Introduction: The aim was to investigate the activity levels and changes in inflammatory markers during weekday supervised treatment and independent weekend treatments. This is the first study to examine any relationship between supervised and unsupervised activity over first week of an inpatient stay. Methods: Subjects were recruited and consented to complete the Habitual Activity Estimation Scale (HAES) on a weekday and weekend day. Inflammatory markers (CRP, WCC, Neutrophils) recorded on weekday and subsequent Monday during May − September 2011. Results were analysed using paired t-test for statistical significance. Results: 44 patients were included in the study. M:F 27:17. Significant reduction in CRP was displayed over the weekend period P = 0.018 no other inflammatory marker displayed statistical significance. No statistical significance was observed in HAES during the study period. On average 48% of subject’s day is inactive, while 22% of the day is spent in activity 3.8% of which is considered exercise. Conclusions: Expected reduction in CRP was displayed on commencement of treatment. No significant difference in activity was demonstrated. It is the conclusion of these authors that programmes set to maintain activity during weekends has proven clinical benefits. Overall this patient group display high levels of inactivity, further education is required to promote activity as part of standard care. 214 Core competencies for physiotherapists working with cystic fibrosis patients in Italy C. Tartali1, S. Zuffo2, F. Alatri3, A. Brivio4, M. Dona5, L. Graziano6. 1Azienda Ospedaliera Universitaria Integrata Verona, Centro Regionale Fibrosi Cistica, Verona, Italy; 2Azienda Ospedaliero-Universitaria Meyer, Rehabilitation, Firenze, Italy; 3Policlinico Umberto I Rome, Centro Fibrosi Cistica, Roma, Italy; 4IRCCS Policlinico Ca Granda Milan, Centro Fibrosi Cistica, Milano, Italy; 5Ospedale Ca’ Foncello ULSS 9, Treviso, Italy; 6Policlinico Umberto I Roma, Centro Fibrosi Cistica, Roma, Italy

Introduction: Responsiveness to exercise has been shown to be similar in CF and healthy untrained children. Higher levels of aerobic fitness in CF may impact on survival. As part of a pilot exercise and nutrition programme, CPET was undertaken to determine pre-intervention aerobic capacity and to develop individualised exercise prescriptions to improve levels of fitness. Objectives: To measure changes in VO2Peak in response to a regular intensive exercise programme. Methods: 12/16 children (12 female; 4 male; median age 11 [5−15] years) were eligible to complete CPET (Godfrey cycle protocol; work rate 10, 15 or 20W) at baseline and 12-months. FEV1%pred, SpO2 and ECG were monitored at each test session. Based on pre-intervention CPET results, children undertook 1−2 weekly individually prescribed exercise sessions with aerobic, anaerobic, strength and coreconditioning components, over a 12-month period. Dietetic adaptations were made in response to increased metabolic demand. Results: 2/12 children had a low (VO2Peak%pred 58%) and 10/12 had a medium (VO2Peak%pred = 59−81%) initial fitness level. Post intervention, there was a significant mean increase of 4.8ml×kg×min−1 in VO2Peak (p = 0.02) and a 13% change in VO2peak%pred (p = 0.03). VE/VCO2 decreased by 0.6 and FEV1 increased by 0.4 SDS indicating a non-significant trend towards improvement in ventilatory efficiency. All children maintained SpO2 >95% during testing and no arrhythmias were detected. Conclusion: The results of this pilot programme demonstrated that significant increases in aerobic capacity can be achieved with regular intensive exercise in children with CF, with a previously low-medium level of exercise capacity. WS16.10 Correlation between different tests to assess exercise capacity in patients with cystic fibrosis (CF) M. Dona1, G. Mamprin1, M. Vecchiato1, L. Da Dalt2, M. Ros1. 1Treviso Hospital, Cystic Fibrosis Centre, Treviso, Italy; 2Treviso Hospital, Pediatrics Department, Treviso, Italy

Background: Hypersecretion of viscous infected mucus plugging, mucosal inflammation, bronchoconstriction, airway instability or lung tissue damage in CF patients cause pulmonary obstruction.Although chest physiotherapy techniques are commonly used in CF respiratory diseases treatment, there are no studies evaluating the effect of the expiratory flow increase technique (French technique) on pulmonary function of CF patients.Aim: to evaluate the immediate effect of the EFIT on spirometric and cardio respiratory parameters in CF patients colonized by P aeruginosa with acute pulmonary exacerbation CAPE). Patients and Methods: A clinical, prospective study including 27 CF patients colonized by P aeruginosa at the CF center of State University of Campinas School Hospital, Brazil were studied.The patients were submitted to spirometry, SaO 2 measured by pulse oximetry, clinical examination and respiratory physiotherapy (EFIT) immediately before the treatment by intravenous antibiotics (step I).The same procedure was done two weeks later in all patients (step II).Results: 27 patients (17 females), aged 7 28 years (15±6.07)completed the study.15 patients were clinically severe by Shwachman Score.The mean values of variables from step I and step II were: oxygen saturation (92.15±6.06vs 93.74±4.56),inspiratory capacity: (62.19+18.74vs 64.44+18.66),Slow Vital Capacity (SVC) (63.96+19.90vs 66.19+25.50)without statistic significance.Conclusion: The EFIT shows no positive effects on pulmonary ventilation in CF patients with pulmonary exacerbation.