Cellular metabolism in tumor is an important biological process to promote the occurrence and development of tumor, and the genes related to cellular metabolism are gradually becoming the targets of tumor treatment. However, more researches are still needed to explore the abnormal metabolism in tumor progression and its prognostic value. BDH2 gene is a multifunctional gene, participates in a variety of metabolic pathways, plays an important catalytic role in iron metabolism, participates in ketone metabolism and is related to lipid metabolism. In recent years, researchers have found that BDH2 plays distinct roles in various types of tumors. The occurrence and development of a variety of tumors are closely related to BDH2. This paper analyzes, summarizes and prospects the role and mechanism of BDH2 in metabolism and tumorigenesis.

[目的]探索二十二碳六烯酸(DHA)对高脂饲粮诱导肝脏脂肪积累的预防机制.[方法]将32只雄性SPF级C57BL/6小鼠均分为4组,对照组(Con)饲喂普通饲粮,模型组(Model)饲喂高脂饲粮,DHA组分别在高脂饲粮中添加0.2 g DHA(DHAL)和1.0 g DHA(DHAH),饲喂周期为20周.饲喂期间每天称量体重及食物重量,计算摄食量;饲喂结束,采集肝脏和血液,ELISA法检测肝脏脂联素和血清甘油三酯(TG)的含量,实时荧光定量PCR检测新生脂肪合成关键酶(SREBP-1c、FAS)、脂肪酸氧化关键基因(PPARα、PPARγ、CPT-1A和ACOX)、线粒体基因(PGC-1α)、褐色脂肪化基因(Prdm16、UCP1)的表达,Western blotting法检测肝脏磷酸化ACC、AMPK和AKT蛋白的表达.[结果]与Con组相比,Model组终体重、体脂重量和TG含量均显著增加(P＜0.05),肝脏脂联素浓度显著降低(P＜0.05).与Model组相比,DHAL和DHAH组终体重、体脂重量和TG含量均显著降低(P＜0.05),肝脏脂联素水平显著增加(P＜0.05).实时荧光定量PCR结果表明,与Con组相比,Model组SREBP-1c和FASmRNA表达量均显著增加(P＜0.05),PPARα、CPT-1A、ACOX、PGC-1α和UCP1 mRNA表达均显著降低(P＜0.05);与Model组相比,DHAL和DHAH组SREBP-1c和FAS mRNA表达量均显著降低(P＜0.05),且DHAH 组表达量均显著低于 DHAL 组(P＜0.05),DHAL 和 DHAH 组 PPARα、CPT-1A、ACOX、PGC-1α、Prdm16和UCP1 mRNA表达量均显著增加(P＜0.05),DHAH组CPT-1A和ACOX mRNA表达量显著低于DHAL组(P＜0.05),PPARγ的mRNA表达量在4组中没有显著差异(P＞0.05).Western blotting结果表明,Model组p-ACC显著高于Con和DHAH组,但显著低于DHAL组;Model组p-AMPK/AMPK和pAKT/AKT比值均显著低于 Con 组(P＜0.05),DHAH 组 p-AMPK/AMPK 和 pAKT/AKT 比值均显著高于 Model 和 DHAL 组(P＜0.05).[结论]DHA可降低高脂饲粮导致的C57BL/6小鼠终体重、体脂和TG含量的升高,增加肝脏脂联素的水平,促进脂肪酸氧化和白色脂肪细胞褐色化,从而预防肝脏的脂肪积累.