Four chromene dimers, panusimilins A-D (1-4) (racemic mixtures), and two previously undescribed chromanes (5a/5b and 6a/6b) were isolated from cultures of basidiomycete Panus similis TBRC-BCC 52578. Interestingly, synthetic compounds closely related to panusimilins A-C (1-3) were previously reported, which were produced by Lewis acid promoted dimerization of a plant-derived chromene, precocene II. In the present study, panusimilins were isolated from the culture broth extract of the fungus P. similis. The chromane monomers were shown to be a non-racemic mixture of enantiomers, and their absolute configurations were elucidated by conversion to Mosher ester derivatives. The isolated compounds were evaluated for their cytotoxic activities. Among them, 2,2-dimethyl-3,4,6-trihydroxychromane (6a/6b) showed selective activity to NCI-H187 cells (IC50 9.1 mu M). On the other hand, panusimilin B (2) exhibited antioxidant activity in the DPPH radical scavenging assay (IC50 66 mu M).

This study reports both the introduction of a new record and additional cultivation results for mushrooms of Auricularia. We have collected and established a new record of the strain MFLU24-0195 from the province of Chiang Mai, Thailand. Characterisation of this strain was based on morphological characters and DNA sequence data. Phylogenetic analyses indicate that the new strain clustered with Auricularia fibrillfera with a strong bootstrap, indicating the need for a new record to be introduced. For cultivation, two different Auricularia cornea strains and one strain of A. fibrillfera were tested on different agricultural substrates. The results showed that both A. cornea and A. fibrillfera grew well in sawdust + corn cob (A. cornea strain 1 (MFLUCC18-0346):290.87±17.62, A cornea strain 2 (MFLUCC18-0347): 280±16.54, and A. fibrillfera strain (MFLUCC24-0224): 265.97±17.45 g respectively). The first primordia were observed at 14±3.05, 15±3.21, and 15± 3.45 days, respectively. Our results therefore indicate that corn cobs can be considered for the cultivation of Auricularia.

We present Cystolepiota flavolamellata a new species discovered in the tropical regions of Thailand and Laos. Morphological characters and multigene phylogenetic analyses, which encompass ITS, LSU, rpb2, and tef1 sequences, distinguish this species. Cystolepiota flavolamellata exhibits the following unique set of characteristics: basidiomata covered with light brown to brown granules or large pyramidal squamules; free yellowish white to light yellow lamellae; broadly ellipsoid to oblong-amygdaliform to ovoid basidiospores; absence of pleurocystidia; variably shaped cheilocystidia, i.e., oblong, clavate with or without appendicular apex, conical to utriform, fusiform; an epithelium for the pileus and stipe covering; and presence of clamp connections. Molecular analyses based on multiple genes confirm its separation from other known and sequenced Cystolepiota species.

Xeromphalina belonging to the family Xeromphalinaceae and characterized as a saprobe thriving on fallen, decayed logs, has undergone taxonomic revisionary study predominantly in the Northern Hemisphere. Despite approximately 36 documented species, studies on complex groups with similar-looking species within this genus are quite fragmentary and have led to misnaming and misidentification. Recent observations have revealed one interesting species from Thailand belonging to the X. tenuipes species complex. Morphological features along phylogenetic analysis (nrITS, nrLSU, and rpb2) inferred that this species is new to science. This novel species, namely X. pseudotenuipes can be distinguished from X. tenuipes by its pileocystidia appearing brownish in 5% KOH, and hyphae of pileipellis and stipitipellis turning orangish brown in 5% KOH. Moreover, this study is the first attempt to undertake a comprehensive phylogenetic analysis encompassing all potential species of Xeromphalina for which sequences are available, aiming to identify which sequence accurately represents each species.

This article is the third in the Asian Journal of Mycology Notes series, wherein we report 50 new fungal collections distributed in two phyla, five classes, 16 orders and 35 families. The present study provides descriptions and illustrations for five new species (Acrocalymma hyaline, Allocryptovalsa aquilariae, Alternaria arida, Apoharknessia thailandica and Tatraea aseptata) and 21 new host and 23 new geographical records. All these introductions are supported by morphological data and multi-gene phylogenetic analyses. This article provides the platform to disseminate the data on fungal collections with new sequence data, which is vital for future studies. An accurate and timely report of new fungus-host or fungus-country records is necessary for the diagnostics, identification, and management of economically significant fungal groups, especially the phytopathogens.

This article is the 14th in the Fungal Diversity Notes series, wherein we report 98 taxa distributed in two phyla, seven classes, 26 orders and 50 families which are described and illustrated. Taxa in this study were collected from Australia, Brazil, Burkina Faso, Chile, China, Cyprus, Egypt, France, French Guiana, India, Indonesia, Italy, Laos, Mexico, Russia, Sri Lanka, Thailand, and Vietnam. There are 59 new taxa, 39 new hosts and new geographical distributions with one new combination. The 59 new species comprise Angustimassarina kunmingense, Asterina lopi, Asterina brigadeirensis, Bartalinia bidenticola, Bartalinia caryotae, Buellia pruinocalcarea, Coltricia insularis, Colletotrichum flexuosum, Colletotrichum thasutense, Coniochaeta caraganae, Coniothyrium yuccicola, Dematipyriforma aquatic, Dematipyriforma globispora, Dematipyriforma nilotica, Distoseptispora bambusicola, Fulvifomes jawadhuvensis, Fulvifomes malaiyanurensis, Fulvifomes thiruvannamalaiensis, Fusarium purpurea, Gerronema atrovirens, Gerronema flavum, Gerronema keralense, Gerronema kuruvense, Grammothele taiwanensis, Hongkongmyces changchunensis, Hypoxylon inaequale, Kirschsteiniothelia acutisporum, Kirschsteiniothelia crustaceum, Kirschsteiniothelia extensum, Kirschsteiniothelia septemseptatum, Kirschsteiniothelia spatiosum, Lecanora immersocalcarea, Lepiota subthailandica, Lindgomyces guizhouensis, Marthe asmius pallidoaurantiacus, Marasmius tangerinus, Neovaginatispora mangiferae, Pararamichloridium aquisubtropicum, Pestalotiopsis piraubensis, Phacidium chinaum, Phaeoisaria goiasensis, Phaeoseptum thailandicum, Pleurothecium aquisubtropicum, Pseudocercospora vernoniae, Pyrenophora verruculosa, Rhachomyces cruralis, Rhachomyces hyperommae, Rhachomyces magrinii, Rhachomyces platyprosophi, Rhizomarasmius cunninghamietorum, Skeletocutis cangshanensis, Skeletocutis subchrysella, Sporisorium anadelphiae-leptocomae, Tetraploa dashaoensis, Tomentella exiguelata, Tomentella fuscoaraneosa, Tricholomopsis lechatii, Vaginatispora flavispora and Wetmoreana blastidiocalcarea. The new combination is Torula sundara. The 39 new records on hosts and geographical distribution comprise Apiospora guiyangensis, Aplosporella artocarpi, Ascochyta medicaginicola, Astrocystis bambusicola, Athelia rolfsii, Bambusicola bambusae, Bipolaris luttrellii, Botryosphaeria dothidea, Chlorophyllum squamulosum, Colletotrichum aeschynomenes, Colletotrichum pandanicola, Coprinopsis cinerea, Corylicola italica, Curvularia alcornii, Curvularia senegalensis, Diaporthe foeniculina, Diaporthe longicolla, Diaporthe phaseolorum, Diatrypella quercina, Fusarium brachygibbosum, Helicoma aquaticum, Lepiota metulispora, Lepiota pongduadensis, Lepiota subvenenata, Melanconiella meridionalis, Monotosporella erecta, Nodulosphaeria digitalis, Palmiascoma gregariascomum, Periconia byssoides, Periconia cortaderiae, Pleopunctum ellipsoideum, Psilocybe keralensis, Scedosporium apiospermum, Scedosporium dehoogii, Scedosporium marina, Spegazzinia deightonii, Torula fici, Wiesneriomyces laurinus and Xylaria venosula. All these taxa are supported by morphological and multigene phylogenetic analyses. This article allows the researchers to publish fungal collections which areimportant for future studies. An updated, accurate and timely report of fungus-host and fungus-geography is important. We also provide an updated list of fungal taxa published in the previous fungal diversity notes. In this list, erroneous taxa and synonyms are marked and corrected accordingly.

The Global Consortium for the Classification of Fungi and fungus-like taxa is an international initiative of more than 550 mycologists to develop an electronic structure for the classification of these organisms. The members of the Consortium originate from 55 countries/regions worldwide, from a wide range of disciplines, and include senior, mid-career and early-career mycologists and plant pathologists. The Consortium will publish a biannual update of the Outline of Fungi and fungus-like taxa, to act as an international scheme for other scientists. Notes on all newly published taxa at or above the level of species will be prepared and published online on the Outline of Fungi website (https://www.outlineoffungi.org/), and these will be finally published in the biannual edition of the Outline of Fungi and fungus-like taxa. Comments on recent important taxonomic opinions on controversial topics will be included in the biannual outline. For example, 'to promote a more stable taxonomy in Fusarium given the divergences over its generic delimitation', or 'are there too many genera in the Boletales?' and even more importantly, 'what should be done with the tremendously diverse 'dark fungal taxa?' There are undeniable differences in mycologists' perceptions and opinions regarding species classification as well as the establishment of new species. Given the pluralistic nature of fungal taxonomy and its implications for species concepts and the nature of species, this consortium aims to provide a platform to better refine and stabilise fungal classification, taking into consideration views from different parties. In the future, a confidential voting system will be set up to gauge the opinions of all mycologists in the Consortium on important topics. The results of such surveys will be presented to the International Commission on the Taxonomy of Fungi (ICTF) and the Nomenclature Committee for Fungi (NCF) with opinions and percentages of votes for and against. Criticisms based on scientific evidence with regards to nomenclature, classifications, and taxonomic concepts will be welcomed, and any recommendations on specific taxonomic issues will also be encouraged; however, we will encourage professionally and ethically responsible criticisms of others' work. This biannual ongoing project will provide an outlet for advances in various topics of fungal classification, nomenclature, and taxonomic concepts and lead to a community-agreed classification scheme for the fungi and fungus-like taxa. Interested parties should contact the lead author if they would like to be involved in future outlines.

This is a case study of Lentinus in northern Thailand, including taxonomy, fruiting tests, nutritional analysis and screening for the biological activity of wild Lentinus strains.Five species (L.arcularius, L. badius, L. polychrous, L. sajor-caju and L. squarrosulus) were investigated based on morphology and a comprehensive study detailing habitat, distribution; and further including phylogenetic analyses based on ITS and LSU; and finally, each species was verified.Three active wild strains (L.arcularius, L. sajor-caju, and L. squarrosulus) were selected to observe the optimal media, temperature, and pH for mycelial growth.In addition, optimum cereal media for spawn production was studied.Four wild strains of L. squarrosulus, one strain of L. sajor-caju, and two strains of L. arcularius, were cultivated on sawdust substrate, and they were observed for fruiting body production, fresh weight, and biological efficiency.Extracts from L. sajor-caju and L. squarrosulus have been made to measure nutritional values, which comprise protein, fiber, carbohydrates and fat.Furthermore, L. sajor-caju and L. squarrosulus, were screened for biological activity for inhibition of alpha-glucosidase enzyme activity.These results on wild strains of Lentinus will be useful for further studies on the applications of Lentinus.

Fungi are a diverse eukaryotic group that is distributed throughout the world. Many mycologists have identified Thailand as a hot spot for the discovery of novel macrofungal taxa. Currently, a combination of morphological and molecular data is required for correct macrofungal identification. Traditionally, Thai macrofungi have been studied and described based on morphological characteristics. Many species of fungi have been linked to native species that had previously been identified in America and Europe. These have been included on checklists that have come to be regularly cited in many published scientific studies. However, some of these determinations are now in doubt and cannot be confirmed due to a lack of comprehensive herbarium material, fully accurate descriptions, and molecular data. Since 2005, Thai macrofungi research has greatly expanded and the identification process has significantly improved by employing molecular approaches. Many new macrofungal taxa and records have been discovered and described based on existing morphological and molecular data. This paper will briefly review the history of Thai mycology and the current status of Thai macrofungi research. Moreover, the identification of ten genera, namely Agaricus, Amanita, Astraeus, Chlorophyllum, Clitopilus, Daldinia, Ganoderma, Phlebopus, Pluteus, and Volvariella, has been updated and a new accurate list will be established based on the collection, description, and molecular data obtained from Thai native specimens.

The genus Amanita, comprising three subgenera and eleven sections, is one of the most species diverse genera within the class Agaricomycetes. To date, 54 species have been validly reported from Thailand. During surveys of macro-fungi in northern Thailand in 2019 and 2020, three Amanita species were collected and identified as the members of Amanita sect. Lepidella based on morphological and molecular data. Among the three species, two species were identified as A. aureofloccosa and A. manicata and A. aureofloccosa is reported as a new record in Thailand. Interestingly, the third species was distinct from all other known species within sect. Lepidella. We describe the third species as a new species, namely A. albicarnosa. All these three taxa are documented with morphological characteristics, a phylogenetic tree, line drawings, color photographs, and comparisons with morphologically similar taxa. A distributional map of the sampling sites and a key to the Thai species of Amanita sect. Lepidella are also provided.

Two new species of Leucoagaricus from Laos are described based on both morphological characteristics and evidence of molecular phylogeny.The first species, Leucoagaricus flavus, is well recognized by small and pale yellow to pastel yellow basidiomata; ovoid to ellipsoid basidiospores without a germ pore; clavate basidia; hyaline, clavate to narrowly clavate cheilocystidia; and a cutis pileus covering made up of cylindrical and hyaline elements.Primarily, it is distinguished from other species by its yellow basidiomata.The second species, Leucoagaricus griseosquamusus, is recognized by white basidiomata; grey to grayish-brown squamules; ellipsoidal ovoid basidiospores; clavate basidia; clavate to broadly clavate cheilocystidia; a hymeniderm pileus covering made up of a range of ellipsoidal to oblong elements with brown to the dark brown wall.Notably, each species is provided a full description, discussion on related species, field photographs, and line drawing of micro-characteristics.Furthermore, phylogenetic analyses results based on nrITS sequences are provided to show the relationship of each new species.

The Greater Mekong Sub-region (GMS) is a global biodiversity hotspot and habitat for many ecologically and economically important mushrooms species. The diversity of mushrooms in the GMS has been studied and published in different journals, and many have been documented from different countries in the GMS region but data remain mostly fragmented. A comprehensive website, https://gmsmushrooms.org/, was established to update the taxonomy, illustrations, descriptions and other useful information for mushroom species reported from the GMS into a single platform. The website is managed by expert mycologists who regularly update information as new macrofungi are introduced from the GMS.

Cystolepiota Singer is rarely studied in Southeast Asia; here, we survey and describe three new species of Cystolepiota from tropical Laos and Thailand. Cystolepiota pyramidalis is related to C. fumosifolia (Murrill) Vellinga and C. pseudofumosifolia M.L. Xu & R.L. Zhao, but it is distinguished by pale to pastel yellow lamellae. Second, Cystolepiota thailandica differs from other members in the genus by the greyish-orange granulose or powdery pileus and stipe covering made up of globose to subglobose and sphaeropedunculate elements. Furthermore, Cystolepiota rhodella is characterized by the pink-violet granulose covering of the pileus and stipe and white lamellae with distinctly violet edges. Each species is provided with a full description of the morphological characters, photos in situ, line drawings of the microcharacters, discussion of related and similar species, and molecular data.

This is the seventh in a series of Mycosphere notes in which we provide notes on the collection of fungi isolated from various hosts. In this set of notes, we introduce Pseudophialocephala as a new genus, nine new species, 27 new host or country records and five new combinations. The new species are Ceratosphaeria yunnanensis, Cytospora salicis-albae, Gymnopus bunerensis, Kalmusia cordylines, Leucoagaricus croceus, Leucoagaricus laosensis, Neopyrenochaeta shaoguanica, Pseudophialocephala cuneata and Robillarda aquatica. Pseudophialocephala humicola, Pseudophialocephala aquatica, Pseudophialocephala salinicola, Pseudophialocephala terricola and Pseudophialocephala xalapensis are introduced as new combinations. We provide new molecular data for 43 species belonging to two phyla, three classes and 15 orders. Updated phylogenetic trees for 22 families (Agaricaceae, Botryosphaeriaceae, Chaetosphaeriaceae, Coniochaetaceae, Didymosphaeriaceae, Gloeophyllaceae, Glomerellaceae, Hysteriaceae, Lophiostomataceae, Magnaporthaceae, Neopyrenochaetaceae, Omphalotacea, Phaeosphaeriaceae, Phyllostictaceae, Pleosporaceae, Saccotheciaceae, Savoryellaceae, Sporocadaceae, Stachybotryaceae, Torulaceae, Valsaceae, Physalacriaceae) and 32 genera (Alfaria, Aureobasidium, Ceratosphaeria, Collybiopsis, Colletotrichum, Comoclathris, Coniochaeta, Cytospora, Dothiorella, Gymnopus, Gymnopus, Heliocybe, Hysterium, Hysterobrevium, Kalmusia, Leptospora, Letendraea, Leucoagaricus, Mucidula, Neoleptosporella, Neopyrenochaeta, Paraleptospora, Phyllosticta, Pseudophialocephala, Rhytidhysteron, Robillarda, Savoryella, Sporocadus, Thozetella, Torula and Vaginatispora) are given.

This article is the 13th contribution in the Fungal Diversity Notes series, wherein 125 taxa from four phyla, ten classes, 31 orders, 69 families, 92 genera and three genera incertae sedis are treated, demonstrating worldwide and geographic distribution. Fungal taxa described and illustrated in the present study include three new genera, 69 new species, one new combination, one reference specimen and 51 new records on new hosts and new geographical distributions. Three new genera, Cylindrotorula ( Torulaceae ), Scolecoleotia ( Leotiales genus incertae sedis ) and Xenovaginatispora ( Lindomycetaceae ) are introduced based on distinct phylogenetic lineages and unique morphologies. Newly described species are Aspergillus lannaensis , Cercophora dulciaquae , Cladophialophora aquatica , Coprinellus punjabensis , Cortinarius alutarius , C. mammillatus , C. quercoflocculosus , Coryneum fagi , Cruentomycena uttarakhandina , Cryptocoryneum rosae , Cyathus uniperidiolus , Cylindrotorula indica , Diaporthe chamaeropicola , Didymella azollae , Diplodia alanphillipsii , Dothiora coronicola , Efibula rodriguezarmasiae , Erysiphe salicicola , Fusarium queenslandicum , Geastrum gorgonicum , G. hansagiense , Helicosporium sexualis , Helminthosporium chiangraiensis , Hongkongmyces kokensis , Hydrophilomyces hydraenae , Hygrocybe boertmannii , Hyphoderma australosetigerum , Hyphodontia yunnanensis , Khaleijomyces umikazeana , Laboulbenia divisa , Laboulbenia triarthronis , Laccaria populina , Lactarius pallidozonarius , Lepidosphaeria strobelii , Longipedicellata megafusiformis , Lophiotrema lincangensis , Marasmius benghalensis , M. jinfoshanensis , M. subtropicus , Mariannaea camelliae , Melanographium smilaxii , Microbotryum polycnemoides , Mimeomyces digitatus , Minutisphaera thailandensis , Mortierella solitaria , Mucor harpali , Nigrograna jinghongensis , Odontia huanrenensis , O. parvispina , Paraconiothyrium ajrekarii , Parafuscosporella niloticus , Phaeocytostroma yomensis , Phaeoisaria synnematicus , Phanerochaete hainanensis , Pleopunctum thailandicum , Pleurotheciella dimorphospora , Pseudochaetosphaeronema chiangraiense , Pseudodactylaria albicolonia , Rhexoacrodictys nigrospora , Russula paravioleipes , Scolecoleotia eriocamporesi , Seriascoma honghense , Synandromyces makranczyi , Thyridaria aureobrunnea , Torula lancangjiangensis , Tubeufia longihelicospora , Wicklowia fusiformispora , Xenovaginatispora phichaiensis and Xylaria apiospora . One new combination, Pseudobactrodesmium stilboideus is proposed. A reference specimen of Comoclathris permunda is designated. New host or distribution records are provided for Acrocalymma fici , Aliquandostipite khaoyaiensis , Camarosporidiella laburni , Canalisporium caribense , Chaetoscutula juniperi , Chlorophyllum demangei , C. globosum , C. hortense , Cladophialophora abundans , Dendryphion hydei , Diaporthe foeniculina , D. pseudophoenicicola , D. pyracanthae , Dictyosporium pandanicola , Dyfrolomyces distoseptatus , Ernakulamia tanakae , Eutypa flavovirens , E. lata , Favolus septatus , Fusarium atrovinosum , F. clavum , Helicosporium luteosporum , Hermatomyces nabanheensis , Hermatomyces sphaericoides , Longipedicellata aquatica , Lophiostoma caudata , L. clematidis-vitalbae , Lophiotrema hydei , L. neoarundinaria , Marasmiellus palmivorus , Megacapitula villosa , Micropsalliota globocystis , M. gracilis , Montagnula thailandica , Neohelicosporium irregulare , N. parisporum , Paradictyoarthrinium diffractum , Phaeoisaria aquatica , Poaceascoma taiwanense , Saproamanita manicata , Spegazzinia camelliae , Submersispora variabilis , Thyronectria caudata , T. mackenziei , Tubeufia chiangmaiensis , T. roseohelicospora , Vaginatispora nypae , Wicklowia submersa , Xanthagaricus necopinatus and Xylaria haemorrhoidalis . The data presented herein are based on morphological examination of fresh specimens, coupled with analysis of phylogenetic sequence data to better integrate taxa into appropriate taxonomic ranks and infer their evolutionary relationships.

A new species and a first record of Volvariella are reported from Thailand based on morphological and phylogenetic evidence. The most important features that distinguish Volvariella rostricystidiata sp. nov. are the broadly clavate to obovoid cheilocystidia with a rostrate apex and the scattered, slender and usually fusiform pleurocystidia, which lack a rostrum. Volvariella pulla, recently described from Vietnam, is reported here from northern Thailand based on morphology and sequence data. Combined ITS and nrLSU gene regions were analyzed to confirm the placement and infer the phylogenetic affinities of the two studied species.

Pleurotus is widely distributed and the most commonly cultivated edible mushroom genus worldwide. In addition to being widely consumed, some species have medical, biotechnological and environmental applications owing to their bioactivities. In this study, we report two Pleurotus species from Laos. Pleurotus djamor is reported for the first time from Laos, while P. giganteus a common species in Laos is also reported. Molecular phylogenetic analyses of the nuclear ribosomal internal transcribed spacer region (nrITS) were performed in addition to the morphological analysis to confirm their identification. Full descriptions, color photo plates, micrographs, and taxonomic comparisons are provided.

In our ongoing research on lepiotaceous taxa (Agaricaceae s.l.) in Laos and northern Thailand, we focus here on Chlorophyllum, Clarkeinda, Macrolepiota, Pseudolepiota, and Xanthagaricus. Collections were obtained from various habitats, including agricultural habitats, grasslands, and rainforests. A total of 12 taxa were examined and investigated. Of these 12, two are new for science; viz. Xanthagaricus purpureosquamulosus with brownish-grey to violet-brown squamules on a pale-violet to violet background; it shares the pileus color with X. caeruleus and X. ianthinus, but differs in other characters; and Macrolepiota excelsa, rather similar to M. procera but related to M. detersa. Two species, Pseudolepiotazangmui and Xanthagaricus necopinatus are recorded for the first time in Thailand. Four species of Chlorophyllum and a total of four species of Macrolepiota were found, viz., C. demangei and C. hortense with white basidiospores, C. molybdites and C. globosum with green basidiospores, M. detersa, M. dolichaula, the new M. excelsa, and M. velosa. Another rather common striking species is Clarkeinda trachodes, with yellow-green basidiospores. Each species is described in detail, with color photographs and line drawings. Phylogenetic analyses based on internal transcribed spacer (nrITS) region, the large subunit nuclear ribosomal (nrLSU) DNA and RNA polymerase II second largest subunit (rpb2) genes provide evidence for the placement of the species covered.

Two species of Lepiota in section Stenosporae (J. Lange) Kuhner, L. aureofulvella Sysouph., K.D. Hyde, Chukeat. & Vellinga and L. citrophylla (Berk. & Broome) Sacc., are firstly recorded from Lao PDR. Full descriptions, colour photographs and line drawings of micromorphology are provided. Furthermore, phylogenetic position of L. aureofulvella and L. citrophylla based on nrITS sequence data was confirmed and the two species were compared with related species in the section.

During our studies of the genus Lepiota in northern Thailand we collected a putatively new species with a distinct morphology and ITS nrDNA profile from Chiang Mai Province. The new species, Lepiota condylospora, is characterized by the presence of reddish brown to brownish orange or brown squamules on the pileus surface, triangular basidiospores with two lateral knobs, and a hymenidermal pileipellis composed of broadly to narrowly clavate elements. Two genetically distinct species of Lepiota section Lilaceae having triangular spores with two lateral knobs are compared with L. condylospora: Lepiota fraterna, from Papua New Guinea, differs in having larger basidiospores and cheilocystidia; while L. cristata var. macrospora, from China, has bigger basidiomata. A full description, color photographs, line drawings and a phylogenetic tree to show the position of the new species are provided.