Background: Diabetes mellitus is a global health challenge associated with chronic complications like diabetic nephropathy and diabetic foot infections. Diabetic nephropathy, mediated by hyperglycemia-induced activation of the polyol pathway, represents a primary cause of end-stage renal disease. Additionally, infections caused by multidrug-resistant bacteria like Enterococcus faecalis lead to amputations and contribute to morbidity in diabetic patients. Methods: In this study, we synthetized nitrogen-doped carbon dots (N-CDs) using succinic acid with either hexamethylenediamine (N-HCD) or ethylenediamine (N-ECD) and evaluated their potential therapeutic applications. Results: Both N-HCD and N-ECD demonstrated a significant reduction in aldose reductase (AR) and sorbitol dehydrogenase (SDH) in vitro, with a substantial reduction in polyol pathway enzymatic activity. Furthermore, these N-CDs exhibited antibacterial activity against E. faecalis in vitro. Conclusions: Taken together, our findings suggest that N-HCD and N-ECD represent promising candidates for addressing diabetes-related complications and warrant further investigation for potential drug delivery applications.

Ephedra fragilis is a medicinal plant known for its richness in bioactive compounds and therapeutic potential. This study aimed to evaluate and compare the antioxidant activity and phytochemical content of aqueous extracts obtained by decoction and infusion. Various classes of secondary metabolites, including polyphenols, flavonoids, flavonols, tannins, and hydroxycinnamic acids, were quantified. Antioxidant activity was assessed using multiple complementary assays. The results showed that the decoction method yielded extracts with notably higher antioxidant capacity compared to the infusion. High-performance liquid chromatography analysis confirmed the presence of several major compounds, such as caffeic acid, catechin, and rutin. These findings demonstrate that decoction is a more efficient extraction method for recovering antioxidant constituents from Ephedra fragilis, supporting its potential use in health-related applications and functional products.

This study investigated the acute oral toxicity and in vitro cytotoxicity of Ephedra fragilis decoction extract to provide a preliminary evaluation of its safety profile in the context of traditional medicinal use. Acute toxicity was assessed in Sprague-Dawley rats in accordance with OECD Guideline 425 using single oral doses of 500, 2000, and 4000 mg/kg. Animals were monitored for 14 days for clinical signs, mortality, and general behavior, followed by biochemical and histopathological analyses of liver and kidney tissues. In parallel, in vitro cytotoxicity was evaluated in peripheral blood mononuclear cells (PBMCs) using the MTT assay at concentrations up to 1200 & micro;g/mL. No mortality or overt clinical signs of toxicity were observed at any tested dose, including 4000 mg/kg. Histopathological analysis revealed no major structural alterations in hepatic or renal tissues. However, dose-dependent variations were observed in selected biochemical parameters, including decreased serum urea levels at higher doses, while creatinine levels remained within physiological ranges. These findings may suggest metabolic modulation rather than clear renal impairment, although further mechanistic studies are required. The MTT assay indicated high cell viability across all tested concentrations, suggesting low in vitro cytotoxicity. Overall, the extract exhibited a low level of acute oral toxicity, with an estimated LD50 greater than 4000 mg/kg. Nevertheless, the biochemical changes observed at higher doses indicate that further sub-chronic and chronic toxicity studies are necessary to fully establish its safety profile.

The emergence of multidrug-resistant Enterobacteriaceae represents a major threat to global public health. Among the key resistance mechanisms, the production of β-lactamases enzymes that hydrolyze the β-lactam ring plays a central role in compromising antibiotic efficacy. Targeting these enzymes, particularly carbapenemases, remains a promising strategy for the development of novel antibacterial agents. In this study, we focused on the carbapenemase GES-5 and performed a large-scale virtual screening of 7527 natural compounds derived from two independent databases (1012 and 6515 compounds, respectively). Molecular docking was carried out using AutoDock Vina to identify high-affinity inhibitors. The pharmacokinetic and toxicity profiles of the top-ranked hits were evaluated through ADMET predictions using SwissADME and pkCSM. To further assess binding stability and dynamic behavior, 100 ns molecular dynamics simulations were conducted using GROMACS. Docking results revealed several compounds exhibiting low binding affinities (≤ -10 kcal/mol) toward the GES-5 active site. Among these, Riccardin C, Daturametelin A, and 2-hydroxysenaganolide emerged as the most promising candidates based on their favorable interaction patterns. ADMET analysis indicated that the majority of selected compounds possess acceptable pharmacokinetic properties and low predicted toxicity. Molecular dynamics simulations confirmed the structural stability of the protein-ligand complexes throughout the 100 ns trajectory. Overall, these findings identify promising natural inhibitors of GES-5 and provide a robust in silico framework for further experimental validation and lead optimization.

Lavandula mairei (L. mairei) is a species of plant from the Lamiaceae family that is endemic to Morocco and known for its aromatic and medicinal properties. The aim of the study was to evaluate toxicity and antidiabetic properties of L. mairei essential oil (EO). The toxicity study was conducted on mice by administration of a single oral dose, followed by observation for 14 days. Clinical signs and symptoms associated with each dose administered were closely monitored. Serum biochemical analyses of various biomarkers of hepatic and renal functions, lipid profile, as well as histological sections were examined. Moreover, the antidiabetic activity was evaluated in vivo in type 1 diabetic rats and, in vitro assays were carried out using enzyme suspensions prepared from the liver of type 2 diabetic rats. Additionally, we assessed the ability of the EO to inhibit the enzymatic activity of polyol pathway, α -amylase and α -glucosidase. The biochemical results indicated that the EO was unsafe at a dose of 1000mg/kg body weight, while histological sections showed clear signs of hepatotoxicity and nephrotoxicity at both 1000mg/kg and 500mg/kg. The EO showed a significant antidiabetic potential with a decrease in glycaemia and significant inhibitory effects on some metabolic enzymes. L. mairei EO may be a promising natural remedy for the management of diabetes, but rigorous scientific studies are needed to validate its efficacy and safety.

Type 2 Diabetes Mellitus (T2DM) is a chronic metabolic disorder characterized by insulin resistance and impaired glucose metabolism. Glimepiride, a sulfonylurea drug, is commonly used to manage blood glucose levels in T2DM patients. However, the efficacy of various active pharmaceutical ingredients (APIs) of Glimepiride has not been thoroughly compared. Here, we systematically evaluate nine Glimepiride APIs for their modulation of oxidative stress pathways via computational and laboratory models. Molecular docking simulations were performed to investigate the binding affinity between Glimepiride and aldose reductase (AR), revealing a strong binding interaction. Furthermore, High-Performance Liquid Chromatography analysis was conducted to assess the purity of the APIs, showing consistent results with all samples exceeding 99.5 Molecular docking studies have shown a strong binding affinity between glimepiride and aldose reductase. In vitro assays revealed that Glimepiride Active Pharmaceutical Ingredients exhibited a global therapeutic effect by inhibiting aldose reductase, activating glutathione reductase and significantly reducing MDA levels. Differences in therapeutic efficacy between Active Pharmaceutical Ingredients are related to bioavailability, formulation and pharmacokinetics.

The red alga Chondracanthus acicularis, a carrageenophyte species, represents a valuable but underexplored marine resource with significant biochemical potential. This study examined the seasonal variability of its primary and secondary metabolites at two Moroccan coastal sites: El Jadida and Sidi Rahal. Key metabolites, including pigments, polysaccharides, proteins, lipids, polyphenols, and flavonoids, were analyzed. Polysaccharides were the dominant macronutrient, showing pronounced seasonal fluctuations, particularly at El Jadida. Protein content was consistently higher at El Jadida than at Sidi Rahal, with limited seasonal variation; however, lipid content remained low throughout the study. Pigments exhibited significant seasonal changes, with chlorophyll a peaking in winter, and allophycocyanin reaching maximum concentrations in summer at El Jadida and in winter at Sidi Rahal. Ethanol extractions were more efficient than aqueous methods for recovering polyphenol and flavonoid. Overall, these findings highlight the biochemical diversity of C. acicularis and its potential industrial value, supporting its development as a promising resource for biotechnological applications.

Lavandula mairei is an aromatic, and medicinal plant endemic to Morocco, mainly found in the southeastern region of the Kingdom, and used in traditional medicine for its many benefits. The purpose of the study was to identify the chemical composition of Lavandula mairei essential oil using Gas Chromatography - Tandem Mass Spectrometry, to evaluate the antimicrobial activity of the plant against bacterial strains (Staphylococcus aureus, Staphylococcus aureus MRSA, Enterococcus faecalis, Escherichia coli ATCC 25922, Escherichia coli ESBL, Klebsiella pneumoniae ATCC 700603), and two fungal strains (Aspergillus niger ATCC 10231, Candida albicans ATCC 16404) in order to measure the microbial growth inhibition zone diameter, and to determine the minimum inhibitory, bactericidal, and fungicidal concentration of the essential oil (MIC, MBC, and MFC). Concerning antioxidant activity, six assays (6DPPH, and 1ABTS Free Radical Scavenging Assay, Total Antioxidant Capacity, Hydroxyl Radical, Reducing Power, and beta-Carotene Bleaching Inhibition) were performed to determine the antioxidant potential of the essential oil. For the in silico aspect, molecular docking studies were performed to explore the potential interactions of some compounds with five microbial targets (i) Dihydropteroate synthase (1AJ0), ii) The elongation factor EF-Tu (1OB2), iii) D-alanine ligase (2I80), iv) DNA gyrase (2XCT), v) The cytochrome P450 monooxygenase (5V5Z), and 2ADMET prediction analysis was completed on a compound designated as dehydroabietinol. Carvacrol was identified as the major compound (36.38%) by analysis of the chemical composition, E. coli ATCC 25922 (MIC = 1.87 mg/mL +/- 0.00, MBC = 15.00 mg/ mL +/- 0.00), and Enterococcus faecalis (MIC = 0.23 mg/mL +/- 0.00, MBC = 5.53 mg/mL +/- 2.77) were the most sensitive to the antibacterial treatment, and Candida albicans was resistant to the essential oil. The highest antioxidant potential of the plant was observed in the beta-carotene bleaching inhibition test (IC50 = 1.55 mg/mL +/- 0.009 and 90 % of inhibition). In the in silico study, the compound dehydroabietinol provided relevant evidence for molecular docking, and this was confirmed by the ADMET prediction analysis. The results of the study were significant, and further experiments will be necessary to find out more about the biological properties of Lavandula mairei.

Diabetes is a substantial public health issue, while its prevalence continues to rise worldwide, affecting millions of persons between the ages of 20 and 80, the development of new therapeutic classes improving glycemic control and consequently micro and macrovascular complications are needed. Today, diabetes treatment is daily for life, and should not be interrupted. However, insulin secretagogues medications, and exogenous self-administration of insulin provide efficient antidiabetic effects, but their misuse leads to hypoglycemic complications besides other risks, hence the need to look for other natural products not to use solely but in concert with others types of medications. In this review, we will highlight briefly the pathophysiology of diabetes and its complications, then we will report the main bioactive macromolecules derived from various sources of natural products providing anti-diabetic properties. However, further researches need to be carried out to face the limitations hampering the development of effective natural drugs for diabetes treatment.

WO contain many elements that are toxic to the health and environment.WO discharge into the natural environment without any prior treatment can clog the filters in the water treatment stations, disrupting the biological purification processes.It is also forbidden to burn these fluids without pretreating them: their wild incineration releases dioxins and greenhouse gases.In WO recycling process, the final step is to pass it through a press filter containing BT oil treated at a ratio of 10:90.The pressed cake is then abandoned in nature, or transported to the landfill.This study focused on the reuse of BT mud cakes soiled with impurities from recycled WO.A PC study of the pulp properties, followed by a toxicological study, enabled to recommend the reuse of this slurry mud as a tight and very suitable soil covering.Thus, controlled landfills, sports fields and storage tanks for irrigation water can be coated with this compact clay, and prevent seepage into the water table.Our work is divided into two main parts: the first concerned the study of the specific characteristics of BT extracted from WO (permeability, specific surface, porosity, adhesion to the ground and durability); and the second carried out the bacteriological and eco-toxicological study of BT-WO residues, in order to prevent all negative impacts likely to reduce the quality of the soil, groundwater, human health and vegetation.

The inhibitory performance of an efficient and biodegradable Persea americana leaves extract (PALE) was inspected using electrochemical methods and surface analysis techniques in 1 M HCl. Then, antibacterial activity was assessed against Staphylococcus aureus NCTC 12493, Escherichia coli ATCC 25922, and Listeria monocytogenes ATCC 19115 using a well diffusion assay. Moreover, the minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations were determined. Additionally, the antibacterial activity was performed in silico. The experimental results showed that PALE has a more excellent inhibition standard for carbon steel in hydrochloric acid. Polarization investigations reported that as the concentration of PALE leaves extracts, the inhibitory power grew and reached a high value of 92%. PALE did act as a mixed-type inhibitor and preceded a Langmuir adsorption isotherm correctly. The scanning electron microscope (SEM) outcomes demonstrated that the PALE adsorption on the metallic surface significantly decreased its dissolution rate, resulting in a clean and seamless surface. Additionally, PALE was effective against bacterial strains, and molecular docking analysis revealed that apigenin and quercetin-3-glucoside might be the main compounds that inhibit bacterial proliferation.

Type 1 diabetes is characterized by insulin deficiency due to the destruction of pancreatic β cells, leading to hyperglycemia, which in turn induces vascular complications. In the current study, we investigated the effect of intraperitoneal administration of clove essential oil (CEO: 20 mg/kg body weight) on certain oxidative stress and glucose metabolism enzymes, as well as the expression of proinflammatory mediators. Administration of CEO to diabetic rats showed a significant decline in blood glucose levels, total cholesterol, and xanthine oxidase, compared to the streptozotocin group. Furthermore, these treated rats elicited a notable attenuation in the levels of lipid peroxides, and thiols groups in both liver and brain tissues. The activities of antioxidant and metabolic enzymes were reverted to normality in diabetic upon CEO administration. In addition to its protective effects on red blood cell hemolysis, CEO is a potent α-amylase inhibitor with an IC50 =298.0±2.75 μg/mL. Also, treatment of diabetic rats with CEO significantly reduced the iNOS expression in the spleen. Our data showed that CEO has potential beneficial effects on diabetes, which can possibly prevent the pathogenesis of diabetic micro- and macrovascular complications.

The purpose of this work was to investigate the protective effect of five essential oils (EOs); Rosmarinus officinalis, Thymus vulgaris, Origanum compactum Benth., Eucalyptus globulus Labill. and Ocimum basilicum L.; against oxidative stress induced by hydrogen peroxide in Saccharomyces cerevisiae. The chemical composition of the EOs was analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS). The in vitro antioxidant activity was evaluated and the protective effect of EOs was investigated. Yeast cells were pretreated with different concentrations of EOs (6.25-25 mu g/ml) for an hour then incubated with H2O2 (2 mM) for an additional hour. Cell viability, antioxidants (Catalase, Superoxide dismutase and Glutathione reductase) and metabolic (Succinate dehydrogenase) enzymes, as well as the level of lipid peroxidation (LPO) and protein carbonyl content (PCO) were evaluated. The chemical composition of EOs has shown the difference qualitatively and quantitatively. Indeed, O. compactum mainly contained Carvacrol, O. basilicum was mainly composed of Linalool, T. vulgaris was rich in thymol, R. officinalis had high alpha-Pinene amount and for E. globulus, eucalyptol was the major compound. The EOs of basil, oregano and thyme were found to possess the highest amount of total phenolic compounds. Moreover, they have shown the best protective effect on yeast cells against oxidative stress induced by H2O2. In addition, in a dose dependent manner of EOs in yeast medium, treated cells had lower levels of LPO, lower antioxidant and metabolic enzymes activity than cells exposed to H2O2 only. The cell viability was also improved. It seems that the studied EOs are efficient natural antioxidants, which can be exploited to protect against damages and serious diseases related to oxidative stress. (C) 2021 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.

This study aimed to evaluate the antioxidant and antidiabetic properties of clove essential oil (CEO) and to elucidate its mode of action, using selected biochemical targets, relevant to diabetes, and, specifically, its inhibitory effect on the polyol pathway. In the current study, CEO was examined for its inhibitory effects on aldose reductase in silico, in vitro, and in vivo, as well as its antioxidative activity. In silico docking studies showed that all the selected major compounds of CEO have an energy change ranging between − 5.5 and − 8.8 kcal/mol and an inhibition constant ranging between 357.08 nM and 93.12 µM. CEO significantly inhibits aldose reductase with an IC50 value of 58.55 ± 5.84 µg/mL in a noncompetitive manner. The supplementation of CEO at 20 mg/kg BW decreases retinal sorbitol dehydrogenase activity via decreased aldose reductase activity in streptozotocin (STZ)-induced diabetic Sprague Dawley rats. Moreover, diabetic rats injected with CEO have exhibited improved levels of glycemia. The IC50 values for ABTS, hydroxyl, and hydrogen peroxide scavenging activities of CEO were found to be 34.42, 277.4, and 39.99 µg/mL, respectively. Reducing power assay and phosphomolybdate assay exhibited a reduction force with the A0.5 values of 50.25 and 140.16 µg/mL, respectively. CEO potentially exerts a beneficial effect on diabetes-related complications due to its antioxidant and inhibitory effect on aldose reductase activity.

This study aimed, for the first time, to assess the purification of aldose reductase (AR) in Jaculus orientalis (Dipodidae family) kidney and to evaluate the in vitro aldose reductase inhibitory (ARI) effects of Euphorbia regisjubae (Euphorbiaceae family) aqueous and hydroethanolic extracts. Initial screening assay of the enzymatic AR activity in different jerboa states (euthermic, prehibernating and hibernating) and tissues (brain, brown adipose tissue, liver and kidneys) was assessed. Then, AR has been purified to homogeneity from the kidneys of prehibernating jerboas by a series of chromatographic technics. Furthermore, the in vitro and in silico ARI effects of E. regis-jubae (Webb & Berth) extracts, characterized by hight performance liquid chromatography (HPLC) on the purified enzyme were evaluated. Our results showed that the highest enzyme activity was detected in the kidneys, followed by white adipose tissue and the lungs of pre-hibernating jerboa. The enzyme was purified to homogeneity from jerboa kidneys during prehibernating state with a purification factor of 53.4-fold and a yield of about 6%. AR is monomeric, active in D(+)-glyceraldehyde substrate and in disodium phosphate buffer. The pH and temperature for AR were determined to be 6.5-7.5 and 35 degrees C, respectively. Results of the in vitro ARI activity was strongest with both the hydroethanolic extract (IC50 = 96.45 mu g/mL) and aqueous extract (IC50 = 140 mu g/mL). Molecular docking study indicated that catechin might be the main component in both aqueous and hydroethanolic extracts to inhibited AR. This study provides new evidence on the ARI effect of E. regis-jubae (Webb & Berth), which may be related to its phenolic constituents.

This study determined the chemical composition of Cinnamomum verum J.Presl, Cistus ladaniferus L., Laurus nobilis L., Senecio anteuphorbuim, Boswellia serrata Roxb. ex Colebr. and Schinus molle L. essential oils (EOs) by gas chromatography-mass spectrometry. Moreover, the six EOs were screened in order to reveal their antibacterial and antioxidant activities. They were initially tested against six pathogenic bacteria: Staphylococcus aureus (MRSA: methicillin-resistant Staphylococcus aureus), Escherichia coli (ESBL: extended-spectrum beta-lactamases), Enterococcus faecalis (VRE: vancomycin-resistant enterococcus), Klebsiella pneumoniae (CRK: carbapenem-resistant klebsiella), clinical isolate Staphylococcus aureus and Escherichia coli ATCC 25922. Then, the antioxidant effect was assessed by beta-carotene-linoleic acid. Cinnamomum verum J.Presl, Cistus ladaniferus L. and Laurus nobilis L. exhibited the greatest antibacterial activity except Boswellia serrata Roxb. ex Colebr. oil. But this last one showed the highest antioxidant activity (85.75%) comparable to that of BHT (98.25%). The principal component analysis between the antibacterial activity and the twenty major components of six EOs revealed that alpha-pinene, beta-pinene, 4-carene, alpha-phellandrene, 1,8-cineole, linalool, 1,3,8-p-menthatriene, alpha-terpineol, cinnamyl alcohol and cinnamaldehyde are responsible for their antibacterial activity against multi-resistant strains. From this study, the results obtained show that the EOs may constitute an alternative to the emergence of multidrug-resistant bacteria.

Response surface methodology (RSM) with a Box–Behnken design (BBD) was used to optimize the extraction of bioactive compounds from Ephedra fragilis. The results suggested that extraction with 61.93% ethanol at 44.43 °C for 15.84 h was the best solution for this combination of variables. The crude ethanol extract (CEE) obtained under optimum extraction conditions was sequentially fractionated with solvents of increasing polarity. The content of total phenolic (TP) and total flavonoid (TF) as well as the antioxidant and antiglycation activities were measured. The phytochemical fingerprint profile of the fraction with the highest activity was characterized by using RP-HPLC. The ethyl acetate fraction (EAF) had the highest TP and TF contents and exhibited the most potent antioxidant and antiglycation activities. The Pearson correlation analysis results showed that TP and TF contents were highly significantly correlated with the antioxidant and antiglycation activities. Totally, six compounds were identified in the EAF of E. fragilis, including four phenolic acids and two flavonoids. Additionally, molecular docking analysis also showed the possible connection between identified bioactive compounds and their mechanisms of action. Our results suggest new evidence on the antioxidant and antiglycation activities of E. fragilis bioactive compounds that may be applied in the treatment and prevention of aging and glycation-associated complications.

The main purpose of the present study was to investigate the ability of ethyl acetate fraction (EAF) from Ephedra fragilis to function as a protective agent against hydrogen peroxide induced oxidative damage in Tetrahymena pyriformis. The cells were preincubated with EAF (50-200 μg/mL) or ascorbic acid (50 μg/mL) for 24 h, followed by incubation with 50% H2O2 inhibitory concentration for 48 h. Cell viability was assessed using trypan exclusion method. Cell morphology and mobility, antioxidant enzymes activities (catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR)), malondialdehyde (MDA) and protein carbonyl (PCO) levels, DNA fragmentation and metabolic enzymes activities (succinate dehydrogenase (SDH) and NADPH-cytochrome c reductase (NCCR)) were investigated. Our results indicate that, pretreatment of T. pyriformis cells with EAF improved the cell viability, restored normal cell mobility and morphology, decreased the levels of both MDA and PCO level, prevent DNA fragmentation and enhanced the activity of antioxidant (CAT, SOD and GR) and metabolic (SDH and NCCR) enzymes in H2O2 damaged cells. In conclusion, these results suggest for the first time that E. fragilis is a promising source of natural antioxidants, that could offer protection against oxidative stress and should be further exploited for its use in clinical medicine.

Antibiotic resistance is a serious threat to both human and environmental ecosystems. Alternatives to conventional antimicrobial therapy such as essential oils (EOs) are needed. The aim of the present study was to investigate the chemical composition, antibacterial and antioxidant activities of some commercial EOs. In this study, six selected EOs were screened against four bacterial strains identified as resistant to antibiotics: Staphylococcus aureus, Escherichia coli, Enterococus faecalis and Klebsiella pneumonia, as well as two sensitive bacterial strains: Staphylococcus aureus and Escherichia coli. Isolated bacterial strains were characterized and identified by morphological and biochemical tests. Commercially EOs of Rosmarinus officinalis L., Zingiber officinale Roscoe., Melaleuca alternifolia Cheel., Cymbopogon winterianus, Salvia sclarea L. and Syzygium aromaticum were evaluated for their antibacterial and antioxidant activities. A GC/MS was carried out to analyze the composition of the EOs investigated. From this study, the results obtained reveal that no bacterial strain tested was resistant to any of the studied EOs. The EOs obtained from rosemary contained high amount of β-Pinene, ginger oil was rich in Zingiberene and the tea tree oil was dominated by α-Carene. Linalool was the major component in lemon and clary sage. Clove oil contained high amount of 3-Allylguaiacol. The antioxidant results showed a noticeable antioxidant activity in β-carotene-linoleic acid system in all EOs. While, in DPPH method, only clove showed activity. These antibacterial and antioxidant activities support further studies to discover new chemical structures that can inhibit the growth of multiresistant bacteria.

Artemisia campestris L. commonly known as Allal in the southeastern region of Morocco (Tigri-Tendrara) is a medicinal plant. The seeds of this species are particularly used by the local population in the preparation of traditional medicine. For the first time, essential oil obtained from hydrodistilled seeds of the Moroccan Artemisia campestris L. was analyzed by GC-FID and GC-MS. A total of fifty-four compounds amounting 86.7% of the essential oil were identified. The major components were -pinene (12.0%), spathulenol (10.8%), -pinene (7.5%), limonene (7.0%) and o-cymene (5.4%). The essential oil showed good in vitro antibacterial activity against three Gram-negative strains: E. coli, P. aeruginosa and B. vesicularis, and three Gram-positive strains S. aureus, S. epidermidis, and B. antharcis. Column chromatography fractionation of the essential oil over silica gel helped to attribute the antimicrobial activity to the oxygenated mono and sesquiterpene-rich fractions with a MIC value as low as 28.7 mg/mL.