The variability in the emergence process of different populations was confirmed for two Echinochloa crus-galli populations, one from Italy (IT) and the second from Norway (NO). Seeds were sown in 12 localities over Europe and the Middle East, and the emergence patterns of IT and NO were compared with those of several local populations at each location. Seeds of each population were sown in pots buried to the ground level. The base temperature (T-b) for emergence was estimated by (1) analysing logistic models applied to the field emergence of IT and NO, and (2) a germination assay set in winter 2020 at constant temperatures (8, 11, 14, 17, 20, 26, 29 degrees C) with newly collected seeds in 2019 from the same fields where IT and NO had previously been harvested in 2015. The logistic models developed for IT and NO in each location showed that the emergence pattern of IT was similar to that of the local populations in Poland, Italy, Spain, Turkey South and Iran, while NO fitted better to those in Sweden and Latvia. No germination was obtained for IT in a germination chamber, but the estimated T-b with the logistic model was 11.2 degrees C. For NO, the estimated T-b was 8.8 degrees C in the germination chamber and 8.1 degrees C in the field. Results suggest that adaptation to local environmental conditions has led to inter-population differences in T-b and parameter estimates of thermal-time models to predict the emergence of E. crus-galli should only be used for populations with similar climatic and habitat conditions.

Echinochloa crus-galli (L.) P. Beauv. is one of the most important weeds. It is distributed worldwide and has adapted to diverse habitats and climatic conditions. This study aimed to compare the emergence patterns of two populations of E. crus-galli from different environments at 11 locations across Europe and the Middle East. Seeds of the two populations were collected from maize in Italy and from spring barley in Norway and were then buried in soil in autumn 2015. In the spring of 2016, the soil was disturbed around the usual seedbed preparation date in each location and emergence was recorded. The soil was again disturbed a year later and emergence was recorded for a second season. Total emergence, the times of onset, end and to 50% emergence and the period between 25% and 75% of emergence were analysed by two-way ANOVA and principal components analysis. The Italian population showed a higher emergence than the Norwegian population in Southern locations, while the ranking was reversed in Northern locations. In almost all locations, a tendency to emerge earlier was recorded for the Norwegian population, but the periods from 25% to 75% emergence were similar for both populations. Total emergence, and the times of onset and end of emergence seemed to be mainly under genotypic (plus maternal) control, suggesting there were different temperature thresholds for seedling emergence in each population. Conversely, the duration of emergence seemed to be mainly under environmental control. This research confirms the high variability between populations and suggests the need to continue identifying key characteristics for the development of efficient models for seedling emergence in specific climates and/or latitudes.

There is a need both in organic farming and on farms using integrated pest management for non-chemical measures that control the perennial weed flora. The effect of mechanical weeding and fertilisation on perennial weeds, fungal diseases and soil structure were evaluated in two different experiments in spring cereals. Experiment I included six strategies. The first strategy was (1) without specific measures against perennial weeds. The other strategies encompassed one or two seasonal control measures; (2) rhizome/root cutting with minimal soil disturbance in autumn, (3) hoeing with 24 cm row spacing, (4) combined hoeing and disc harrowing in autumn, (5) 'KvikUp' harrowing in spring, and (6) 'KvikUp' harrowing in spring and autumn. Experiment II included factor (i) inter-row hoeing and (ii) fertilisation level. This experiment included the comparison between normal row spacing (12 cm) with weed harrowing versus double row spacing (=24 cm) in combination with inter-row hoeing and 4 fertilisation levels (50-200 kg N ha(-1)). In experiment I the strategies consisting of no or one direct weed control measure (1, 2, 3 and 5) clearly did not control the perennial weeds. The two seasonal control measures (4 and 6) gave a satisfactory weed control and highest crop yield. The combination of best weed control and no measured harmful effects on soil structure or increase of fungal diseases may explain the highest yields for these strategies. In Experiment II, hoeing and 24 cm spacing gave less perennial biomass compared to 12 cm spacing. Grain yields increased linearly with increasing nitrogen input. The study shows that both inter-row hoeing and weed harrows, are important elements in integrated pest management practice and organic farming. In addition, our results indicate that efficient mechanical weeding is possible without harmful effects in crop rotation consisting of various spring cereals as regards soil structure and plant health.

During the last two centuries, the North American common ragweed (Ambrosia artemisiifolia L.) invaded a large part of the globe. Local adaptation of this species was revealed by a common garden experiment, demonstrating that the distribution of the species in Europe could extend considerably to the North. Our study compares two populations of common ragweed (one from the native range and one from the invaded range) that differ in flowering time in the wild: the invasive population flowers earlier than the native population under non-inductive long-day photoperiods. Experiments conducted in controlled environments established that the two populations differ in their flowering time even under inductive short-day photoperiods, suggesting a change in autonomous flowering control. Genetic analysis revealed that early flowering is dominantly inherited and accompanied by the increased expression of the floral activator AaFTL1 and decreased expression of the floral repressor AaFTL2. Early flowering is also accompanied by reduced reproductive output, which is evolutionarily disadvantageous under long vegetation periods. In contrast, under short vegetation periods, only early-flowering plants can produce any viable seeds, making the higher seed set of late-flowering plants irrelevant. Thus, earlier flowering appears to be a specific adaptation to the higher latitudes of northern Europe.

The sustainable management of unwanted vegetation in agricultural fields through integrated weed control strategies requires detailed knowledge about the maternal formation of primary seed dormancy, to support the prediction of seedling emergence dynamics. This knowledge is decisive for the timing of crop sowing and nonchemical weed control measures. Studies in controlled environments have already demonstrated that thermal conditions and, to some extent, water availability during seed set and maturation has an impact on the level of dormancy. However, it is still unclear if this applies also under field conditions, where environmental stressors and their timing are more variable. We address this question for Alopecurus myosuroides in south-western Sweden. We quantified the effects of cumulated temperature and precipitation as well as soil water potential during the reproductive growth phase of Amyosuroides on primary seed dormancy under field conditions. Empirical models differing in focal time intervals and, in case of soil water potential, focal soil depths were compared regarding their predictive power. The highest predictive power for the level of primary dormancy of A.myosuroides seeds was found for a two-factorial linear model containing air temperature sum between 0 and 7days before peak seed shedding as well as the number of days with soil water potential below field capacity between 7 and 35days before peak seed shedding. For soil water potential, it was found that only the top 10cm soil layer is of relevance, which is in line with the shallow root architecture of A. myosuroides. We conclude that for this species the level of dormancy depends on the magnitude and timing of temperature and water availability during the reproductive growth phase. Water availability appears to be more important during maternal environmental perception and temperature during zygotic environmental perception.

Background and Aims Competitive crops are a central component of resource-efficient weed control, especially for problematic perennial weeds such as Elymus repens. Competition not only reduces total weed biomass, but denial of resources can also change the allocation pattern - potentially away from the underground storage organs that make perennial weeds difficult to control. Thus, the competition mode of crops may be an important component in the design of resource-efficient cropping systems. Our aim was to determine how competition from companion crops with different modes of competition affect E. repens biomass acquisition and allocation and discuss that in relation to how E. repens responds to different levels of light and nutrient supply.Methods Greenhouse experiments were conducted with E. repens growing in interspecific competition with increasing density of perennial ryegrass or red clover, or growing at three levels of both light and nutrient supply.Key Results Elymus repens total biomass decreased with increasing biomass of the companion crop and the rate of decrease was higher with red clover than with perennial ryegrass, particularly for E. repens rhizome biomass. A reduced nutrient supply shifted E. repens allocation towards below-ground biomass while a reduced light supply shifted it towards shoot biomass. Red clover caused no change in E. repens allocation pattern, while ryegrass mostly shifted the allocation towards below- ground biomass, but the change was not correlated with ryegrass biomass.Conclusions The companion crop mode of competition influences both the suppression rate of E. repens biomass acquisition and the likelihood of shifts in E. repens biomass allocation.

The invasive weed Ambrosia artemisiifolia (common ragweed) constitutes a great threat to public health and agriculture in large areas of the globe. Climate change, characterized by higher temperatures and prolonged vegetation periods, could increase the risk of establishment in northern Europe in the future. However, as the species is a short-day plant that requires long nights to induce bloom formation, it might still fail to produce mature seeds before the onset of winter in areas at northern latitudes characterized by short summer nights. To survey the genetic variation in flowering time and study the effect of latitudinal origin on this trait, a reciprocal common garden experiment, including eleven populations of A. artemisiifolia from Europe and North America, was conducted. The experiment was conducted both outside the range limit of the species, in Sweden and within its invaded range, in Croatia. Our main hypothesis was that the photoperiodic-thermal requirements of A. artemisiifolia constitute a barrier for reproduction at northern latitudes and, thus, halts the northern range shift despite expected climate change. Results revealed the presence of a north-south gradient in flowering time at both garden sites, indicating that certain European populations are pre-adapted to photoperiodic and thermal conditions at latitudes up to, at least, 60° N. This was confirmed by phenological recordings performed in a region close to the northern range limit, the north of Germany. Thus, we conclude that there exists a high risk for establishment and spread of A. artemisiifolia in FennoScandinavia in the near future. The range shift might occur independently of climate change, but would be accelerated by it.

Methods for control of couch grass (Elymus repens L.) with reduced tillage and cover crops to achieve low risk of nitrogen (N) and phosphorus (P) leaching were investigated. Treatments with reduced post-harvest tillage (one or two passes with duckfoot cultivator), hoeing between rows in combination with a cover crop, and a cover crop mown twice during autumn were compared with treatments with conventional disc cultivation and the control without tillage or cover crop. The study was conducted on a sandy soil in Sweden with measurements of N and P leaching. A 2-year experimental protocol was used, repeated twice. Treatments were implemented in the first year, and effects on couch grass (shoot density, shoot and rhizome biomass) were measured during autumn and in the second year. Significant effects of a single duckfoot cultivation and cover crop strategies were observed on couch grass shoot density in autumn but persistent effects were not verified. In conclusion, a single cultivation after harvest instead of repeated reduced the risk of N leaching and a cover crop in combination with hoeing or mowing effectively reduced it. Repeated cultivations resulted in mean annual N leaching of 26 kg N ha−1 compared with 20 kg in the treatment with one cultivation, 17 kg in the control, 16 and 12 kg in cover crop treatments with mowing and hoeing, respectively. The P leaching was small (0.04–0.09 P ha−1 year−1), but there were indications of increased P drainage water concentrations in the treatment with a cover crop which was mown.

Mechanical control through different tillage methods is a common measure against perennial weeds. However, non-optimal tillage can potentially increase the vegetative regeneration instead of reducing it. Aspects of regenerative capacity in the perennial weed species Rumex crispus L. was studied in three pot and box experiments as follows: (1) regeneration from different root fragments under varying test conditions; (2) effects of root size and burial depth on time of emergence and emergence rate; and (3) effects of burial depth and cutting on biomass production and seed production. In experiment 1, sprouting occurred mainly from the neck of the roots, consisting of underground stem tissue. A few shoots were observed also from the upper half of the true taproot and from a side root. No difference in sprouting was found between test conditions. In experiment 2, total emergence rate was positively related to root size and negatively related to burial depth. Time to first emergence was defined by an interaction between the two factors. Roots larger than 100 g gave rise to a high degree of emergence from all burial depths, while emergence from roots weighing 20-30 g was less than 30% from 12 cm and nonexistent from 18 cm. When emergence and shoot production were tested under different cutting regimes (experiment 3), the separated top fragments of the roots sprouted faster and produced more shoot biomass than intact rootstocks, even at a second harvest. Our results indicate that fragmentation can stimulate sprouting from the regenerative tissue of the taproot. Hence, if tillage is carried out as weed control it is important that root fragments are buried at ploughing depth, especially if the population consists of adult plants.

SPROUTING CAPACITY OF UNDERGROUND LATERAL BUDS OF RHIZOMATEOUS PERENNIAL WEEDS VARY WITH SEASON

Seasonal variation in sprouting capacity from underground root buds of Cirsium arvense and Sonchus arvensis

Spirehvile i rodder og rhizomer i de vigtigste rodukrudtsarter - resultater fra svenske forsog.

Emergence pattern of the perennial weed species Rumex crispus L. was studied under semi-field conditions. Seeds from three populations were harvested from the mother plants and sown in pots buried in an experimental field, either in late autumn or after winter storage at outdoor temperatures. Seeds were sown on the soil surface or covered by a 2-cm soil layer. In addition, some of the seeds sown in autumn were also subjected to mechanical disturbance in autumn or in spring. Population and soil cover both had a strong effect on total emergence. The light requirement of the species for germination was indicated by a higher rate of emergence from seeds sown on the soil surface than from covered seeds. Sowing date influenced the timing of emergence but not the total emergence. Autumn sowing led to earlier and more concentrated emergence, while seedlings from spring-sown seeds showed a more intermittent emergence pattern. Stirring after sowing had a positive effect on emergence compared with emergence from undisturbed, covered seeds. In all populations and treatments, emergence continued throughout the growing season, contradicting earlier findings that R. crispus seeds germinate mainly in early spring and autumn, and enter secondary dormancy during summer. The delay of emergence in seeds that had remained on the mother plant over winter indicates a certain level of dormancy, which was gradually broken in early summer.

Reduced bud sprouting from intact roots and rhizomes of perennial weeds in Sweden during autumn.

Variation in primary dormancy among populations of black nightshade and hairy nightshade collected on two dates was investigated. In addition, emergence characteristics of both species were Studied during 2 yr with several populations and soil disturbance regimes. Results revealed a variation in the level of primary dormancy among populations and collection dates in black nightshade. However, fresh seeds of hairy nightshade showed no or negligible germinability, indicating a deeper level of dormancy. Emergence was higher in black nightshade than in hairy nightshade in both years, but with significant differences among populations. In addition, various categories regarding the soil temperature requirements for seedling emergence were found within seed batches. This enables the species to extend their emergence timing and therefore escape from climatic extremes or weed control operations. This information can be used to improve weed management strategies by timing weed control Measures to larger seedling flushes.