and factor or META images can help to analyze targets inside nuclei. CONCLUSION: It is possible to process preparations containing numerous spots on different colors to differentiate stained targets and to improve visualization and detection.

Fadi W. Abdul-Karim Pedro P. de Agustín Måns Åkerman M. Loredana Gadda Alasio Patricia Alonso de Ruiz Carmen Alvarez-Santin Ana-Cristina Anton Karen M. Atkison Silvana Audy-Jurkovic R. Marshall Austin Jan P. A. Baak Ulrik Baandrup Gunter F. Bahr Peter H. Bartels Sandra H. Bigner John W. Bishop Miklós Bodó Thomas A. Bonfiglio Mathilde E. Boon Gerhard Breitenecker Lukas Bubendorf Ursula Buerzele-Fricke Jorge Campos R. de C. Carol A. Carriere Tien-Chun Chang Patricia Chapman Annie N. Y. Cheung William N. Christopherson Douglas P. Clark Edith Claros Mercado Luiz Martins Collaco Brian T. Collins William N. Crabtree Peter Dalquen Dilip K. Das Diane D. Davey Denise DeFrias Margaret Douglas Manfred Droese Antonio Ducrot-Schinini Hormoz Ehya Yener S. Erozan Clarice Amaral Ferreira Norman W. Fitzgerald Brendan T. Fitzpatrick Alessandra M. Forni Hugo Galera-Davidson Kim R. Geisinger Claude Gompel Ricardo González-Cámpora Zenon Gonzalez-Romero Roberta M. Goodell Philippe de Graeve Shirley E. Greening Heinz K. Grohs Prabodh K. Gupta Steven I. Hajdu Xhevdet Harasani Ernst Held Michael R. Henry Manuel Hilgarth Anders Hjerpe J. Heinrich Holzner O. A. N. Husain Martha L. Hutchinson Hideo Ikeda Lucrecia Illescas Stanley L. Inhorn Matías Jiménez-Ayala William W. Johnston David B. Kaminsky Kusum Kapila Harubumi Kato Ruth L. Katz Tadao K. Kobayashi Jitka Kobilková Leopold G. Koss Helen A. Koutselini Rainer Kraft Paul A. Krieger Perikala V. Kumar Sow-Hsong Kuo Reviewers and Consultants