Muscadine wine has a unique polyphenol profile distinct from that of common wine, and limited research exists on its health benefits. This study aimed to investigate the effects of intake of dealcoholized muscadine wine (DMW) on skin health, oxidative stress, inflammatory biomarkers, and the gut microbiome. Seventeen healthy women were randomly assigned to consume 300 mL of DMW or a placebo daily for 6 weeks, separated by a 3-week washout period, in a randomized, single-blinded, crossover design. Skin health parameters were measured on the face and forearm. Oxidative stress and inflammatory biomarkers were assessed in plasma. Fecal bacterial DNA was sequenced using shotgun sequencing. DMW did not affect UVB-induced erythema compared to placebo. However, it significantly decreased transepidermal water loss and increased facial gross elasticity. Skin elasticity significantly improved on the forearm, whereas other skin parameters were not affected. DMW significantly decreased plasma levels of matrix metalloproteinase-9 and advanced glycation end products compared with placebo. However, the abundance, diversity, and functions of the gut microbiome were not affected. Polyphenol-rich DMW administered for six weeks improved certain skin health parameters and reduced oxidative and inflammatory stress, without affecting the gut microbiome in healthy women.

Wines are traditionally packaged in glass bottles, but recent advancements in the can liner technology have made cans more practical and economically appealing for wineries. However, limited research has been published on how packaging affects muscadine (Vitis rotundifolia) wine quality. This project investigated the chemical changes in carbonated red muscadine wine packaged in aluminum cans and glass bottles at accelerated and ambient temperatures (35.9 degrees C/24.8 degrees C). Across both packaging types, there was a 54% reduction in the anthocyanin concentration (4506 mg/L to 2096 mg/L) over the ambient storage period, which followed a temperature coefficient (Q 10) of 1.83. Most analyses had no significant differences between packaging types, notably volatile organic compounds and anthocyanins. Wine color measured by a colorimeter (Delta E2000) showed no correlation over time, suggesting undetectable differences for casual consumers. These findings suggest that wine packaged in bottles and cans exhibits comparable chemical changes over time, supporting cans as a viable alternative package.

Human skin health deteriorates after age 40, particularly due to ultraviolet (UV) radiation, a major extrinsic factor contributing to photoaging. This study investigated whether daily consumption of 100% orange juice could reduce UVB-induced erythema, improve skin health, and lower biomarkers of oxidative stress and inflammation. A randomized, single-blinded, crossover trial was conducted in 24 healthy women aged 40–65. Participants consumed 12 oz of 100% orange juice or an orange-flavored control beverage daily for 10 weeks, followed by a 28-day washout before switching beverages for another 10 weeks. Skin assessments and blood sample collection were conducted at baseline, week 5, and week 10. Orange juice offers significantly higher daily flavanone content (81.46 mg/day) than the control beverage (29.60 mg/day). Ten weeks of orange juice consumption significantly reduced forearm wrinkles and showed a trend toward reduced skin roughness. However, no significant improvements were observed in UVB-induced erythema and other skin health parameters, including transepidermal water loss, skin hydration, or elasticity. Furthermore, blood levels of matrix metalloproteinase-9, advanced glycation end products, superoxide dismutase, glutathione peroxidase, IL-6, TNF-α, and high-sensitivity C-reactive protein remained unchanged. In conclusion, daily consumption of orange juice for 10 weeks had a marginal effect on skin health.

Huang long bing (HLB) infection of oranges induces the biosynthesis and accumulation of bitter limonoids. The objective of this study was to debitter HLB-affected orange juice while preserving the tasteless and health-promoting limonoid glucoside and flavanones using resin adsorption. Three resins (FPX66, PAD900, and XAD16N) were found to have higher adsorption and desorption capacity for limonin among seven selected resins. Adsorption of limonoids rapidly increased in the first 2 h of kinetic tests, while slower adsorption kinetics were observed for flavanones. Limonin isothermal adsorption fit best with the Langmuir model, suggesting a physical, monolayer process. Dynamic adsorption showed that a fixed-bed column packed with PAD900 was able to debitter 200 bed volumes of orange juice before limonin reached its taste threshold of 4.7 µg/mL in the eluent. The resin was regenerated by eluting the column with four bed volumes of alcohol or 4% NaOH to remove adsorbed compounds from the resin. Resin adsorption reduced limonin content in orange juice from 5.85 to 1.34 µg/mL but also decreased tasteless compounds by at least 40%. A consumer sensory test gave the debittered juice a higher overall liking and preference over untreated juice. This study showed that resin adsorption is an effective approach to reduce the bitterness of orange juice affected by HLB.

Background/Objectives: Colitis is a chronic condition affecting millions worldwide. Purple muscadine wine polyphenols have a unique composition and possible disease-preventive properties. This study aims to determine how dealcoholized muscadine wine (DMW) affects the development of colitis and gut microbiome in IL-10−/− mice, compared to wild types (WT). Methods: Six-week-old male IL-10−/− and WT C57BL/6 mice were fed either a DMW-supplemented diet (4.8% v/w) or a control diet based on AIN-93M for 154 days. Colitis severity was evaluated by disease activity, intestinal permeability, gene expression of cytokines and tight junction proteins in the colon, and inflammatory cytokines in the serum. Fecal samples were collected for gut microbiome profiling via 16S rRNA gene sequencing. Results: DMW contained predominantly anthocyanins and a significant amount of ellagic acid. IL-10−/− mice developed mild colitis as indicated by the disease activity index. DMW × gene interactions decreased intestinal permeability, colonic mRNA levels of IL-1β, and serum TNF-α in the IL-10−/− mice. DMW suppressed the colonic mRNA levels of IL-6, enhanced the gene expression of ZO-1, but did not influence the mRNA level of TNF-α or occludin. While DMW did not alter α-diversity of the gut microbiome, it significantly influenced β-diversity in the WT mice. DMW significantly reduced the relative abundances of Akkermansia in the IL-10−/− and WT mice. DMW and DMW×gene interaction decreased the relative abundance of Parasutterella only in IL-10−/− mice. Conclusions: These results suggested that polyphenols from DMW interacted with genes to moderately alleviate the development of colitis in IL-10−/− mice and could be a useful dietary strategy for IBD prevention.

This study aimed to determine whether a polyphenol-rich cranberry beverage affects skin properties, lipids, and the microbiome in women using a randomized, double-blinded, placebo-controlled, cross-over design. Twenty-two women with Fitzpatrick skin types 2–3 were randomized to drink a cranberry beverage or placebo for six weeks. After a 21-day washout, they consumed the opposite beverage for six weeks. Six weeks of cranberry beverage significantly reduced UVB-induced erythema, improved net elasticity on the face and forearm, smoothness on the face, and gross elasticity on the forearm compared to the placebo. When stratified by age, these effects of the cranberry beverage were primarily observed in women >40 years old. SOD activities were improved after six weeks of cranberry beverage consumption compared to the placebo, while glutathione peroxide and TNF-α were improved compared to baseline. These effects were found to differ by age group. Skin lipid composition was modulated by both the cranberry beverage and the placebo. Cranberry beverages did not change α- or β-diversity but altered the abundance of several skin microbes at the species and strain level. Consumption of a cranberry beverage for six weeks improved specific skin properties and oxidative stress and modulated skin lipids and microbiome compared to placebo.

Retraction of 'Dealcoholized muscadine wine was partially effective in preventing and treating dextran sulfate sodium-induced colitis and restoring gut dysbiosis in mice' by Hao Li et al., Food Funct., 2023, 14, 5994-6011, https://doi.org/10.1039/D3FO00047H.

Current trends have been noticed in to study of unedible parts of food as sources of phytochemicals or as ingredients in order to develop new uses for discarded materials and to promote sustainability. This study aimed to evaluate the performance of six macroporous resins (XAD 16, XAD 7HP, XAD 2, XAD 4, DAX 8, and XAD 1180 N) in purification of phenolic compounds from organic purple-fleshed sweet potatoes. Static adsorption and desorption showed that XAD16 and XAD7HP had the highest adsorption and desorption ratio and recovery yield. The process temperature did not affect the adsorption capacity of XAD16. However, XAD7HP showed higher adsorption capacity when performed at 30 degrees C, compared with 40 and 50 degrees C. The process of equilibrium adsorption isotherms for phenolics was better described using Freundlich isotherm model. Dynamic adsorption process on XAD7HP resin showed that phenolic compounds in the water extracts of leaves started to break through after 4.5 bed volumes of extract was loaded. The purification of phytochemicals using adsorption is an alternative to replace organic solvents and provide a safety process for industrial application.

Intake biomarkers of cranberry juice in women can assess consumption in clinical trials. Discriminant biomarkers in urine may explain urinary tract infection (UTI) preventive activities. We hypothesized that validated and annotated discriminant metabolites in human urine could be used as intake biomarkers in building predictive multivariate models to classify cranberry consumers. Urine samples were collected from 16 healthy women aged 18 to 29 years at baseline and after 3- and 21-day consumption of cranberry or placebo juice in a double-blind, crossover study. Urine metabolomes were analyzed using ultra high-performance liquid chromatography coupled with Orbitrap mass spectrometry. Paired and unpaired multivariate analyses were used to annotate or identify discriminant metabolic features after cranberry consumption. Twenty-six discriminant metabolic features (paired analysis) and 27 (unpaired analysis) after cranberry consumption in an open-label intervention were rediscovered in the blinded study. These metabolites included exogenous (quinic acid) and endogenous ones (hippuric acid). The paired analysis showed better model fitting with partial least-square discriminant analysis models built on all metabolites than the unpaired analysis. Predictive models built on shared metabolites by the unpaired analysis were able to classify cranberry juice consumers with 84.4% to 100% correction rates, overall better than the paired analysis (50%-100%). The double-blind study validated discriminant metabolites from a previous open-label study. These urinary metabolites may be associated with the ability of cranberries to prevent UTIs and serve as potential cranberry intake biomarkers. It reveals the importance of selecting the right predictive models to classify cranberry consumers with higher than 95% correction rates.

Six new NDES (#1-6) were designed to extract polyphenols in olive leaves in comparison to 7 previously reported ones (#7-13) including aqueous 75 % ethanol. Artificial neural net-working (ANN) was used to model the extraction parameters and predict the extraction yield. Twelve phenolic compounds were identified and quantified in leaf extract using HPLC-MS. NDES #3 extracted the highest yield of rutin (4.84 mg/g), luteolin 7-O-glucoside (8.36 mg/g), luteolin 4 '-O-glucoside (5.42 mg/g), luteolin (23.10 mg/g), apigenin (0.52 mg/g), and chrysoeriol (0.32 mg/g). NDES #4 extracted higher amount of flavones and rutin from the olive leaves than 75 % ethanol. NDES #10 showed selectivity to extracted hydro-xytyrosol. The ANN optimization of the extraction parameters using NDES #3 increased the summed yield of flavones and rutin. This study demonstrated that newly designed NDES are more effective than previous ones and 75 % ethanol to extract major phenolic compounds in olive leaves.(c) 2023 Institution of Chemical Engineers. Published by Elsevier Ltd. All rights reserved.