The objective of this study was to explore the protection effects of NADH on Cardiac injury Induced by ex cessive exercise in vivo.Kunmin mice were divided four groups:control group and cardiac injury group,NADH con trol group,pretreatment with NADH before excessive exercise group.After the excessive exercise session,effect of NADH on the mice body weight,SOD activation and MDA level,the mitochondria membrane potential and the rate of apoptosis and necrosis were studied.It revealed that excessive exercises could induce SOD activation and MDA level increase.Mitochondria membrane potential decreased.The rate of apoptosis and necrosis increased.NADH could decrease SOD activation and MDA level,increase mitochondria membrane potential,and decrease the rate of apoptosis and necrosis induced by excessive exercise cardiac injury.The results showed that NADH pretreatment could reduce the oxidative injury and apoptosis induced by excessive exercise.

Concentrations,pollution sources and distribution characteristics of heavy metals(Cr,Zn,Cu,Ni,Pb,Cd,Fe,Mn) were investigated in the influent,effluent and activated sludge of a tannery and textile dyeing industrial park wastewater treatment plant in Zhejiang,based on which the influence of heavy metals on the pollutant removal performance was discussed.Results showed that the highest contents of heavy metals in the influent were Cr and Fe,respectively reaching 16.40 mg/L and 15.80 mg/L.These heavy metals accumulated in the activated sludge,with Cr and Fe as high as 12550 mg/kg-dried sludge and 13671 mg/kg-dried sludge.The enrichment of heavy metals in the activated sludge not only brought difficulties in the sludge disposal and resource utilization,but also gave negative impact on the solid-liquid separation of sludge and the pollutants removal performance.