The correct use of mitochondrial DNA (mtDNA) testing in the forensic context requires appropriate population databases to determine the relative rarity of the haplotype of the tested sample. The aim of this study was to evaluate the results of full HVRI and HVRII mtDNA sequences of 255 unrelated individuals and to compare the data to the previously published Czech and European population data The results indicate that the full control region sequencing can bring more precise population information that is useful for the comparison with other data sets and also for the forensic identification purposes.

142 unrelated males from the population sample of the Czech Republic were genotyped using the miniSTR pentaplexes I and II in 8 Y-chromosomal STRs not included in Y-filer kit (Applied Biosystems, USA). A total of 142 haplotypes were obtained of which 127 were unique. The most common haplotype were found in 4 samples. We observed the haplotype diversity of 0.998 and the discrimination capacity of 0.894.

The aim of this study was to develop an extraction protocol for bone samples based on ZR Genomic DNA Tissue MicroPrep kit and perform a quantitative comparison with the existing silica extraction protocol based on Qiagen columns and evaluate the effect of demineralization on the quantity of extracted DNA.

120 unrelated individuals from the population sample of the Czech Republic were genotyped using the AmpFℓSTR® NGM™ PCR amplification kit (Applied Biosystems). The population study was conducted to evaluate the usefulness of the five new STR loci (D10S1248, D22S1045, D2S441, D1S1656, and D12S391) included in the new European Standard Set and to establish the allele frequencies.