Supplementary Figure 1 Legend from P-Cadherin Is Up-Regulated by the Antiestrogen ICI 182,780 and Promotes Invasion of Human Breast Cancer Cells

Upper left panel: Image representing a protein array of 507 proteins used for detection of soluble factors present in a mixture of CMCAAT from two patients. Each protein is spotted in duplicate. The boxes show the positive controls. The brackets indicate adiponectin (1) confirming the adipose nature of CMCAAT, GCSF (2), IL-6 (3), IL-8 (4), leptin (5), LIF (6), OSM (7). Upper right panels: western blot analysis of Y705 pSTAT3 of MCF-7 cells treated for 48h with control medium; neutralizing antibodies against hIL-6, hLIF and hIL-8 (1 µg/ml); rhIL-6, rhLIF and rhIL-8 (10 ng/ml); combination of the recombinant protein with the neutralizing antibody (same concentrations); western blot analysis of MCF-7 cells treated for 48h with CMCAAT and neutralizing antibodies against hIL-6, hLIF and hIL-8 at indicated concentrations. Lower panel: Phase-contrast images of MCF-7 cells treated or 48h with control medium, rhIL-6, rhLIF and rhIL-8 at indicated concentrations, CMCAAT and CMCAAT combined with neutralizing antibodies against hIL-6, hLIF and hIL-8 at indicated concentrations (Scale bar is 100 µm).

<p>F: forward primer, R: reverse primer.</p>

Supplementary Table S1: patient characteristics including age, sex, tumor type, and treatment

Supplementary Figure 1-7 on irradiation of CAF; effect of irradiated CAF on cancer cells; glutamine metabolism; proteome analysis of CM CAF; effect of recombinant IGF1 on cancer cells; effect of recombinant IGF1 on metabolism of cancer cells; schematic of in vivo set-up and Figure legends or respective figures

Supplementary Figure 2 from P-Cadherin Promotes Cell-Cell Adhesion and Counteracts Invasion in Human Melanoma

Graphs representing relative mRNA levels of OSMR, LIFR, GP130 and STAT3 in MCF-7 and SKBR3 cells treated for 48h with control medium or rhOSM (2 ng/ml).

Upper panels: Western blot analysis of E-cadherin, beta-catenin, vimentin, fibronectin, pancytokeratin and estrogen receptor alpha (ERα). Left panel: MCF-7 cells treated for 48h with control medium or CMCAAT. Right panel: MCF-7 cells stably transfected with GFP or OSM cDNA, or treated for 48h with rhOSM (1ng/ml). Tubulin serves as an internal control. Lower left panel: Confocal images of the F-actin cytoskeleton (red), nuclear staining DAPI (blue) and merged signals in MCF-7 cells treated for 48h with control medium or CMCAAT (scale bar is 20 μm) and confocal images of E-cadherin (green), beta-catenin (red) and merge signals with DAPI in MCF-7 cells treated for 48h with control medium or CMCAAT (scale bar is 10 μm). Lower right panel: Immunohistochemical staining of MCF-7-GFP and MCF-7-OSM tumor xenografts for E-cadherin, vimentin, pancytokeratin and ERα (scale bar is 25 µm).

<p>Western blot analysis of Y705 and S727 pSTAT3, STAT3, (p)Akt, pS6K, (p)Erk1/2 in MCF-7 cells treated for 48h with rhOSM (1ng/ml) and an Akt-inhibitor or Mek-inhibitor at the indicated concentrations.</p>

Supplementary Figure Legends 1-11, Methods from Implication of Metastasis Suppressor NM23-H1 in Maintaining Adherens Junctions and Limiting the Invasive Potential of Human Cancer Cells

Left panel: Graph representing proliferation rate of MCF-7-GFP and MCF-7-OSM cells in time (asterix indicates P = .068). Right upper panel: images of MCF-7-GFP and MCF-7-OSM aggregates embedded in type I collagen (scale bar is 100 µm). Right lower panel: Graph representing relative mRNA levels of OSMR, LIFR, GP130 and STAT3 in MCF-7-GFP and MCF-7-OSM cells.

<p>Supplemental materials and methods in addition to those mentioned in the manuscript.</p>

<p>Flow chart indicating CM^CAAT prepared from 25 breast cancer patients and tested in the experimental conditions described throughout the article. OSM concentration was measured by ELISA for the samples indicated in bold type (see also figure 3C).</p>

Supplementary Movie 2 from Implication of Metastasis Suppressor NM23-H1 in Maintaining Adherens Junctions and Limiting the Invasive Potential of Human Cancer Cells

<p>Flow chart indicating CM^CAAT prepared from 25 breast cancer patients and tested in the experimental conditions described throughout the article. OSM concentration was measured by ELISA for the samples indicated in bold type (see also figure 3C).</p>

<p>Western blot analysis of Y705 and S727 pSTAT3, STAT3, (p)Akt, pS6K, (p)Erk1/2 in MCF-7 cells treated for 48h with rhOSM (1ng/ml) and an Akt-inhibitor or Mek-inhibitor at the indicated concentrations.</p>

Supplementary Figure 2 from P-Cadherin Promotes Cell-Cell Adhesion and Counteracts Invasion in Human Melanoma

Supplementary Figure 1 from P-Cadherin Promotes Cell-Cell Adhesion and Counteracts Invasion in Human Melanoma

Peloruside A (1) is a novel macrolide with potent anti-cancer activity, discovered in 2000 from a marine sponge [1]. Like Paclitaxel (2) and Epothilon (3), Peloruside A is a microtubule-stabilizing agent, but it binds to a different binding site [2] and shows a better activity against multidrug-resistant cancer cell lines. Moreover, the presence of several hydroxyl groups causes a better solubility in the blood stream. The binding site at -tubuline and the biologically active conformation of (+)-Peloruside were confirmed in 2006 by Miller et al. [3a] via NMR and in 2014 by Steinmetz et al. [3b] via XR-analysis. The absolute configuration was established via total synthesis by De Brabander et al. [4]. Since then, a number of total syntheses have been reported [5].