Heliamphora pulchella (Sarraceniaceae) is a species complex known and grown in cultivation for its trichome variation. SEM was used to investigate the micromorphology of 13 accessions of H. pulchella from specimens representing populations on six different tepuis. A total of 91 micrographs including various features related to trichome size and density as well as stomata and extra-floral nectaries (EFNs) were characterized both quantitatively and qualitatively. Location-based gradients in size and densities of trichomes, EFNs, and stomata exist across the tepuis examined and suggest further taxonomic investigation.

Background and aims – Vanilla subgenus Vanilla was established by Soto-Arenas and Cribb (2010) to include Neotropical species characterized by membranaceous leaves, a labellum without a penicillate callus, and a column attached solely at the base of the lip. Vanilla mexicana (= Epidendrum vanilla) was the first species attributed to this subgenus, and its nomenclature traces back to the Vanilla’s taxonomic origins, serving as the type species for the entire genus. Despite this, several biological aspects of membranaceous vanilla species remain poorly studied, including their taxonomy and nomenclature, which have not been thoroughly addressed. The lack of clarity regarding the circumscription of these fairly rare taxa has led to taxonomic confusion and neglect by botanists. This study aims to conduct a typological assessment of all names associated with Vanilla subgen. Vanilla. Material and methods – A comprehensive list of names attributable to Vanilla subgen. Vanilla was compiled from different sources. Protologues were studied for every name and type specimens were examined through direct visits or virtual herbaria. The International Code of Nomenclature of algae, fungi, and plants was followed for the type designation and nomenclatural notes. Key results – A comprehensive nomenclatural revision was carried out for 29 names. A total of eight lectotypes and one epitype are designated, with nomenclatural notes provided for most names within the subgenus.

The diversity of Vanilla Mill. in Andean South American countries remains significantly understudied, highlighting the urgent need for a robust taxonomic framework as a foundation for future biogeographic, monographic, phylogenetic, and ecological research. Drawing on extensive herbarium studies and fieldwork conducted by the authors over the past decade, we present a curated checklist of this economically important genus in South America, focused on the Andean countries of Bolivia, Colombia, Ecuador, and Peru. Our review records 31 Vanilla species across the four countries, with Colombia emerging as the most species rich, followed by Peru, Ecuador, and Bolivia. Among this diversity, we identified 25 species of potential interest as crop wild relatives of the globally cultivated V. planifolia Andrews. Moreover, we report several species not previously documented in our study area, including a new species from Ecuador, which we describe here as V. sekut Dami & aacute;n, Garz & oacute;n & Bentley. As part of our extensive herbarium and literature revision, we also designate six lectotypes, one neotype, and four epitypes. This checklist provides a critical baseline for future monographic and evolutionary studies on Vanilla in South America, offering valuable insights into its biodiversity and potential for agricultural and ecological applications.

Phylogenetic research in Tulipa (Liliaceae), a genus of significant economic and horticultural value, has relied on limited nuclear (mostly nuclear ribosomal internal transcribed spacer, nrITS) and plastid DNA sequences, resulting in low-resolution phylogenetic trees and uncertain intrageneric classifications. The genus, noted for its large genome, presents discordant relationships among Amana, Erythronium, and Tulipa, likely due to incomplete lineage sorting (ILS) and/or reticulate evolution. Thus, phylogenomic approaches are needed to clarify these relationships and the conflicting signals within the tribe Tulipeae. We newly sequenced 50 transcriptomes of 46 species of tribe Tulipeae (including multiple accessions of all four genera) and one outgroup species of the sister tribe Lilieae (Notholirion campanulatum), and downloaded 15 previously published transcriptomes of tribe Tulipeae to supplement the sampling. One plastid dataset (74 plastid protein-coding genes, PCGs) and one nuclear dataset (2594 nuclear orthologous genes, OGs) were constructed, with the latter used for species tree inference based on maximum likelihood (ML) and multi-species coalescent (MSC) methods. To investigate causes of gene tree discordance, “site con/discordance factors” (sCF and sDF1/sDF2) were calculated first, after which phylogenetic nodes displaying high or imbalanced sDF1/2 were selected for phylogenetic network analyses and polytomy tests to determine whether ILS or reticulate evolution best explain incongruence. Key relationships not resolved by this technique, especially those among Amana, Erythronium, and Tulipa, were further investigated by applying D-statistics and QuIBL. We failed to reconstruct a reliable and unambiguous evolutionary history among Amana, Erythronium, and Tulipa due to especially pervasive ILS and reticulate evolution, likely caused either by obscured minority phylogenetic signal or differing signals among genomic compartments. However, within Tulipa we confirmed the monophyly of most subgenera, with the exception of two species in the small subgenus Orithyia, of which Tulipa heterophylla was recovered as sister to the remainder of the genus, whereas T. sinkiangensis clustered within subgenus Tulipa. In contrast, most traditional sections of Tulipa were found to be non-monophyletic.

AimLycium L. (Solanaceae), which is known for producing goji berries, is an important plant with both medicinal and edible uses. This genus is globally distributed in temperate and subtropical regions. However, a comprehensive phylogeny and evolutionary history of this plant group is lacking so far. This study was executed to produce novel insights into the phylogenetic relationships and evolutionary history of this small but economically important genus.LocationNorth America, South America, Hawaii, Africa and Eurasia.TaxonLycium L. (Solanaceae).MethodsWe established a phylogenetic framework for Lycium based on complete plastome sequences and data from 80 protein-coding genes across 43 Lycium species using maximum likelihood and Bayesian inference methods. Furthermore, 14 species from the Solanaceae family were used as outgroups. Additionally, two Solanoideae fossils and one secondary calibration point were used to estimate divergence times and reveal the biographical history of these plants through ancestral area reconstruction.ResultsOur analysis revealed that six North American Lycium species were strongly supported as monophyletic with high support and were sister clades to the remainder of the genus. The remaining species from North America, South America and the Hawaiian Islands shared a common ancestor, whereas all species from Africa, Saharo-Arabia and Eurasia formed a distinct clade. Our results indicated that Lycium originated in North America during the Late Oligocene and then dispersed to Hawaii and South America, from there to Africa, and then further to Saharo-Arabia, with a more recent dispersal to Eurasia.Main ConclusionsOur plastid genome data confirmed that Lycium originated in North America and identified long-distance dispersal as the key to its global distribution. Genomic insights facilitate species identification and contribute to conservation efforts.

For few taxonomic groups do conservation efforts have such a disproportionate impact on biodiversity and human well-being as they do with bats. Bats face significant conservation challenges that affect their long-term viability, inhibit their ecosystem functions and services, and increase zoonotic spillover risks. Protecting bat populations and their habitats ultimately reduces these conservation threats, helps prevent pandemics, and supports essential ecosystem services. MENTOR-Bat is a fellowship program focused on strengthening technical research, and leadership capacity in the Global South to promote healthy environments where bats and humans can coexist with reduced risks of pathogen transmission. Co-designed by the United States Fish and Wildlife Service (USFWS) and Bat Conservation International (BCI), MENTOR-Bat mirrors the One Health framework by featuring a transdisciplinary team of three mentors and nine fellows from Cameroon, Colombia, and Indonesia. Fellows and mentors receive academic and field-based training on bat ecology and conservation, One Health, human dimensions of conservation, behavior change, strategic communications, international policy, adaptive management, project planning, conservation leadership, and public health. Fellows will then design and implement team pilot projects to advance One Health and bat conservation in their respective countries. Program evaluation of MENTOR-Bat is based on Kirkpatrick’s Hierarchy and focuses on measuring the development of established One Health core competences. By incorporating One Health and conservation within its activities, MENTOR-Bat can become a valuable programmatic template for transdisciplinary programming advancing evidence-based strategies for improving the well-being of bats, humans, and the environment.

Until now the genus Amana (Liliaceae), known as 'East Asian tulips', has contained just seven species. In this study, a phylogenomic and integrative taxonomic approach was used to reveal two new species, Amana nanyueensis from Central China and A. tianmuensis from East China. A. nanyueensis resembles Amana edulis in possessing a densely villous-woolly bulb tunic and two opposite bracts, but differs in its leaves and anthers. Amana tianmuensis resembles Amana erythronioides in possessing three verticillate bracts and yellow anthers, but differs in aspects of its leaves and bulbs. These four species are clearly separated from each other in principal components analysis based on morphology. Phylogenomic analyses based on plastid CDS further support the species delimitation of A. nanyueensis and A. tianmuensis and suggests they are closely related to A. edulis. Cytological analysis shows that A. nanyueensis and A. tianmuensis are both diploid (2n = 2x = 24), different from A. edulis, which is either diploid (northern populations) or tetraploid (southern populations, 2n = 4x = 48). The pollen morphology of A. nanyueensis is similar to other Amana species (single-groove germination aperture), but A. tianmuensis is quite different because of the presence of a sulcus membrane, which creates the illusion of double grooves. Ecological niche modelling also revealed a niche differentiation between A. edulis, A. nanyueensis and A. tianmuensis.

Orbiviruses are arthropod borne viruses of vertebrates, with some of them being important pathogens of veterinary, conservation and economic importance, while others are occasionally associated with human disease. Some apparently bat specific orbiviruses have been detected, but little is known about their distribution and diversity. We thus sampled and screened 52 bats living in the Congo Basin, and detected RNA indicative of a novel orbivirus in a single banana serotine (Afronycteris nanus) by PCR. The detected RNA clusters with epizootic haemorrhagic disease virus, bluetongue virus, and others. The findings highlight the need for more studies into arbovirus presence and diversity in bat species.

With 1.4 million specimens the Wisconsin State Herbarium (WIS) is one of the largest in the Americas and Wisconsin offers botanists a unique opportunity to study species representing a confluence of global biomes. The state harbors >2640 species of vascular plants which have been sequenced for the two-gene plant DNA barcode to reconstruct a community phylogeny. At the same time >300’000 georeferenced specimens were used with bioclimatic and soil data to produce species distribution models for the flora, then subsequently aggregated to determine current and future patterns of species richness and phylogenetic diversity. Among the many surprising results uncovered are predictions that whereas species richness will increase as c. 850 taxa move into the state, c. 242 species will become extirpated by 2070. These most vulnerable species will not be affected at random. Furthermore, models suggest that Wisconsin’s projected climate will be unsuitable for most species to be able to retain their present distributions; only 65 % will be able to retain more than half of their current distributions. However, the state’s well known unglaciated Driftless Area may be able to serve as an Anthropocene refugium better than anywhere else in the region and should be targeted for increased land conservation.

IntroductionPhylogenomics have been widely used to resolve ambiguous and controversial evolutionary relationships among plant species and genera, and the identification of unique indels in plastomes may even help to understand the evolution of some plant families.MenispermumL. (Menispermaceae) consists of three species,M. dauricumDC.,M. canadenseL., andM. mexicanumRose, which are disjuncly distributed among East Asia, Eastern North America and Mexico. Taxonomists continue to debate whetherM. mexicanumis a distinct species, a variety ofM. dauricum, or simply a synonym ofM. canadense. To date, no molecular systematics studies have included this doubtful species in phylogenetic analyses.MethodsIn this study, we examined phylogenomics and phylogeography ofMenispermumacross its entire range using 29 whole plastomes of Menispermaceae and 18 ITS1&ITS2 sequences of Menispermeae. We reconstructed interspecific relationships ofMenispermumand explored plastome evolution in Menispermaceae, revealing several genomic hotspot regions for the family.Results and discussionPhylogenetic and network analyses based on whole plastome and ITS1&ITS2 sequences show thatMenispermumclusters into two clades with high support values, Clade A (M. dauricum) and Clade B (M. canadense+M. mexicanum). However,M. mexicanumis nested withinM. canadenseand, as a result, we support thatM. mexicanumis a synonym ofM. canadense. We also identified important molecular variations in the plastomes of Menispermaceae. Several indels and consequently premature terminations of genes occur in Menispermaceae. A total of 54 regions were identified as the most highly variable plastome regions, with nucleotide diversity (Pi) values > 0.05, including two coding genes (matK,ycf1), four introns (trnK intron,rpl16intron,rps16 intron,ndhA intron), and 48 intergenic spacer (IGS) regions. Of these, four informative hotspot regions (trnH-psbA,ndhF-rpl32,trnK-rps16, andtrnP-psaJ) should be especially useful for future studies of phylogeny, phylogeography and conservation genetics of Menispermaceae.

A new study shows that Vanilla species are unique among orchids for having evolved a multimodal mechanism of seed and fruit dispersal. Bees inadvertently transport seeds externally while collecting fragrance or nest material from dehiscent fruits. Rodents and marsupials consume and disperse aromatic fleshy fruits and the crustose seeds within.

Species delimitation has long been a subject of controversy, and there are many alternative concepts and approaches used to define species in plants. The genus Amana (Liliaceae), known as "East Asian tulips" has a number of cryptic species and a huge genome size (1C = 21.48-57.35 pg). It also is intriguing how such a spring ephemeral genus thrives in subtropical areas. However, phylogenetic relationships and species delimitation within Amana are challenging. Here we included all species and 84 populations of Amana, which are collected throughout its distribution range. A variety of methods were used to clarify its species relationships based on a combination of morphological, ecological, genetic, evolutionary and phylogenetic species concepts. This evidence supports the recognition of at least 12 species in Amana. Moreover, we explored the complex evolutionary history within the genus and detected several historical hybridization and introgression events based on phylogenetic trees (transcriptomic and plastid), phylonetworks, admixture and ABBA-BABA analyses. Morphological traits have undergone parallel evolution in the genus. This spring ephemeral genus might have originated from a temperate region, yet finally thrives in subtropical areas, and three hypotheses about its adaptive evolution are proposed for future testing. In addition, we propose a new species, Amana polymorpha, from eastern Zhejiang Province, China. This research also demonstrates that molecular evidence at the genome level (such as transcriptomes) has greatly improved the accuracy and reasonability of species delimitation and taxon classification.

Geographical variation in species richness in plant groups is determined by the interplay between historical, evolutionary, and ecological processes. However, the processes underlying the striking disparity in species richness between Asia and the Americas remain poorly understood. Here, we synthesize global phylogenetic and macroecological data on the diversification of Smilacaceae, deciphering potential drivers underlying the species diversity pattern biased toward Asia. We compiled global distributions of all Smilacaceae species, and reconstructed the biogeographic history and niche evolution using a new time-calibrated phylogeny (eight genes, 135 species). Integrating these data sets, we estimated evolutionary histories and diversification rates for each region, and tested correlations among species diversification, niche evolution, and niche divergence. Smilacaceae probably originated during the Late Cretaceous/Early Palaeocene and began to diversify in middle to low latitudes in Central America and Eurasia during the Late Eocene. Both the Old and New World clades exhibited a steady, albeit slight, increase of species diversification from the Late Eocene to Early Miocene. However, the Old World clade experienced an abrupt increase in net diversification during the Late Miocene. Our findings also revealed that species diversification rates were positively correlated with ecological niche evolution and niche divergence. Niche shifts and climatic niche evolution since the Middle Miocene played crucial roles in species diversification dynamics within Smilacaceae. The high plant richness in Asia may be explained by greater diversification in this region, potentially promoted by heterogeneous environments.

Studies of the evolutionary relationships among gorilla populations using autosomal and mitochondrial sequences suggest that male-mediated gene flow may have been important in the past, but data on the Y-chromosomal relationships among the gorilla subspecies are limited. Here, we genotyped blood and noninvasively collected fecal samples from 12 captives and 257 wild male gorillas of known origin representing all four subspecies (Gorilla gorilla gorilla, G. g. diehli, G. beringei beringei, and G. b. graueri) at 10 Y-linked microsatellite loci resulting in 102 unique Y-haplotypes for 224 individuals. We found that western lowland gorilla (G. g. gorilla) haplotypes were consistently more diverse than any other subspecies for all measures of diversity and comprised several genetically distinct groups. However, these did not correspond to geographical proximity and some closely related haplotypes were found several hundred kilometers apart. Similarly, our broad sampling of eastern gorillas revealed that mountain (G. b. beringei) and Grauer's (G. b. graueri) gorilla Y-chromosomal haplotypes did not form distinct clusters. These observations suggest structure in the ancestral population with subsequent mixing of differentiated haplotypes by male dispersal for western lowland gorillas, and postisolation migration or incomplete lineage sorting due to short divergence times for eastern gorillas.

Vanilla andina, a new species belonging to subgen. Vanilla, is described and illustrated based on living material from Peru and Ecuador. The new species is similar to V. armoriquensis but can be easily distinguished by the prominent parallel callus, longitudinal narrow keels that extend above the middle of the lip and broadly obtuse to emarginate midlobe with crispate margins.

PremiseNumerous processes influence plant distributions and co-occurrence patterns, including ecological sorting, limiting similarity, and stochastic effects. To discriminate among these processes and determine the spatial scales at which they operate, we investigated how functional traits and phylogenetic relatedness influence the distribution of temperate forest herbs. MethodsWe surveyed understory plant communities across 257 forest stands in Wisconsin and Michigan (USA) and applied Bayesian phylogenetic linear mixed-effects models (PGLMMs) to quantify how functional traits and phylogenetic relatedness influence the environmental distribution of 139 herbaceous plant species along broad edaphic, climatic, and light gradients. These models also allowed us to test how functional and phylogenetic similarity affect species co-occurrence within microsites. ResultsLeaf height, specific leaf area, and seed mass all influenced individualistic plant distributions along landscape-scale gradients in soil texture, soil fertility, light availability, and climate. In contrast, phylogenetic relationships did not consistently predict species-environment relationships. Neither functionally similar nor phylogenetically related herbs segregated among microsites within forest stands. ConclusionsTrait-mediated ecological sorting appears to drive temperate-forest community assembly, generating individualistic plant distributions along regional environmental gradients. This finding links classic studies in plant ecology and prior research in plant physiological ecology to current trait-based approaches in community ecology. However, our results fail to support the common assumption that limiting similarity governs local plant co-occurrences. Strong ecological sorting among forest stands coupled with stochastic fine-scale interactions among species appear to weaken deterministic, niche-based assembly processes at local scales.

Background and Aims The East Asian-Tethyan disjunction pattern and its mechanisms of formation have long been of interest to researchers. Here, we studied the biogeographical history of Asteraceae tribe Cardueae, with a particular focus on the temperate East Asian genus Atractylodes DC., to understand the role of tectonic and climatic events in driving the diversification and disjunctions of the genus. Methods A total of 76 samples of Atractylodes from 36 locations were collected for RAD-sequencing. Three single nucleotide polymorphism (SNP) datasets based on different filtering strategies were used for phylogenetic analyses. Molecular dating and ancestral distribution reconstruction were performed using both chloroplast DNA sequences (127 Cardueae samples) and SNP (36 Atractylodes samples) datasets. Key Results Six species of Atractylodes were well resolved as individually monophyletic, although some introgression was identified among accessions of A. chinensis, A. lancea and A. koreana. Dispersal of the subtribe Carlininae from the Mediterranean to East Asia occurred after divergence between Atractylodes and Carlina L. + Atraetylis L. + Thevenotia DC. at similar to 31.57 Ma, resulting in an East Asian-Tethyan disjunction. Diversification of Atractylodes in East Asia mainly occurred from the Late Miocene to the Early Pleistocene. Conclusions Aridification of Asia and the closure of the Turgai Strait in the Late Oligocene promoted the dispersal of Cardueae from the Mediterranean to East China. Subsequent uplift of the Qinghai-Tibet Plateau as well as changes in Asian monsoon systems resulted in an East Asian-Tethyan disjunction between Atractylodes and Carlina + Atractylis + Thevenotia. In addition, Late Miocene to Quaternary climates and sea level fluctuations played major roles in the diversification of A tractylodes. Through this study of different taxonomic levels using genomic data, we have revealed an overlooked dispersal route between the Mediterranean and far East Asia (Japan/Korea) via Central Asia and East China.

Dear Editor, The Chinese bayberry(Morelia rubra Lour.,2n=2x=16)is an evergreen fruit tree native to southern China and the only domesticated species in family Myricaceae[1].Today M.rubra is widely cultivated in subtropical regions of China and has become an economically important fresh fruit with＞300 varieties and an annual production of 1.5 million tons[2,3].The aim of the current study was to reveal the domestication history of M.rubra using restriction site-associated DNA sequencing(RAD-seq)data.Seventy-eight individuals were sampled,including 44 landraces and improved cultivated varieties,19 wild individuals from the natural distribution ranges in southern China,14 from five other Morella species,and 1 from Comptonia.

Numerous processes influence plant distributions and co-occurrence patterns, including ecological sorting, limiting similarity, and stochastic effects. To discriminate among these processes and determine the spatial scales at which they operate, we investigated how functional traits and phylogenetic relatedness influence the distribution of temperate forest herbs. We surveyed understory plant communities across 257 forest stands in Wisconsin and Michigan (USA) and applied Bayesian phylogenetic linear mixed-effects models (PGLMMs) to quantify how functional traits and phylogenetic relatedness influence the environmental distribution of 139 herbaceous plant species along broad edaphic, climatic, and light gradients. These models also allowed us to test how functional and phylogenetic similarity affect species co-occurrence within microsites. Leaf height, specific leaf area, and seed mass all influenced individualistic plant distributions along landscape-scale gradients in soil texture, soil fertility, light availability, and climate. In contrast, phylogenetic relationships did not consistently predict species-environment relationships. Neither functionally similar nor phylogenetically related herbs segregated among microsites within forest stands. Trait-mediated ecological sorting appears to drive temperate-forest community assembly, generating individualistic plant distributions along regional environmental gradients. This finding links classic studies in plant ecology and prior research in plant physiological ecology to current trait-based approaches in community ecology. However, our results fail to support the common assumption that limiting similarity governs local plant co-occurrences. Strong ecological sorting among forest stands coupled with stochastic fine-scale interactions among species appear to weaken deterministic, niche-based assembly processes at local scales.