AbstractThermolysis of the strained furoxans (Ia) to (Ic) in toluene saturated with SO2 yields the 1,3‐di‐isocyanato‐cyclopentanes (IVa) to (IVc).

The preparation, crystallization and low-resolution structure determination of beta-lactamase (EC 3.5.2.6, ‘penicillinase’) from Staphylococcus aureus is described. The enzyme crystallizes in space group I222 with 1 molecule per asymmetric unit and cell dimensions a = 5.45(1), b = 9.39(1) and c = 13.87(2) nm. The structure was determined at 0.5 nm resolution by using phases calculated from (NH4)2Pt(CN)4 and KAu(CN)2 derivatives. The mean figure of merit mean value of m, for the 1106 reflexions used was 0.70. Difference Fourier syntheses for data collected from crystals soaked in platinum D-methionine and in 6-(4-hydroxy-3,5-di-iodobenzamido)penicilloic acid revealed the likely position of the active site of the enzyme.

AbstractBeim Erhitzen in Xylol werden die Oxathiazole (I) zu den Nitrilsulfiden (II) decarboxyliert, die in situ mit den Carbonylverbindungen (III) 1,3‐dipolare Cycloaddition zu den 2,2,5‐trisubstituierten Oxathiazolen (IV) eingehen; neben (IVe) bzw. (IVf) entstehen in 65 bzw. 59 proz. Ausb. p‐Chlor‐ bzw. p‐Methoxy‐benzonitril.

shO\JD in the Figure.The overall dimensions of the molecule are approximately 33 X 39 X 34 A. The heme-heme distances and angles are listed in the Table.