El RD 1620/2007 sobre el regimen juridico de la' reutilizacion de las aguas depuradas ineluye el metodo de Bailenger modificado para el analisis de huevos de nematodos intestinales, parametro requerido en la mayoria de los usos permitidos. Es un metodo sencillo pero que requiere una mayor evaluacion de sus limitaciones, asi como optimizarlo para aumentar en porcentaje de recuperacion de huevos. En este trabajo se propone optimizar el metodo usando huevos de Ascaris suum y Trichuris suis tenidos con cristal violeta. Se han analizado muestras en paralelo y comparado el metodo oficial con las modificaciones que obtenian mejores resultados. Una vez optimizado el metodo, se ha comprobado que la recuperacion pasa del 50% con el metodo oficial hasta casi el 70% con las modificaciones utilizando huevos sin tenir.

El control sanitario de los lodos de EDAR compostados para su uso agricola incluye la detenninacion de Salmonella y Eseheriehia eolio Este ultimo parametro puede determinarse por filtracion por membrana y cultivo, o bien mediante medios liquidos para obtener el Numero Mas Probable (NMP). Este articulo describe el protocolo para aplicar en muestras de lodos el metodo Colilert de la empresa Idexx, t6cnica que pennite obtener resultados en 18 horass (con Colilert 18). El laboratorio de Emasesa tiene validado y acreditado por ENAC este m6todo para analiar muestras de lodo compostado.

ABSTRACT Using a repetitive extragenic palindromic PCR (REP-PCR), we genotypically characterized strains causing nosocomial Acinetobacter baumannii infections and analyzed the source of bacteremia in 67 patients from an institution in which infections by this bacterium were endemic. Six different genotypes were found, including 21, 27, 3, 9, 3, and 4 strains. The probable source of bacteremia, according to clinical and/or microbiological criteria, was known in 42 patients (63%): respiratory tract ( n = 19), surgical sites ( n = 12), intravascular catheters ( n = 5), burns ( n = 3), and urinary tract ( n = 3). The definite source of bacteremia, according to REP-PCR, could be established in 30 (71%) out of the 42 patients with strains from blood and other sites; in these cases clinical and microbiological criteria for the source of bacteremia were thus confirmed. In the remaining 12 patients (29%) the probable source was refuted by the REP-PCR method. The definite sources of bacteremia according to genotype were as follows: respiratory tract in 13 patients (31%), surgical sites in 8 (19%), intravascular catheters in 4 (9%), burns in 3 (7%), and urinary tract in 2 (5%). A comparison of strains from blood cultures and other sites with regard to their REP-PCR and antimicrobial resistance profiles was also made. Taking the REP-PCR as the “gold standard,” the positive predictive value of antibiotype was 77% and the negative predictive value was 42%. In summary, the utility of the diagnosis of the source of nosocomial A. baumannii bacteremia using clinical and/or microbiological criteria, including antibiotyping, is limited, as demonstrated by REP-PCR.