An enriched glucan synthase fraction was obtained from red beet root microsomes by sequential extraction with the detergents 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate and digitonin. The digitonin suspension was centrifuged on a glycerol gradient, where a glucan synthase peak with a specific activity of 30- to 40-fold over microsomes was recovered. Most protein contaminants were found in the gradient pellet. The glucan synthase-containing fraction was largely free of plasma membrane and tonoplast-derived ATPase activity and was enriched with a protein subunit of 68 kilodaltons.

UDP-glucose: 1,3-1,4 ß-glucan synthase was solubilized from red beet (Beta vulgaris L.) root microsomes by treatment with 1% digitonin at 30°C. The enzyme shows unusual stability for a solubilized glucan synthase, with full activity retention after storage for 1 week at 4°C.

ADVERTISEMENT RETURN TO ISSUEPREVArticleGlucan biosynthesis in red beet root microsomes and tissue slicesBruce P. Wasserman, Carol L. Ventola, and Laura L. EibergerCite this: J. Agric. Food Chem. 1985, 33, 1, 144–149Publication Date (Print):January 1, 1985Publication History Published online1 May 2002Published inissue 1 January 1985https://pubs.acs.org/doi/10.1021/jf00061a040https://doi.org/10.1021/jf00061a040research-articleACS PublicationsRequest reuse permissionsArticle Views42Altmetric-Citations5LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts

ABSTRACTThe effect of hydrogen peroxide and phenolic compounds on the decolorization of betanin and a betaxanthin preparation by horse‐radish peroxidase (HRP) was examined. Betanin was decolorized at a greater rate than the betaxanthm pigments and both reactions were H2O2‐dependent. Betaxanthin was more prone to oxidatic decolorization than betanin. 2,4‐Dichlorophenol, resorcinol and o‐toluidine stimulated the decolorization of both pigments. Guaiacol enhanced the peroxidatic decolorization of both pigments to a small extent, but inhibited the oxidatic breakdown of betaxanthin. Possible implications of these results are discussed.