We aimed to identify the potential genes related to blood pressure regulation and screen target genes for high blood pressure (BPH) and low blood pressure (BPL) treatment. The GSE19817 microarray dataset, which included the aorta, liver, heart, and kidney samples from BPH, BPL, and normotensive mice, was downloaded from the Gene Expression Omnibus. Principal component analysis (PCA) was performed based on the entire expression profile. Differentially expressed genes (DEGs) were screened, followed by pathway enrichment analysis. Finally, gene regulatory networks were constructed based on BPH-related and BPL-related DEGs in the aorta, liver, heart, and kidney samples. As a result, DEGs were screened within their respective tissues due to high heterogeneity of different tissues. Totally, 2,726 BPH-related DEGs and 2,472 BPL-related DEGs were screened, which were mainly enriched in pathways such as immune response. The topology data of gene regulatory networks constructed by DEGs in the heart, kidney, and liver were similar than that in aorta. Finally, among BPH-related DEGs, Sept6 and Pigx were found in the top 10 differentially regulated DEGs by comparing the BPH-related DEGs of the aorta with the DEGs of the other 3 tissues in the regulatory network. Although among the top 10 differentially regulated BPL-related DEGs, no common differentially regulated DEGs were found, Wif1, Urb2, and Gtf2ird1 were found among the top ten DEGs in the three tissues other than the kidney tissue. Sept6 and Pigx might participate in the pathogenesis of BPH, whereas Gtf2ird1, Urb2, and Wif1 might be critical target genes for BPL treatment.

OBJECTIVE:To examine the relationship between single nucleotide polymorphism (SNP) of adiponectin (APN) locus +45T/G and Han male patients with premature coronary artery heart disease (pCAD).METHODS:A total of 423 male patients of Han ethnic group (< 55 yr old) undergoing coronary arteriography were recruited. Among them, 358 patients were diagnosed as pCAD while another 65 normal control (NC). All subjects were genotyped for adiponectin gene SNP +45 by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.RESULTS:Statistical differences of TG/GG genotype ratio were observed between two groups. The TG/GG genotype ratio in the pCAD group was 51.1% versus 35.4% in the NC group (χ(2) = 5.45, P = 0.022). After an adjustment of conventional risk factors, such as age, body mass index (BMI) and hypertension, the adiponectin gene SNP +45TG/GG genotype was strongly associated with Han ethnic group male pCAD by binary logistic regression analysis (OR = 1.843, P = 0.035, 95%CI: 1.045 ∼ 3.250). And there were statistical differences between TT genotype and TG/GG genotype among pCAD subjects in the following factors: BMI, total cholesterol (TC), low-density lipoprotein-C (LDL-C) and APN level (P < 0.05). By Pearson correlation analysis, APN was negatively correlated with TC, LDL-C and SNP +45T/G polymorphism.CONCLUSION:The SNP of adiponectin locus +45T/G is associated with male pCAD. And TG/GG genotype is a possible predisposing gene for Han ethnic group male pCAD.

目的探讨冠状动脉粥样硬化患者血浆瘦素、脂联素水平及其与动脉粥样硬化的关系。方法选择242例冠状动脉粥样硬化患者,按冠脉造影结果分为对照组(冠脉狭窄<50%)107例、冠心病(CHD)组135例,分别检测两组患者血浆瘦素、脂联素水平,并分析其与动脉粥样硬化的关系。结果 CHD组血浆脂联素水平[(4.81±0.96)mg/L]显著低于对照组[(6.04±0.98)mg/L](P<0.01);血浆瘦素水平[(1.71±0.36)μg/L]显著高于对照组[(1.32±0.31)μg/L](P<0.01)。Pearson相关分析显示,血浆瘦素与脂联素水平呈显著负相关(r=-0.672,P<0.01),且随着Gensini积分增高,血浆瘦素水平逐渐增高,而脂联素水平逐渐下降(P<0.01)。结论冠状动脉粥样硬化患者冠脉病变程度与血浆瘦素和脂联素水平均有相关性;血浆瘦素与脂联素水平存在相互抑制调节的关系。

OBJECTIVE:To investigate the effects of carvedilol and metoprolol on the expression of autoantibodies against cardiac β(1), β(2) and α(1) adrenergic receptors in aged patients with chronic heart failure (CHF) and ventricular arrhythmia (VA).METHODS:Sixty-eight patients with CHF and VA were randomly divided metoprolol treatment group or carvedilol treatment group on the basis of digoxin and diuretic treatment. All patients were followed up for six months cardiac function was monitored by echocardiography, VA by Holter and the three autoantibodies by enzyme-linked immunosorbent assay (ELISA).RESULTS:(1) Systolic blood pressure and brain natriuretic peptide (BNP) were significantly lower in carvedilol group than that in metoprolol group (P < 0.05). (2) The positive ratio of autoantibodies against the cardiac β(1) adrenergic receptor was significantly decreased compared with that of pre-treatment (P < 0.05) in metoprolol group. The positive ratios of autoantibodies against cardiac β(1), β(2) and α(1)-adrenergic receptors were all significantly decreased compared with that of pre-treatment (P < 0.01) in carvedilol group. Moreover, the incidence of VA was significantly decreased in carvedilol group (P < 0.05) but not in metoprolol group.CONCLUSION:Carvedilol is superior to metoprolol on decreasing the incidence of VA in aged patients with chronic heart failure and ventricular arrhythmia.