In this paper, we present virtualization of the pressure control demonstration unit. The two main objectives were: to determine a dynamic mathematical model of the unit, and to use the mathematical model to virtualize the process that enables open and closed loop simulations. The dynamic model of the unit, which was developed based on experimental input-output data, shows Fit to Working Data greater than 95 %. Finally, the unit was virtualized in a form of a graphical user interface that hides all the modeling components from the user. The virtual unit is thus designed to enable students with limited or no prior knowledge of control theory and modeling of dynamic systems to study and analyze the dynamics of the system and to observe the effects of feedback control mechanisms.

The process of hematopoiesis is subject to substantial ontogenic remodeling that is accompanied by alterations in cellular fate during both development and disease. We combine state-of-the-art mass spectrometry with extensive functional assays to gain insight into ontogeny-specific proteomic mechanisms regulating hematopoiesis. Through deep coverage of the cellular proteome of fetal and adult lympho-myeloid multipotent progenitors (LMPPs), common lymphoid progenitors (CLPs), and granulocyte-monocyte progenitors (GMPs), we establish that features traditionally attributed to adult hematopoiesis are conserved across lymphoid and myeloid lineages, whereas generic fetal features are suppressed in GMPs. We reveal molecular and functional evidence for a diminished granulocyte differentiation capacity in fetal LMPPs and GMPs relative to their adult counterparts. Our data indicate an ontogeny-specific requirement of myosin activity for myelopoiesis in LMPPs. Finally, we uncover an ontogenic shift in the monocytic differentiation capacity of GMPs, partially driven by a differential expression of Irf8 during fetal and adult life.