The aim of this research is to evaluate the effect of tetramethylpyrazine (TMP) and connective tissue growth factor (CTGF) miRNA plasmids on the expressive levels of CTGF, transforming growth factor-beta (TGFbeta) and type I collagen of rat hepatic stellate cells (HSC) which are stimulated by high glucose. The rat HSCs which were successfully transfected rat CTGF miRNA plasmids and the rat HSCs which were successfully transfected negative plasmids were cultured in vitro. After stimulus of the TMP and the high glucose, the protein levels and gene expressive levels of CTGF, TGF-beta and type I collagen were tested. The results indicated that high glucose increased the expression of CTGF mRNA, CTGF protein, TGF-beta mRNA,TGF-beta protein and type I collagen (P < 0.05). The expressive levels of CTGF mRNA, CTGF protein, TGF-beta mRNA, TGF-beta and type I collagen in TMP group were lower than those in high glucose group and showed statistically significant differences (P < 0.05). Compared with high glucose group, the expressive levels of CTGF mRNA, CTGF protein, TGF-beta mRNA, TGF-beta and type I collagen in rat CTGF miRNA plasmid interference group were significantly lower (P < 0.05). However, no statistically significant difference was found in CTGF mRNA and CTGF protein levels between TMP group and CTGF miRNA group (P > 0.05), while type I collagen levels showed statistically significant differences (P < 0.05). It is concluded that high glucose could promote the expressions of CTGF, TGF-beta and type I collagen, and TMP and rat CTGF miRNA plasmids could reduce the expressions of CTGF, TGF-beta, type I collagen.

BACKGROUND:Liver transplantation is still facing many problems,such as lack of donor livers,immune rejection after transplantation and its high costs,thus making it restricted in clinic application.OBJECTIVE:To describe autologous bone marrow stem cell transplantation in the clinical stage as one of the most promising technology that autologous bone marrow stem cells can differentiate into mature liver cells to replace the damaged liver functioning,which can improve symptoms and quality of life in patients.METHODS:A computer-based online search of PubMed database,CNKI,Wanfang database and VIP database was performed for articles regarding autologous bone marrow stem cells to treat hepatic cirrhosis,with the key words of "bone marrow stem cell,autologous bone marrow stem cell transplantation,hepatic cirrhosis" in Chinese and "bone marrow stem cell,autologous bone marrow stem cell,hepatic cirrhosis" in English.Relevant articles published in recent years or in authorized journals were preferred.Repetitive studies were excluded.Finally,20 articles were included.RESULTS AND CONCLUSION:Basic and clinical research about autologous bone marrow stem cell transplantation indicate that autologous bone marrow stem cell transplantation has a broad prospect in the treatment of hepatic cirrhosis.