通过单因素试验和正交试验,对人参锈腐病拮抗细菌HN01进行摇床发酵条件和培养基配方研究.结果表明:该菌最适发酵条件为温度30℃,初始pH值7.0,250mL三角瓶装液50mL,接菌量10％,摇床转速150r· min-1,发酵时间36h.最佳产菌培养基组分为葡萄糖15.0g·L-1,牛肉膏8g·L-1,NaCl 5g·L-1,(NH4)2SO41 mg· L-1,K2HPO4 4g·L-1.抑菌培养基组分为葡萄糖10g·L-1,牛肉膏8g· L-1,NaCl3g· L-1,(NH4)2SO4 2mg· L-1,K2HPO4 4g· L-1

2010年对沈阳地区稻曲病病菌孢子捕捉发现,稻曲病病菌分生孢子始见于7月下旬,飞散期为7～9月。其中8月为飞散盛期,9月初进入消退期。自捕捉到孢子20 d后田间开始陆续发病。稻曲病分生孢子飞散动态与积温呈极显著正相关(r=0.822,Sig=0.007<0.01),与积累降雨量呈显著性正相关(r=0.774,Sig=0.014<0.05),与田间病情扩散动态呈显著正相关。

To investigate occurrence and control technology of windflower leaf spot,the incidence of leaf spot on windflower in Liaoning was investigated by five point sampling and the method of combining large census.It was found that occurrence of windflower leaf spot caused by Ascochyta anemones Kab.et Bub.was closely to cultivated variety,plant age and cultivation pattern.The diameter of the spot was coincided with the field disease trends.This disease could be effectively controlled by procymidone 50%WP,pyrimethanil 40%SC,azoxystrobin 25% SC at its early stage.