Cucumber mosaic virus (CMV) is an important plant pathogen worldwide, which infects and causes yield losses to many solanaceous crops but rarely to potatoes. In the study reported here, we have tested the susceptibility of various potato genotypes to three different CMV strains, Pf-CMV and Fny-CMV, which belong to subgroup 1, and A9-CMV, a member of subgroup II. Eight potato genotypes were found that could be systemically infected by at least one of the three CMV strains. Furthermore, although most potato cultivars were resistant to systemic infection at 24degreesC, all became infected systemically when inoculated plants were grown at 30degreesC. These results suggested that the natural resistance that most potato crops express to CMV might be overcome under high-temperature growing conditions following infection, and that CMV resistance in potato showed virus strain specificity.

The cyc1-512 mutation in Saccharomyces cerevisiae causes a 90% reduction in the level of iso-1-cytochrome c because of the lack of a proper 3'-end-forming signal, resulting in low levels of eight aberrantly long cyc1-512 mRNAs which differ in length at their 3' termini. cyc1-512 can be suppressed by deletion of either of the nonessential genes CBC1 and CBC2, which encode the CBP80 and CBP20 subunits of the nuclear cap binding complex, respectively, or by deletion of the nonessential gene UPF1, which encodes a major component of the mRNA surveillance complex. The upf1-Delta deletion suppressed the cyc1-512 defect by diminishing degradation of the longer subset of cyc1-512 mRNAs, suggesting that downstream elements or structures occurred in the extended 3' region, similar to the downstream elements exposed by transcripts bearing premature nonsense mutations. On the other hand, suppression of cyc1-512 defects by cbc1-Delta occurred by two different mechanisms. The levels of the shorter cyc1-512 transcripts were enhanced in the cbc1-Delta mutants by promoting 3'-end formation at otherwise-weak sites, whereas the levels of the longer cyc1-512 transcripts, as well as of all mRNAs, were slightly enhanced by diminishing degradation. Furthermore, cbc1-Delta greatly suppressed the degradation of mRNAs and other phenotypes of a rat7-1 strain which is defective in mRNA export. We suggest that Cbc1p defines a novel degradation pathway that acts on mRNAs partially retained in nuclei.

Previously, a 38-base-pair (bp) region in the 3' untranslated portion of the Saccharomyces cerevisiae iso-1-cytochrome c gene, was shown to be required for both normal CYC1 mRNA 3' end formation (Zaret and Sherman, 1982), and efficient transcription termination (Russo and Sherman, 1989). In another study, specific sequences such as TATATA, TACATA, and TAGTAGTA were shown to be involved in mRNA 3' end formation in S. cerevisiae (Russo et al., 1991). In this report, an in vivo plasmid stability assay has been utilized to show that these and related sequences are also involved in transcription termination, at varying efficiencies, and in an orientation-dependent manner. For example: the sequence TATATA appeared to terminate transcription almost as efficiently as the original wild type 38-bp region; whereas, the sequences TAGATATATGTAA and TACATA were less efficient, and TTTTTTTATA had little, if any, transcription termination function. In contrast, none of these sequences appeared to terminate transcription in the reverse orientation. Therefore, it appears that certain sequence signals capable of promoting mRNA 3' end formation in yeast, are also directly involved in transcription termination.