"民以食为天,食以安为先",食品作为农产品的主要产品,它的需求变化对整个农业发展意义重大,它的安全问题关系着人民群众的身体健康、生命安全和社会的稳定。从本期开始依次为您解读《北京市食品安全条例》中农产品质量安全管理内容。

Forty five 6-week-old Kunming mice were divided randomly into three groups,one time inoculated group（group A） and consecutive inoculated group（group B） and control group（group C）（each group,n=15）.The group A were inoculated with PRRSV and PCV-2 in the first day,group B were inoculated with PRRSV and PCV-2 in the 1 st,3th,5th d,group C were inoculated with MEM cell culture medium in the first day.Three mice of three groups were executed after the 7th,14th,21th,28th,35th d.Body weight measurement,antibody detection,fluorescent quantification PCR detection,naked eye and micro-pathology observation were used to detect the treated animals.The results showed that compared with group C,the group A and B displayed slow weight gain in mice,typical lung lesions of interstitial pneumonia,inguinal lymph nodes swelling,lymphocyte necrosis,spleen lymphocytes depletion,scattered subcapsular hemorrhage,the liver venous stasis,antibody positive.Fluorescent quantification PCR testing confirmed the existence of viral replication,except the group C.The relative amount of virus in lymph nodes was more than that of other tissues（P0.01）.So the diseased model could be established successfully in Kunming mice coinfected with PRRSV and PCV-2.