It is known that organic selenium (Sel Plex) supplemented to pig feed mixture is significant for effective transport of essential element selenium to the food chain. It enables to create important body deposits of selenium in skeletal muscles and it also increases selenium transfer in natural metabolic form of selenomethionine into functional food. The intake of feed mixture with increased organic selenium at the dose of 0.3 mg kg-1 probably increases selenium concentration in MSM (musculus semimembranosus) - experimental selenium group 1.293 mg kg-1 and control group 0.513 mg kg-1 and in MLT (musculus longissimus thoracis) - experimental selenium group 1.364 mg kg-1 and control group 0.506 mg kg-1. The aim of this study was to evaluate the selenium and total antioxidant status (TAS) in a selected group of healthy people. Twenty-five volunteers consumed pork meat enriched with organic selenium three times a week during one month (average age in 9 men was 51.2 years, in 16 women 39.06 years, respectively). Daily selenium intake of 110 ?g was calculated by Alimenta software, version 4.3 on the basis of nutrition statement. Recommended daily selenium intake of 50 - 200 ?g was stated by the World Health Organization (WHO). During the research, the volunteers consumed pork enriched with 35 ?g of selenium. At the begining the mean selenium concentration in blood serum was at 75.41 ? 14.18?g l-1 in men, 75.21 ? 15.20?g l-1 in women. After two weeks of consumption pork enriched with selenium, the average selenium concentration in blood serum in men and women increased to 86.69 ? 11.72 ?g l-1 and 87.93 ? 16.22 ?g l-1 respectively. At the end of the study the average selenium concentration decreased in men and women to 85.75 ? 2.72 ?g l-1 to 84.07 ? 15.62 ?g l-1 respectively. In the selected group of healthy people the total antioxidant status increased from 1,68 mmol.l-1 to 1,86 mmol.l-1 after two weeks of consumption of pork enriched with selenium. However, at the end of the research the decrease in TAS was recorded. Improvement in selenium status has positive impact on human health, and thus our results could contribute to the new trends in the production of functional food.

The results of histochemical analysis of three muscles m. triceps brachii (MTB), m. longissimus thoracicus (MLT) and m. rectus femoris (MRF) of two groups of pigs created according to the genotypes MYF 4 are presented. Determination of MYF 4 genotypes was made by PCR method and for histochemical analysis was used 5 animals detected as homozygote MYF 4- AA type and 5 animals of heterozygote genotype myogenin-AB out of the total of 25 individual animals tested. The histochemical analysis proved that homozygotes AA have had bigger fat cells than heterozygotes AB in three studied muscles in average. The size of fat cells in MLT - 41.10?m or 38.50 ?m respectively dominated in both groups of animals. Percentage surface representation of interstitial tissues was higher in the studied muscles of heterozygote MYF 4-AB. The volume of ligaments was the highest in MRF (3.80% or 3.90% respectively) in both groups (myogenin - AA and AB). The average thickness was of three studied muscles muscle fibres higher at homozygote genotype myogenin-AA than in heterozygote myogenin-AB. The thickest fibres in both genotypes were in MRF (88.60 ?m, and 84.72 ?m respectively) and the lowest in MTB (73.30 and 69.40 ?m respectively). The highest values of muscle fibres thickness were detected in ?-White fibres. Their percentage surface representation corresponded to this in all three types of muscles of both studied genotype myogenin groups.