An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

The free-living nematode Caenorhabditis elegans is a well-characterized eukaryotic model organism. Recent glycomic analyses of the glycosylation potential of this worm revealed an extremely high structural variability of its N-glycans. Moreover, the glycan patterns of each developmental stage appeared to be unique. In this study we have determined the N-glycan profiles of wild-type embryos in comparison to mutant embryos arresting embryogenesis early before differentiation and causing extensive transformations of cell identities, which allows to follow the diversification of N-glycans during development using mass spectrometry. As a striking feature, wild-type embryos obtained from liquid culture expressed a less heterogeneous oligosaccharide pattern than embryos recovered from agar plates. N-glycan profiles of mutant embryos displayed, in part, distinct differences in comparison to wild-type embryos suggesting alterations in oligosaccharide trimming and processing, which may be linked to specific cell fate alterations in the embryos.

Die Erfindung betrifft Verbindungen, die insbesondere als Strukturmimetika prolinreicher Peptide eingesetzt werden konnen und demgemas in der Lage sind, PRM-Bindungsdomanen (proline-rich-motif binding domains) von Proteinen zu binden, die Erfindung betrifft weiterhin die Verwendung dieser Verbindungen als pharmazeutische Wirkstoffe sowie die Verwendung der pharmazeutischen Wirkstoffe zur Behandlung von bakteriellen Erkrankungen, neurodegenerativen Erkrankungen sowie Tumoren.

Das X markiert den Punkt, an dem die Aminosäure X (siehe Struktur; grau C, cyan H, blau N, rot O, gelb Doppelbindung) als Pro-Pro-Ersatz in zwei Peptide eingebaut werden konnte, die an die prolinreiche Motive erkennende Domäne Fyn-SH3 binden, ohne ihre Bindungsfähigkeit zu gefährden. Das Dipeptidanalogon X, das in einer Polyprolin-Typ-II-Helixkonformation fixiert ist, wurde durch stereoselektive Einführung einer Vinylidenbrücke in eine Diprolineinheit erzeugt. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.