This study was designed to evaluate the anti-inflammatory effect of recombinant human kallistatin (Kal) on ulcerative colitis (UC) in the mouse model. Acute colitis was induced by administration of 4% dextran sodium suffate (DSS) to KM mice for 7 days. The mice were then randomized into 5 groups: model control, Kal 0.2 mg·kg(-1)·d(-1), 1.0 mg·kg(-1)·d(-1) and 2.0 mg·kg-1·d(-1) group, salazosulfapyridine (SASP) group. Ten age-matched normal KM mouse were administered with saline in the normal control. The weight, colon length, inflammation factor (MPO/SOD/MDA) and TNF-α/IL-10 levels among the five groups of mice were determined. The results showed that histological index score and MPO/MDA/TNF-α levels of high-dose Kal treatment group and SASP group were significantly lower compared with the model group (P < 0.01), but the weight, colon length, IL-10 level and SOD activity were significant higher than the model group (P < 0.01), approaching the normal group. These parameters showed that Kal can significantly relieve the UC state in a dose-dependent manner. This study demonstrates that Kal significantly remits UC in mice, and participates in the regulation of inflammatory cytokines TNF-α/IL-10 levels and has some antioxidant activity.

Ulcerative colitis (UC) is a kind of the intestinal immune system disorder disease, the exact pathogen esis of UC remains undetermined. Current therapeutic strategie s for treating UC mainly relied on nonspecific immune-suppressive therapies. Thus, new therapies a re highlight to develop. We have investigated the e ff cts of a kallistatin (Kal)-encoding plasmid in mice acute ul cerative colitis (AUC) by intramuscular electropora tion. The model of mice AUC was induced by giving 4% dextran sulfate sodium (DSS) solutions as drinking water 6 days, which was evidenced by Disease activity index (DAI) estimation. Plasmid DNAs were electrotransfered in to skeletal muscle, with the best electroporation conditions. O ur results suggested that Kal was sufficiently expr ssed after injection of naked plasmid by intramuscular electro poration. In the Kal gene-transferred group, the co lonic myeloperoxidase (MPO) activity, malondialdehyde lev el and TNFα content were declined , the superoxide dismutase(SOD)activity was enhanced, and all had si gnificant differences (p <0.05) compared to those in non-Kal-treated group. The electrotransfed Kal plas mid can express transgene effectively in vivo and t his might be a promising approach for treating UC.

This study is to investigate whether naked plasmid DNA can effectively transfect lung cancer related cells and express human kallistatin, an endogenous protein that inhibits angiogenesis and tumor growth, and to explore the biological activity of the low-level expressed kallistatin to lung cancer in vitro and in vivo. The plasmids were delivered with Lipofectamine 2000 to transfect various lung cancer related cells. Kal expression was determined by ELISA. The biological effects of Kal expression on proliferation, migration and apoptosis rate of the cells were examined. In subcutaneous NCI-H446 xenograft model, pKal was injected directly into tumors, the changes of CD34, Ki-67 and E-cadherin expression were detected with immunohistochemical assay, the tumor apoptosis was analyzed with TUNEL assay. Both the endothelial cell and lung cancer cells could express kallistatin after plasmid transfection. The proliferation and migration of human umbilical vein endothelial cells were inhibited, but the apoptosis rate was not affected. The proliferation rates of all the three tested lung cancer cells, such as NCI-H446, NCI-H460 and A549, were inhibited, and their apoptosis rates were enhanced, but different cells behaved differently. In subcutaneous NCI-H446 xenograft model, intratumor injection of pKal inhibited the growth of lung cancer by reducing angiogenesis and proliferation of tumor cells. In conclusion, this study demonstrated the efficacy of plasmid-mediated expression of kallistatin to lung cancer related cells, thus providing a basis for their clinical application in the treatment of lung cancer.

Objective:To investigate the anti-diabetic effect of Danzhi Jiangtang capsule(DJC) on experimental diabetic rats.Method:Experimental diabetic model in rats was induced by the injection of streptozocin.The models were divided randomly into 6 groups:model group(normal saline ig),phenformin hydrochloride group(75 mg·kg-1,ig),Tangmaikang group(1.5 g·kg-1,ig) and DJC low,middle,high dose(13,6.5,3.2 g·kg-1,ig) groups(n =8 each).Rats were selected as normal group(nomal saline ig).After 20 days of administration,effects of body weight,blood glucose,glucose tolerance and insulin level of rats were observed.Result:The DJC high dose group could significantly decrease level of fasting blood glucose(P < 0.01) and improve glucose tolerance(P < 0.01).All the DJC groups could significantly increase body weight of diabetic rats(P < 0.01,P < 0.05) and decrease level of glycosylated serum protein(P < 0.01).No obvious effect of DJC on insulin level.Conclusion:DJC has significant anti-diabetic effect on experimental diabetic rats.

OBJECTIVE To review the progress of studies on protective mechanism of Radix Astragali in vascular endothelial cells. METHODS The summarization of the progress of studies on protective mechanism of tonifying qi chinese medicine Radix Astragali as well as its effective components in vascular endothelial cells were based on referring to the related literature in recent years.RESULTS The protective function of Radix Astragali as well as its effective components in vascular endothelial cells in means of promoting endothelial cells proliferation,inhibiting cells apoptosis,promoting angiogenesis,boosting nitric oxide composition,intervening cells adhesion,ameliorating vasodilatation function,regulating endothelial active substance secretion,antioxidation and so on,revealed significance therapeutic value in cardiovascular diseases and other diseases with endothelum disfunction. CONCLUSION Because of the multiple-targeted protective effect of Radix Astragali in vascular endothelial cells,the summarization of the protective mechanism and character of Radix Astragali in vascular endothelial cells had possessed of vital theoretical value with regard to exploring"tonifying qi"effective of Radix Astragali in prevention and treatment of blood stasis.