Выполнено определение сакситоксина в пробах поверхностной воды, отобранной летом 2018 года в период массового цветения цианобактерий в прибрежной зоне заливов Ая, Тутай (западное побережье Байкала), Мухор (пролив Малое Море), Посольский сор и около села Турка (восточное побережье Байкала). Для определения сакситоксина использована методика определения, основанная на химической модификации сакситоксина 2,4-динитрофенилгидразином с последующей идентификацией гидразона сакситоксина методом жидкостной хроматографии с масс-спектрометрическим детектированием. Для проб с ожидаемой низкой концентрацией сакситоксина предложен усовершенствованный вариант методики. Показано, что содержание сакситоксина в воде во всех исследованных пробах ниже рекомендованной для питьевой воды (< 3 мкг/л).

Angewandte Chemie International EditionVolume 44, Issue 42 p. 6928-6931 Communication Lattice Expansion of Clathrate Hydrates of Methane Mixtures and Natural Gas† Satoshi Takeya Dr., Satoshi Takeya Dr. [email protected] Research Institute of Instrumentation Frontier (RIIF), National Institute of Advanced Industrial Science and Technology (AIST), Central 5, Higashi 1-1-1, Tsukuba 305-8565, Japan, Fax: (+81) 29-861-4506Search for more papers by this authorTsutomu Uchida Dr., Tsutomu Uchida Dr. Department of Applied Physics, Hokkaido University, N13W8 Sapporo 060-8628, JapanSearch for more papers by this authorYasushi Kamata Dr., Yasushi Kamata Dr. Methane Hydrate Research Laboratory, AIST, 2-17 Tukisamu Higashi Toyohira-ku, Sapporo 062-8517, JapanSearch for more papers by this authorJiro Nagao Dr., Jiro Nagao Dr. Methane Hydrate Research Laboratory, AIST, 2-17 Tukisamu Higashi Toyohira-ku, Sapporo 062-8517, JapanSearch for more papers by this authorMasato Kida, Masato Kida Kitami Institute of Technology, 165 Koencho, Kitami 090-8507, Japan, Fax: (+81) 157-26-9534Search for more papers by this authorHirotsugu Minami Dr., Hirotsugu Minami Dr. Kitami Institute of Technology, 165 Koencho, Kitami 090-8507, Japan, Fax: (+81) 157-26-9534Search for more papers by this authorHirotoshi Sakagami Dr., Hirotoshi Sakagami Dr. Kitami Institute of Technology, 165 Koencho, Kitami 090-8507, Japan, Fax: (+81) 157-26-9534Search for more papers by this authorAkihiro Hachikubo Dr., Akihiro Hachikubo Dr. Kitami Institute of Technology, 165 Koencho, Kitami 090-8507, Japan, Fax: (+81) 157-26-9534Search for more papers by this authorNobuo Takahashi Prof., Nobuo Takahashi Prof. Kitami Institute of Technology, 165 Koencho, Kitami 090-8507, Japan, Fax: (+81) 157-26-9534Search for more papers by this authorHitoshi Shoji Prof., Hitoshi Shoji Prof. [email protected] Kitami Institute of Technology, 165 Koencho, Kitami 090-8507, Japan, Fax: (+81) 157-26-9534Search for more papers by this authorOleg Khlystov Dr., Oleg Khlystov Dr. Russian Academy of Sciences Siberian Branch Limnological Institute, 3 Ulan-Batorskaya, Irkutsk 664033, RussiaSearch for more papers by this authorMikhail Grachev Dr., Mikhail Grachev Dr. Russian Academy of Sciences Siberian Branch Limnological Institute, 3 Ulan-Batorskaya, Irkutsk 664033, RussiaSearch for more papers by this authorValery Soloviev Dr., Valery Soloviev Dr. All-Russia Research Institute for Geology and Mineral Resources of the Ocean, Saint Petersburg 190121, RussiaSearch for more papers by this author Satoshi Takeya Dr., Satoshi Takeya Dr. [email protected] Research Institute of Instrumentation Frontier (RIIF), National Institute of Advanced Industrial Science and Technology (AIST), Central 5, Higashi 1-1-1, Tsukuba 305-8565, Japan, Fax: (+81) 29-861-4506Search for more papers by this authorTsutomu Uchida Dr., Tsutomu Uchida Dr. Department of Applied Physics, Hokkaido University, N13W8 Sapporo 060-8628, JapanSearch for more papers by this authorYasushi Kamata Dr., Yasushi Kamata Dr. Methane Hydrate Research Laboratory, AIST, 2-17 Tukisamu Higashi Toyohira-ku, Sapporo 062-8517, JapanSearch for more papers by this authorJiro Nagao Dr., Jiro Nagao Dr. Methane Hydrate Research Laboratory, AIST, 2-17 Tukisamu Higashi Toyohira-ku, Sapporo 062-8517, JapanSearch for more papers by this authorMasato Kida, Masato Kida Kitami Institute of Technology, 165 Koencho, Kitami 090-8507, Japan, Fax: (+81) 157-26-9534Search for more papers by this authorHirotsugu Minami Dr., Hirotsugu Minami Dr. Kitami Institute of Technology, 165 Koencho, Kitami 090-8507, Japan, Fax: (+81) 157-26-9534Search for more papers by this authorHirotoshi Sakagami Dr., Hirotoshi Sakagami Dr. Kitami Institute of Technology, 165 Koencho, Kitami 090-8507, Japan, Fax: (+81) 157-26-9534Search for more papers by this authorAkihiro Hachikubo Dr., Akihiro Hachikubo Dr. Kitami Institute of Technology, 165 Koencho, Kitami 090-8507, Japan, Fax: (+81) 157-26-9534Search for more papers by this authorNobuo Takahashi Prof., Nobuo Takahashi Prof. Kitami Institute of Technology, 165 Koencho, Kitami 090-8507, Japan, Fax: (+81) 157-26-9534Search for more papers by this authorHitoshi Shoji Prof., Hitoshi Shoji Prof. [email protected] Kitami Institute of Technology, 165 Koencho, Kitami 090-8507, Japan, Fax: (+81) 157-26-9534Search for more papers by this authorOleg Khlystov Dr., Oleg Khlystov Dr. Russian Academy of Sciences Siberian Branch Limnological Institute, 3 Ulan-Batorskaya, Irkutsk 664033, RussiaSearch for more papers by this authorMikhail Grachev Dr., Mikhail Grachev Dr. Russian Academy of Sciences Siberian Branch Limnological Institute, 3 Ulan-Batorskaya, Irkutsk 664033, RussiaSearch for more papers by this authorValery Soloviev Dr., Valery Soloviev Dr. All-Russia Research Institute for Geology and Mineral Resources of the Ocean, Saint Petersburg 190121, RussiaSearch for more papers by this author First published: 25 October 2005 https://doi.org/10.1002/anie.200501845Citations: 33 † S.T. thanks H. Fujihisa, Y. Gotoh, and K. Honda of AIST for their valuable comments and discussions, and H. Narita of AIST for his support in experiments. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Graphical Abstract Structural transition from type I to type II is accompanied by a large increase in lattice constant a in CH4+C2H6 clathrate hydrates (see picture). In contrast, the lattice constants of CH4+CO2 clathrate hydrates are independent of composition. Data for a natural gas hydrate from the sediments of Lake Baikal are also consistent with these trends. References 1 1aH. R. Müller, M. von Stackelberg, Naturwissenschaften 1952, 39, 20; 1bM. von Stackelberg, H. R. Müller, Naturwissenschaften 1951, 38, 456; 1cJ. A. Ripmeester, J. S. Tse, C. I. Ratcliffe, B. M. Powell, Nature 1987, 325, 135. 2 2aK. A. Udachin, J. A. Ripmeester, Nature 1999, 397, 420; 2bK. A. Udachin, C. I. Ratcliffe, J. A. Ripmeester, Angew. Chem. 2001, 113, 1343; Angew. Chem. Int. Ed. 2001, 40, 1303. 3E. D. Sloan, Jr., Clathrate Hydrate of Natural Gases, 2nd ed., Marcel Dekker, New York, 1998. 4 4aS. Subramanian, R. A. Kini, S. F. Dec, E. D. Sloan, Jr., Chem. Eng. Sci. 2000, 55, 1981; 4bS. Subramanian, A. L. Ballard, R. A. Kini, S. F. Dec, E. D. Sloan, Jr., Chem. Eng. Sci. 2000, 55, 5763; 4cS. Takeya, Y. Kamata, T. Uchida, J. Nagao, T. Ebinuma, H. Narita, Can. J. Phys. 2003, 81, 479. 5T. Uchida, S. Takeya, Y. Kamata, I. Y. Ikeda, J. Nagao, T. Ebinuma, H. Narita, O. Zatsepina, B. A. Buffett, J. Phys. Chem. B 2002, 106, 12426. 6 6aD. K. Staykova, W. F. Kuhs, A. N. Salamatin, T. Hansen, J. Phys. Chem. B 2003, 107, 10299; 6bJ. M. Schicks, J. A. Ripmeester, Angew. Chem. 2004, 116, 3372; Angew. Chem. Int. Ed. 2004, 43, 3310. 7E. D. Sloan, Jr., Nature 2003, 426, 353. 8 8aYu.-T. Seo, H. Lee, J. Phys. Chem. B 2001, 105, 10084; 8bH. Lee, Y. Seo, Yu.-T. Seo, I. L. Moudrakovski, J. A. Ripmeester, Angew. Chem. 2003, 115, 5202; Angew. Chem. Int. Ed. 2003, 42, 5048. 9 9aL. A. Ballard, E. D. Sloan, Jr., Chem. Eng. Sci. 2000, 55, 5773; 9bY. Koyama, H. Tanaka, K. Koga, J. Chem. Phys. 2005, 122, 074503. 10K. A. Udachin, C. I. Ratcliffe, J. A. Ripmeester, J. Phys. Chem. B 2001, 105, 4200. 11C. Gutt, B. Asmussen, W. Press, M. R. Johnson, Y. P. Handa, J. S. Tse, J. Chem. Phys. 2000, 113, 4713. 12C. J. Rawn, A. J. Rondinone, B. C. Chakoumakos, S. L. Marshall, L. A. Stern, S. Circone, S. H. Kirby, C. Y. Jones, B. H. Toby, Y. Ishii, Proc. 4th Int. Conf. Gas Hydrate, Yokohama, 2002, p. 595. 13H. Hirai, T. Kondo, M. Hasegawa, T. Yagi, Y. Yamamoto, T. Komai, K. Nagashima, M. Sakashita., H. Fujihisa, K. Aoki, J. Phys. Chem. B 2000, 104, 1429. 14K. A. Udachin, C. I. Ratcliffe, J. A. Ripmeester, J. Supramol. Chem. 2002, 2, 405. 15H. M. Rietveld, J. Appl. Crystallogr. 1969, 2, 65. 16Z. Huo, K. Hester, D. Sloan, Jr., AIChE J. 2003, 49, 1300. 17J. Klerkx, T. I. Zemskaya, T. V. Matveeva, O. M. Khlystov, B. B. Namsaraev, O. P. Dagurova, L. P. Golobokova, S. S. Vorob'eva, T. P. Pogodaeva, N. G. Granin, G. V. Kalmychkov, V. A. Ponomarchuk, H. Shoji, L. L. Mazurenko, V. V. Kaulio, V. A. Solov'ev, M. A. Grachev, Dokl. Earth Sci. 2003, 393A, 1342. 18F. Izumi, T. Ikeda, Mater. Sci. Forum 2000, 321–324, 198. 19K. Röttger, A. Endriss, J. Ihringer, S. Doyle, W. F. Kuhs, Acta Crystallogr. Sect. B 1994, 50, 644. Citing Literature Volume44, Issue42October 28, 2005Pages 6928-6931 ReferencesRelatedInformation

Yeast valine tRNA1 was chemically modified with chlorambucil N-hydroxysuccinimide ester. tthe reagent was attached covalently to the valine residue of valyl-tRNA1Val under the conditions which prevented tRNA from alkylation. Chlorambucilyl-valyl-tRNA1Val thus obtained was separated from excess reagent and incubated in an aqueous solution at neutral pH in the presence of Mg++ions. Highly efficient intramolecular self-alkylation of chlorambucilyl-valyl-tRNA1Val took place. The chlorambucil residue bound covalently to the amino group of the valine residue of tRNA1Val alkylates the 5'-terminal phosphate group of the molecule, and its 3'-terminal sequence -A-C-C-A.