Die Inzisionen zur Entfernung des Primärtumors und für die

Granulocyte-macrophage colony stimulating factor (GM-CSF) gene expression and protein production were investigated in colorectal cancer cell lines and surgical specimens. In 6 of 6 established tumor lines, expression of the GM-CSF gene was observed by reverse transcription polymerase chain reaction (RT-PCR). Furthermore, for 2 of the lines, the cytokine was detectable in > or = 100 pg/ml amounts in culture supernatants by enzyme-linked immunosorbent assay tests. Addition of recombinant GM-CSF at doses ranging between 30 pg and 30 ng/ml did not appear to affect the proliferation of colorectal cancer cell lines as measured by 3H-thymidine incorporation. GM-CSF gene expression was then examined in surgical specimens by a densitometry-assisted, semiquantitative RT-PCR technique. In the 10 samples analyzed, significantly higher expression was detectable in tumors, as compared with autologous healthy mucosa sampled in the vicinity (2 cm) or at distance (10 cm) from the neoplastic focus. Immunohistochemistry studies performed on 13 specimens led to the identification of intracytoplasmic GM-CSF in tumor cells in 9 samples. In 6 of these, positivity of stromal fibroblasts and lymphocytes adjacent to the tumor was also observed. In contrast, intracellular GM-CSF was only detectable in 2 cases in untransformed epithelial cells, close to the neoplasm, but never in healthy mucosa at distance from the tumor. Infiltration by dendritic cells (DC) was also investigated. In 5 of 8 colorectal cancers tested, DC aggregates accounted for more than 10% of stromal cells. Lower numbers were detectable in healthy mucosa. However, DC infiltration could not be correlated with the presence of GM-CSF-positive neoplastic cells in the tumor specimens. Remarkably, cultured DC were unable to exert significant cytotoxic activity against colorectal cancer cells in vitro.

International Journal of CancerVolume 71, Issue 6 p. 1119-1121 Letter to the EditorFree Access High expression of MAGE-3 protein in squamous-cell lung carcinoma Claude Fischer, Claude Fischer Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorFred Gudat, Fred Gudat Department of Pathology, University of Basel, Basel, SwitzerlandSearch for more papers by this authorPeter Stulz, Peter Stulz Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorChristoph Noppen, Christoph Noppen Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorChristoph Schaefer, Christoph Schaefer Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorPaul Zajac, Paul Zajac Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorMarkus Trutmann, Markus Trutmann Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorThomas Kocher, Thomas Kocher Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorMarkus Zuber, Markus Zuber Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorFelix Harder, Felix Harder Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorMichael Heberer, Michael Heberer Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorGiulio C. Spagnoli, Corresponding Author Giulio C. Spagnoli Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSurgical Research Laboratory, Z.L.F., 20, Hebelstrasse, 4031, Basel, Switzerland. Fax:—41 61 265 3990.Search for more papers by this author Claude Fischer, Claude Fischer Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorFred Gudat, Fred Gudat Department of Pathology, University of Basel, Basel, SwitzerlandSearch for more papers by this authorPeter Stulz, Peter Stulz Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorChristoph Noppen, Christoph Noppen Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorChristoph Schaefer, Christoph Schaefer Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorPaul Zajac, Paul Zajac Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorMarkus Trutmann, Markus Trutmann Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorThomas Kocher, Thomas Kocher Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorMarkus Zuber, Markus Zuber Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorFelix Harder, Felix Harder Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorMichael Heberer, Michael Heberer Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSearch for more papers by this authorGiulio C. Spagnoli, Corresponding Author Giulio C. Spagnoli Departments of Surgery and Research, University of Basel, Basel, SwitzerlandSurgical Research Laboratory, Z.L.F., 20, Hebelstrasse, 4031, Basel, Switzerland. Fax:—41 61 265 3990.Search for more papers by this author First published: 06 December 1998 https://doi.org/10.1002/(SICI)1097-0215(19970611)71:6<1119::AID-IJC34>3.0.CO;2-5Citations: 26AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article.Citing Literature Volume71, Issue611 June 1997Pages 1119-1121 ReferencesRelatedInformation