房建工程质量安全事关国计民生的大事，近年来在国家相关部门的大力重视下，因房建工程质量问题而产生的相关事故、纠纷的现象呈现出明显的下降趋势，但是土建工程质量通病问题仍然存在。下面本文就此问题进行重点阐述。

An increased level of spontaneous DNA double-strand breaks in Pten deficient cells was previously reported. Here we investigated whether and how the anti-oncogene pten may regulate the expression of rad51,a key component for homologous recombination in DNA double-strand break repairs.The rad51 mRNA level in the Pten -/- MEFs cells was down-regulated as shown by real-time PCR. After LY294002 (an antagonist of PTEN to inhibit PI3K) treatment,the RAD51 mRNA was increased. The transfection of Flag-Akt WT (Akt wild-type as the PI3K down-stream target) or Flag-AC (constitutively activated Akt) decreased the RAD51 at both mRNA and protein levels in Pten +/+ MEFs cells. In contrast,the Pten WT or Akt-DN (inactivated Akt) transfection augmented levels of rad51 expression as compared to the cells transfected with the control Flag-Vec vector. The siRNA-mediated suppression of akt resulted in an increased expression of RAD51 protein in pten -/- MEFs cells. The results suggested that PTEN positively regulated rad51 expression,and the PI3K /Akt signaling pathway might be involved.

The paper provided an idea of traffic presure information analysis system based on GIS.It Analyzed and discussed the design of this system and processing of the functional modules and data base.

Mulberry leaves soaked in fluoride solution were used to feed fluoride-resistant and susceptible strains of silkworm to investigate the effect of fluoride on their acetylcholinesterase(AChE)activity.The experimental results showed that compared with the control,fluoride soaking decreased AChE activity of both silkworm strains,the extent of decrease being smaller in the resistant strain than in the susceptible strain.The protein content of zymogen of the larvae treated with fluoride was higher than control group in the fluoride-resistant strain and lower than control group in the fluoride-susceptible strain.These results show that in the resistant strain the AChE gene expression increased to make up for the influence of the AChE activity and helped to maintain a similar AChE activity level,so that the silkworm produces a fluoride resistance.

Objective To investigate the effect of Pten gene knock-out on expression of Rad51 gene and the potential mecha-nism of the effect.Methods The mRNA transcription levels of Rad51 gene related to repair by homologous recombination in Pten~(+/+)MEFs and Pten~(-/-)MEFs,the radiosensitivities of the two kinds of MEFs,as well as Rad51 mRNA transcription levels in the two kinds of MEFs after radiation,were compared by semi-quantitative RT-PeR and clone formation assay,and the potential mechanism was in-vesfigated.Results The Rad51 mRNA transcription level in Pten~(-/-)MEFs was significantly lower than that in Pten~(+/+)MEFs.Com-pared with that of Pten~(+/+)MEFs.the mdiosensitivity of Pten~(-/-)MEFs also decreased significantly.However,both the Rad51 mRNAtranscription levels in Pten~(-/-)MEFs after radiation and after treatment with LY-294002,an inhibitor of PI 3K/AKT signal pathway,in-creased significantly.Conclusion Abnormal expression of Rad51 gene WaS observed after knock-out of Pten gene.Pten might regu-late the expression of Rad51 gene through P13K/AKT signal pathway.

Shaoyang Huagu play is one of the most characteristic drama.The formation,variety,drama catalogue,music and dance performances of Shaoyang Huagu drama and the distinction contact between Hunan Huagu plays are discussed.Based on the art characteristics research,by studying the art performance,historical formation and present status of Shaoyang Huagu play,the future of Hunan local drama is promoted continuously.

Development of the evaluation of investment projects theory is a process that gradually adapted to the requirements of economic activity.Commonly it used investment decision-making theories: net present value;real options and options of the game law.It needs to sum up clearly the evolution of the evaluation of investment projects theory in order to rationalize the large number of theoretical methods,as well as deep understanding of its development and orientation.The traditional method of quantitative evaluation on the investment projects provides a quantitative basis for investment decisions.The value of investing projects on option games was evaluated based on the theory of real option and game approach.It brings psychology and sociology into consideration led the Project value to be more practical.

介绍了利用沥青再生技术对G105路面改造的指导思想和方法,阐述了该技术的优点,分析了目前存在的问题,提出针对我国实际情况,进行科学系统的检测和统计分析,加大我国的再生沥青混合料的设计方法研究的观点。

Taking a bridge project,the application of SFRC to the pavement of bridge deck is discoursed on,including the mechanism,designing principle and steps of mix proportion of SFRC and construction process;construction requirements are presented.