Objective The aim of this study was to establish a risk scoring system for the decreased glomerular filtration rate (GFR) in Chinese general population. Methods Totally 781 participants who underwent a health checkup in The First Affiliated Hospital of Nanjing Medical University from January to September 2017 were involved in the study. Significant variables chosen by multivariable logistic regression analysis were allocated the integral scores in proportion to its odds ratio (OR), and then the risk of decreased GFR was assessed based on the scores. Results The people with abnormal homocysteine (Hcy) level (OR: 1.534, 95% confidential interval [CI]: 1.075-2.190, P = .018), males (OR: 2.054, 95%CI: 1.365-3.092, P < .001), and those at the age of 46-52 years (OR: 2.943, 95%CI: 1.546-5.605, P = .001), 52-59 years (OR: 3.664, 95%CI: 1.937-6.931, P < .001) and >= 59 years (OR: 13.452, 95%CI: 7.339-24.657, P < .001) were subjected to GFR reduction. These three variables were allocated the integral scores in proportion to its OR, and four risk categories were divided according to the scores. The prevalence of the decreased GFR in people with low (score 0-4, n = 8), below the average (score 4-6, n = 37), above the average (score 6-13, n = 47), and high risks (score >= 13, n = 103) was 5.26%, 16.89%, 22.93% and 50.24%, respectively, and this prevalence raised with the increase of scores (P < .001). Conclusions A risk scoring system is developed in this study, which may offer a specific risk stratification for GFR reduction in Chinese general population.

End-stage renal disease (ESRD) was defined as start of renal replacement therapy or death due to kidney disease. However, death due to acute kidney injury was not included. It typically occurs when chronic renal failure progresses to a point where the kidneys are permanently functioning at less than 10% of their capacity. Oxidative stress (OS) plays a crucial role in ESRD. Nicotinamide adenine dinucleotide phosphate (NADPH) is one of the most important enzymes during oxidative stress. Cytochrome b light chain (CYBA), encoded by a polymorphic gene, which is a critical component of the nicotinamide adenine dinucleotide (NADH)/NADPH oxidase system and plays an important role in electron transport and superoxide anion production, is located on chromosome band 16q24 and has six exons spanning almost 7.76 kb of genomic DNA. CYBA gene polymorphisms can influence the activity of NADPH oxidase. To evaluate the association between CYBA gene polymorphisms and ESRD, we genotyped five CYBA polymorphisms using TaqMan allelic discrimination assay on DNA samples from 306 healthy controls and 332 patients with ESRD. Our results suggested that rs1049255 polymorphism of CYBA modified the risk of ESRD (p = 0.019; OR = 0.625; 95% CI = 0.424-0.921). GG genotype and G allele might be a protective factor against the risk of ESRD, especially in patients with chronic glomerulonephritis.

Background and Purpose: Recent studies reported that peripheral blood CD34+ cells and endothelial progenitor cells contributed to angiogenesis in patients with moyamoya disease (MMD), and stromal cell‐derived factor‐1α (SDF‐1α) modulated angiogenesis by interacting with corresponding CXCR4 receptors. We investigated in this study whether SDF‐1α/CXCR4 axis involved in neovascularization in patients with MMD.Methods: Eighteen patients with MMD and twelve healthy individuals were enrolled. Patients with MMD detected by cerebral angiography were retrieved from the Nanjing Stroke Registry Program. Peripheral whole blood cells were double stained with anti‐CD34 and anti‐CXCR4 (CD184). Numbers of CD34+ CXCR4+ cells were analyzed by flow cytometry. Plasma concentration of SDF‐1α was determined by enzyme‐linked immunosorbent assay.Results: In consecutive patients with MMD, the number of CD34+ CXCR4+ cells was greater than healthy individuals (54.1 ± 28.6/μl peripheral blood (PB) versus 24.6 ± 25.6/μl PB; P = 0.015). Plasma SDF‐1α level was significantly higher in patients with MMD compared with healthy controls (1676.76 ± 198.65 pg/ml versus 1508.26 ± 137.27 pg/ml; P = 0.016). No significant correlation between number of CD34+ CXCR4+ cells and plasma SDF‐1α level in the patients with MMD was observed (r = 0.185; P = 0.46).Conclusion: This study indicated that increased levels of circulating SDF‐1α and CD34+ CXCR4+ cells in patients with MMD, which may play an important role in the vasculogenesis in MMD.